(Circular) (Six-base) MAPSORT of: pFN21AE3380.seq Check: 6659 from: 1 to: 8101 pFN21AE3380 8101 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5911 278 Size: 53 83 186 936 4375 2468 Fragments arranged by size: 4375 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5444 1937 Size: 3507 4594 Acc65I G'GTAC_C Cuts at: 4159 4223 5422 4159 Size: 64 1199 6838 Fragments arranged by size: 6838 1199 64 AclI AA'CG_TT Cuts at: 1563 6228 6601 1563 Size: 4665 373 3063 Fragments arranged by size: 4665 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2105 2445 2826 3263 3400 3523 4124 4640 Size: 473 340 381 437 137 123 601 516 Cuts at: 4640 4689 6164 7548 7816 1632 Size: 49 1475 1384 268 1917 Fragments arranged by size: 1917 1475 1384 601 516 473 437 381 340 268 137 123 49 AflII C'TTAA_G Cuts at: 820 1017 2759 820 Size: 197 1742 6162 Fragments arranged by size: 6162 1742 197 AflIII A'CryG_T Cuts at: 1203 4464 7000 7977 1203 Size: 3261 2536 977 1327 Fragments arranged by size: 3261 2536 1327 977 AgeI A'CCGG_T Cuts at: 6932 6932 Size: 8101 AhdI GACnn_n'nnGTC Cuts at: 6830 6830 Size: 8101 AleI CACnn'nnGTG Cuts at: 1166 2240 4330 1166 Size: 1074 2090 4937 Fragments arranged by size: 4937 2090 1074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8101 AlwNI CAG_nnn'CTG Cuts at: 5844 7416 5844 Size: 1572 6529 ApaLI G'TGCA_C Cuts at: 6158 7314 6158 Size: 1156 6945 ApoI r'AATT_y Cuts at: 1493 2810 2889 3893 4189 4195 4553 5183 5410 Size: 1317 79 1004 296 6 358 630 227 Cuts at: 5410 5636 5788 7897 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1317 1004 630 358 296 227 226 152 79 6 AseI AT'TA_AT Cuts at: 160 1032 3107 4784 6655 160 Size: 872 2075 1677 1871 1606 Fragments arranged by size: 2075 1871 1677 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 AvaI C'yCGr_G Cuts at: 1870 1943 2415 5426 1870 Size: 73 472 3011 4545 Fragments arranged by size: 4545 3011 472 73 AvrII C'CTAG_G Cuts at: 4492 4492 Size: 8101 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2228 2261 2747 2780 2228 Size: 33 486 33 7549 Fragments arranged by size: 7549 486 33 33 BamHI G'GATC_C Cuts at: 4522 5431 5798 4522 Size: 909 367 6825 Fragments arranged by size: 6825 909 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4159 4223 4487 4505 5422 Size: 325 217 627 2372 64 264 18 917 Cuts at: 5422 6877 618 Size: 1455 1842 Fragments arranged by size: 2372 1842 1455 917 627 325 264 217 64 18 BanII G_rGCy'C Cuts at: 1391 5420 1391 Size: 4029 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2299 3529 4065 4073 4521 928 Size: 971 400 1230 536 8 448 4508 Fragments arranged by size: 4508 1230 971 536 448 400 8 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6292 6326 1903 Size: 34 4355 34 3678 Fragments arranged by size: 4355 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2301 5993 7203 2301 Size: 3692 1210 3199 Fragments arranged by size: 3692 3199 1210 BclI T'GATC_A Cuts at: 1548 5304 1548 Size: 3756 4345 BfrBI ATG'CAT Cuts at: 3988 4173 3988 Size: 185 7916 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6712 136 Size: 107 122 71 631 605 5040 1525 Fragments arranged by size: 5040 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3430 4414 5295 8096 3430 Size: 984 881 2801 3435 Fragments arranged by size: 3435 2801 984 881 BlpI GC'TnA_GC Cuts at: 5518 5518 Size: 8101 Bme1580I G_kGCm'C Cuts at: 1790 4492 6162 7318 1790 Size: 2702 1670 1156 2573 Fragments arranged by size: 2702 2573 1670 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8101 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4638 5832 6790 Size: 416 53 83 399 24 3221 1194 958 Cuts at: 6790 442 Size: 1753 Fragments arranged by size: 3221 1753 1194 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8101 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3609 3609 Size: 8101 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3156 4419 5055 5418 6761 