################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AseI AsnI Asp718I AspEI AspI BamHI BbvCI BciVI BclI BfrI BglII BsaBI BseAI BsgI BsiWI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BstBI ClaI CpoI DraI Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI ScaI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AcyI 728 GGCGCC G[GA]^CG[CT]C AocI 978 CCTGAGG CC^T[ACGT]AGG ApaI 261 GGGCCC GGGCC^C AscI 1170 GGCGCGCC GG^CGCGCC Asp700I 753 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1145 TGCGCA TGC^GCA AvrII 366 CCTAGG C^CTAGG BanII 261 GGGCCC G[GA]GC[CT]^C BbeI 728 GGCGCC GGCGC^C BbrPI 910 CACGTG CAC^GTG BcgI 599 GCATCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 772 GCCGCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 366 CCTAGG C^CTAGG BsaAI 910 CACGTG [CT]AC^GT[GA] BsaHI 728 GGCGCC G[GA]^CG[CT]C BsaI 1396 GAGACC GGTCTC(1/5) BsaWI 1183 ACCGGA [AT]^CCGG[AT] BsrDI 1431 CATTGC GCAATG(2/0) BsrFI 346 GCCGGC [GA]^CCGG[CT] BssSI 652 CACGAG CACGAG(-5/-1) Bst1107I 1521 GTATAC GTA^TAC BstEII 240 GGTCACC G^GT[ACGT]ACC BstPI 240 GGTCACC G^GT[ACGT]ACC BstXI 782 CCATCGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 644 AGATCC [GA]^GATC[CT] BstZ17I 1521 GTATAC GTA^TAC Bsu36I 978 CCTGAGG CC^T[ACGT]AGG Cfr10I 346 GCCGGC [GA]^CCGG[CT] DraIII 1300 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 460 GACCCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 464 CGGCCG C^GGCCG EclXI 464 CGGCCG C^GGCCG Eco52I 464 CGGCCG C^GGCCG Eco81I 978 CCTGAGG CC^T[ACGT]AGG EcoO65I 240 GGTCACC G^GT[ACGT]ACC FauI 720 CCCGC CCCGC(4/6) FspI 1145 TGCGCA TGC^GCA Hin1I 728 GGCGCC G[GA]^CG[CT]C KasI 728 GGCGCC G^GCGCC KspI 1352 CCGCGG CCGC^GG MaeIII 241 GTCAC ^GT[ACGT]AC MflI 644 AGATCC [GA]^GATC[CT] NaeI 346 GCCGGC GCC^GGC NarI 728 GGCGCC GG^CGCC NcoI 1256 CCATGG C^CATGG NgoMIV 346 GCCGGC G^CCGGC NspI 704 ACATGC [GA]CATG^[CT] PmaCI 910 CACGTG CAC^GTG PmlI 910 CACGTG CAC^GTG SacII 1352 CCGCGG CCGC^GG SapI 1245 GCTCTTC GCTCTTC(1/4) SfiI 771 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 728 GGCGCC GGC^GCC Tsp45I 241 GTCAC ^GT[GC]AC Tsp509I 487 AATT ^AATT XhoII 644 AGATCC [GA]^GATC[CT] XmnI 753 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwI 645 GATCC GGATC(4/5) 1442 GGATC AlwNI 39 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1045 CAGCTGCTG BanI 100 GGTGCC G^G[CT][GA]CC 728 GGCGCC BcnI 462 CCCGG CC^[GC]GG 500 CCGGG BfaI 367 CTAG C^TAG 961 CTAG BlpI 405 GCTAAGC GC^T[ACGT]AGC 627 GCTGAGC Bpu1102I 405 GCTAAGC GC^T[ACGT]AGC 627 GCTGAGC BsiEI 453 CGACCG CG[GA][CT]^CG 464 CGGCCG BsmBI 813 CGTCTC CGTCTC(1/5) 21 GAGACG BsrBI 1033 CCGCTC CCGCTC(-3/-3) 613 GAGCGG BstAPI 651 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1299 GCACGATGTGC CelII 405 GCTAAGC GC^T[ACGT]AGC 627 GCTGAGC DpnII 645 GATC ^GATC 1443 GATC HgaI 360 GACGC GACGC(5/10) 1130 GCGTC MaeI 367 CTAG C^TAG 961 CTAG MaeII 305 ACGT A^CGT 911 ACGT MboI 645 GATC ^GATC 1443 GATC NciI 462 CCCGG CC^[GC]GG 500 CCGGG NdeII 645 GATC ^GATC 1443 GATC PaeR7I 943 CTCGAG C^TCGAG 1054 CTCGAG PflMI 1089 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1224 CCAGCAGGTGG Sau3AI 645 GATC ^GATC 1443 GATC SexAI 1007 ACCTGGT A^CC[AT]GGT 1016 ACCTGGT SfcI 36 CTACAG C^T[GA][CT]AG 1497 CTACAG TspRI 196 CAGTG CA[GC]TG[ACGT][ACGT]^ 1216 CAGTG Van91I 1089 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1224 CCAGCAGGTGG XhoI 943 CTCGAG C^TCGAG 1054 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccI AccII AciI AfaI AluI AspHI AvaI AvaII BalI BbsI BbvI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NlaIII NlaIV PvuII RsaI Sau96I ScrFI SfaNI SmlI StyI TaqI TseI TthHB8I