1239 Size: 1917 1263 636 363 1343 2579 Fragments arranged by size: 2579 1917 1343 1263 636 363 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6226 7091 7389 6226 Size: 865 298 6938 Fragments arranged by size: 6938 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6764 882 Size: 617 5265 2219 Fragments arranged by size: 5265 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5892 493 Size: 711 4688 2702 Fragments arranged by size: 4688 2702 711 BsaBI GATnn'nnATC Cuts at: 3141 3912 4311 8095 3141 Size: 771 399 3784 3147 Fragments arranged by size: 3784 3147 771 399 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5908 6290 6925 275 Size: 53 83 186 936 4375 382 635 1451 Fragments arranged by size: 4375 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6534 6932 7206 7353 7764 6534 Size: 398 274 147 411 6871 Fragments arranged by size: 6871 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3837 3867 4207 4237 1178 Size: 30 165 30 2434 30 340 30 5042 Fragments arranged by size: 5042 2434 340 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2421 2864 4266 1186 Size: 1235 443 1402 5021 Fragments arranged by size: 5021 1402 1235 443 BseYI C'CCAG_C Cuts at: 7304 7800 7304 Size: 496 7605 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4308 1163 Size: 573 2572 4956 Fragments arranged by size: 4956 2572 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5551 6312 7340 1540 Size: 275 186 3550 761 1028 2301 Fragments arranged by size: 3550 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4273 4677 6162 6247 7318 4273 Size: 404 1485 85 1071 5056 Fragments arranged by size: 5056 1485 1071 404 85 BsiWI C'GTAC_G Cuts at: 3691 3691 Size: 8101 BsmI GAATG_Cn' Cuts at: 4175 5321 5612 5705 4175 Size: 1146 291 93 6571 Fragments arranged by size: 6571 1146 291 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3855 3855 Size: 8101 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4273 4492 4677 5420 6162 6247 7318 Size: 399 2483 219 185 743 742 85 1071 Cuts at: 7318 1391 Size: 2174 Fragments arranged by size: 2483 2174 1071 743 742 399 219 185 85 BspEI T'CCGG_A Cuts at: 7764 7764 Size: 8101 BspHI T'CATG_A Cuts at: 2518 5307 5990 7720 2518 Size: 2789 683 1730 2899 Fragments arranged by size: 2899 2789 1730 683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3242 5456 844 Size: 2398 2214 3489 Fragments arranged by size: 3489 2398 2214 BsrBI CCG'CTC Cuts at: 1394 1823 5988 1394 Size: 429 4165 3507 Fragments arranged by size: 4165 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5209 6596 6770 65 Size: 1183 3961 1387 174 1396 Fragments arranged by size: 3961 1396 1387 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4602 6745 6932 1700 Size: 110 2792 2143 187 2869 Fragments arranged by size: 2869 2792 2143 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8101 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8101 BssSI C'ACGA_G Cuts at: 6161 7173 6161 Size: 1012 7089 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8101 BstBI TT'CG_AA Cuts at: 6905 6905 Size: 8101 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8101 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2144 2011 Size: 133 7968 BstYI r'GATC_y Cuts at: 1972 2110 2283 2655 3430 4414 4522 4930 5295 Size: 138 173 372 775 984 108 408 365 Cuts at: 5295 5356 5431 5798 6183 6200 7652 7738 7750 Size: 61 75 367 385 17 1452 86 12 Cuts at: 7750 8096 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 984 775 408 385 372 367 365 346 173 138 108 86 75 61 17 12 Bsu36I CC'TnA_GG Cuts at: 3291 3291 Size: 8101 BtgI C'CryG_G Cuts at: 513 1065 2004 4154 513 Size: 552 939 2150 4460 Fragments arranged by size: 4460 2150 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7362 Fragments arranged by size: 7362 683 56 BtsI GCAGTG_nn' Cuts at: 2386 2556 2739 4252 5617 6411 6431 2386 Size: 170 183 1513 1365 794 20 4056 Fragments arranged by size: 4056 1513 1365 794 183 170 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8101 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3379 3414 529 Size: 35 2815 35 5216 Fragments arranged by size: 5216 2815 35 35 DraI TTT'AAA Cuts at: 3685 3744 3837 4194 5405 3685 Size: 59 93 357 1211 6381 Fragments arranged by size: 6381 1211 357 93 59 DraIII CAC_nnn'GTG Cuts at: 4233 4233 Size: 8101 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7108 809 Size: 507 5792 1802 Fragments arranged by size: 5792 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2598 3658 5548 Size: 54 1425 50 275 18 768 1060 1890 Cuts at: 5548 6437 8066 8 Size: 889 1629 43 Fragments arranged by size: 1890 1629 1425 1060 889 768 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5548 1537 Size: 275 3736 4090 Fragments arranged by size: 4090 3736 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2059 2439 3378 3691 4533 4665 5156 5370 Size: 706 380 939 313 842 132 491 214 Cuts at: 5370 6031 1353 Size: 661 3423 Fragments arranged by size: 3423 939 842 706 661 491 380 313 214 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6688 7062 7208 387 Size: 1064 138 5099 374 146 1280 Fragments arranged by size: 5099 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2445 2826 3156 3263 3400 3523 Size: 393 473 340 381 330 107 137 123 Cuts at: 3523 4124 4419 4640 4689 5055 5418 6164 6761 Size: 601 295 221 49 366 363 746 597 Cuts at: 6761 7548 7816 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 746 601 597 473 393 381 366 363 340 330 295 268 221 137 123 107 49 EcoNI CCTnn'n_nnAGG Cuts at: 5350 5350 Size: 8101 EcoRI G'AATT_C Cuts at: 2889 5410 5788 2889 Size: 2521 378 5202 Fragments arranged by size: 5202 2521 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2450 2555 2647 2856 3105 3430 2450 Size: 105 92 209 249 325 7121 Fragments arranged by size: 7121 325 249 209 105 92 FspI TGC'GCA Cuts at: 6607 6607 Size: 8101 HaeII r_GCGC'y Cuts at: 1678 7248 1678 Size: 5570 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2275 2307 2640 Size: 32 135 32 458 32 213 32 333 Cuts at: 2640 2672 3324 3356 3837 3869 4060 4092 5155 Size: 32 652 32 481 32 191 32 1063 Cuts at: 5155 5187 5208 5240 6742 6774 6816 6848 1373 Size: 32 21 32 1502 32 42 32 2626 Fragments arranged by size: 2626 1502 1063 652 481 458 333 213 191 135 42 32 32 32 32 32 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 2325 3719 4995 5445 5691 669 Size: 988 281 387 1394 1276 450 246 3079 Fragments arranged by size: 3079 1394 1276 988 450 387 281 246 HindIII A'AGCT_T Cuts at: 748 2223 3087 3812 4652 748 Size: 1475 864 725 840 4197 Fragments arranged by size: 4197 1475 864 840 725 HpaI GTT'AAC Cuts at: 4995 5691 4995 Size: 696 7405 KpnI G_GTAC'C Cuts at: 4163 4227 5426 4163 Size: 64 1199 6838 Fragments arranged by size: 6838 1199 64 MfeI C'AATT_G Cuts at: 5700 5700 Size: 8101 MluI A'CGCG_T Cuts at: 7977 7977 Size: 8101 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7215 7399 1305 Size: 5910 184 2007 Fragments arranged by size: 5910 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2600 3660 10 Size: 54 1425 343 768 1060 4451 Fragments arranged by size: 4451 1425 1060 768 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2240 2316 4020 4168 4330 6059 Size: 648 843 231 76 1704 148 162 1729 Cuts at: 6059 6418 6577 518 Size: 359 159 2042 Fragments arranged by size: 2042 1729 1704 843 648 359 231 162 159 148 76 MspA1I CmG'CkG Cuts at: 1606 5180 5517 6194 7342 7587 1606 Size: 3574 337 677 1148 245 2120 Fragments arranged by size: 3574 2120 1148 677 337 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4154 513 Size: 552 939 2150 4460 Fragments arranged by size: 4460 2150 939 552 NdeI CA'TA_TG Cuts at: 387 3597 4804 387 Size: 3210 1207 3684 Fragments arranged by size: 3684 3210 1207 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8101 NotI GC'GGCC_GC Cuts at: 5548 5548 Size: 8101 NsiI A_TGCA'T Cuts at: 3990 4175 3990 Size: 185 7916 NspI r_CATG'y Cuts at: 1132 1229 4468 5459 7004 1132 Size: 97 3239 991 1545 2229 Fragments arranged by size: 3239 2229 1545 991 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8101 PciI A'CATG_T Cuts at: 4464 7000 4464 Size: 2536 5565 PflMI CCAn_nnn'nTGG Cuts at: 1108 4233 5061 1108 Size: 3125 828 4148 Fragments arranged by size: 4148 3125 828 PmeI GTTT'AAAC Cuts at: 5405 5405 Size: 8101 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6170 6202 7683 7715 6170 Size: 32 1481 32 6556 Fragments arranged by size: 6556 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3714 1613 Size: 2101 6000 PsiI TTA'TAA Cuts at: 2771 2870 4355 5671 2771 Size: 99 1485 1316 5201 Fragments arranged by size: 5201 1485 1316 99 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2928 2960 2928 Size: 32 8069 PstI C_TGCA'G Cuts at: 830 2790 3136 4247 4869 5453 830 Size: 1960 346 1111 622 584 3478 Fragments arranged by size: 3478 1960 1111 622 584 346 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8101 SalI G'TCGA_C Cuts at: 1936 5443 1936 Size: 3507 4594 SapI GCTCTTCn'nnn_ Cuts at: 4665 5370 4665 Size: 705 7396 SbfI CC_TGCA'GG Cuts at: 5453 5453 Size: 8101 ScaI AGT'ACT Cuts at: 4666 6349 8061 4666 Size: 1683 1712 4706 Fragments arranged by size: 4706 1712 1683 SexAI A'CCwGG_T Cuts at: 5041 5041 Size: 8101 SfcI C'TryA_G Cuts at: 826 1045 2344 2786 3132 4243 4559 4616 4718 Size: 219 1299 442 346 1111 316 57 102 Cuts at: 4718 4756 4865 5449 6584 7265 7456 826 Size: 38 109 584 1135 681 191 1471 Fragments arranged by size: 1471 1299 1135 1111 681 584 442 346 316 219 191 109 102 57 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8101 SmaI CCC'GGG Cuts at: 1872 5428 1872 Size: 3556 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2415 2759 6205 7106 7368 820 Size: 197 926 472 344 3446 901 262 1553 Fragments arranged by size: 3446 1553 926 901 472 344 262 197 SnaBI TAC'GTA Cuts at: 493 5892 493 Size: 5399 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8101 SphI G_CATG'C Cuts at: 1132 5459 1132 Size: 4327 3774 SspI AAT'ATT Cuts at: 5 52 2971 3308 3871 4039 4796 6025 5 Size: 47 2919 337 563 168 757 1229 2081 Fragments arranged by size: 2919 2081 1229 757 563 337 168 47 StuI AGG'CCT Cuts at: 1517 3589 4390 1517 Size: 2072 801 5228 Fragments arranged by size: 5228 2072 801 StyI C'CwwG_G Cuts at: 513 1065 2004 2143 3655 3661 4154 4492 5540 Size: 552 939 139 1512 6 493 338 1048 Cuts at: 5540 513 Size: 3074 Fragments arranged by size: 3074 1512 1048 939 552 493 338 139 6 SwaI ATTT'AAAT Cuts at: 4194 4194 Size: 8101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2311 6128 6145 6298 6483 6928 325 Size: 1255 731 3817 17 153 185 445 1498 Fragments arranged by size: 3817 1498 1255 731 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2338 3274 3387 Size: 274 80 33 51 1443 361 936 113 Cuts at: 3387 4664 4740 4869 6347 8059 96 Size: 1277 76 129 1478 1712 138 Fragments arranged by size: 1712 1478 1443 1277 936 361 274 138 129 113 80 76 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4012 4044 8061 8093 4012 Size: 32 4017 32 4020 Fragments arranged by size: 4020 4017 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2447 1316 Size: 1131 6970 XbaI T'CTAG_A Cuts at: 3994 5437 3994 Size: 1443 6658 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3044 3044 Size: 8101 XhoI C'TCGA_G Cuts at: 1943 2415 1943 Size: 472 7629 XmaI C'CCGG_G Cuts at: 1870 5426 1870 Size: 3556 4545 XmnI GAAnn'nnTTC Cuts at: 3413 3459 3772 6230 3413 Size: 46 313 2458 5284 Fragments arranged by size: 5284 2458 313 46 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5909 276 Size: 53 83 186 936 4375 2468 Fragments arranged by size: 4375 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI Bpu10I BstZ17I EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI