(Circular) (Six-base) MAPSORT of: pFN21AE5672.seq Check: 9642 from: 1 to: 6340 pFN21AE5672 6340 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3223 3223 Size: 6340 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2487 3291 3527 3683 1937 Size: 550 804 236 156 4594 Fragments arranged by size: 4594 804 550 236 156 Acc65I G'GTAC_C Cuts at: 3661 3661 Size: 6340 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3032 3533 4403 5787 6055 1632 Size: 1400 501 870 1384 268 1917 Fragments arranged by size: 1917 1400 1384 870 501 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 5239 6216 1203 Size: 4036 977 1327 Fragments arranged by size: 4036 1327 977 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 5069 5069 Size: 6340 AleI CACnn'nnGTG Cuts at: 1166 2810 1166 Size: 1644 4696 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2828 1184 Size: 1644 4696 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2747 2779 2747 Size: 32 6308 AlwNI CAG_nnn'CTG Cuts at: 2049 3055 4083 5655 2049 Size: 1006 1028 1572 2734 Fragments arranged by size: 2734 1572 1028 1006 ApaI G_GGCC'C Cuts at: 2270 2270 Size: 6340 ApaLI G'TGCA_C Cuts at: 4397 5553 4397 Size: 1156 5184 ApoI r'AATT_y Cuts at: 1493 3649 3875 4027 6136 1493 Size: 2156 226 152 2109 1697 Fragments arranged by size: 2156 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3176 3176 Size: 6340 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 2261 2869 2948 3059 3665 1870 Size: 73 318 608 79 111 606 4545 Fragments arranged by size: 4545 608 606 318 111 79 73 AvrII C'CTAG_G Cuts at: 2371 2371 Size: 6340 BamHI G'GATC_C Cuts at: 3670 4037 3670 Size: 367 5973 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2733 3661 5116 618 Size: 325 217 627 318 628 928 1455 1842 Fragments arranged by size: 1842 1455 928 628 627 325 318 217 BanII G_rGCy'C Cuts at: 1391 2270 3659 1391 Size: 879 1389 4072 Fragments arranged by size: 4072 1389 879 BbeI G_GCGC'C Cuts at: 2737 2737 Size: 6340 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2181 2187 2467 928 Size: 971 282 6 280 4801 Fragments arranged by size: 4801 971 282 280 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2593 2627 4531 4565 1903 Size: 34 50 34 572 34 1904 34 3678 Fragments arranged by size: 3678 1904 572 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5442 4232 Size: 1210 5130 BclI T'GATC_A Cuts at: 1548 1548 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2783 4951 136 Size: 107 122 71 631 605 1111 2168 1525 Fragments arranged by size: 2168 1525 1111 631 605 122 107 71 BglII A'GATC_T Cuts at: 6335 6335 Size: 6340 BlpI GC'TnA_GC Cuts at: 2411 2633 3757 2411 Size: 222 1124 4994 Fragments arranged by size: 4994 1124 222 Bme1580I G_kGCm'C Cuts at: 1790 2110 2270 3315 4401 5557 1790 Size: 320 160 1045 1086 1156 2573 Fragments arranged by size: 2573 1156 1086 1045 320 160 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6340 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2575 4071 5029 Size: 416 53 83 399 24 1158 1496 958 Cuts at: 5029 442 Size: 1753 Fragments arranged by size: 1753 1496 1158 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6340 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2056 2570 2785 2930 5000 1239 Size: 817 514 215 145 2070 2579 Fragments arranged by size: 2579 2070 817 514 215 145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2564 2630 3152 4465 5330 5628 2564 Size: 66 522 1313 865 298 3276 Fragments arranged by size: 3276 1313 865 522 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3395 5003 882 Size: 617 1896 1608 2219 Fragments arranged by size: 2219 1896 1608 617 BsaAI yAC'GTr Cuts at: 493 1204 2917 4131 493 Size: 711 1713 1214 2702 Fragments arranged by size: 2702 1713 1214 711 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2734 4147 4529 5164 Size: 53 83 186 936 1201 1413 382 635 Cuts at: 5164 275 Size: 1451 Fragments arranged by size: 1451 1413 1201 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3188 4773 5171 5445 5592 6003 3188 Size: 1585 398 274 147 411 3525 Fragments arranged by size: 3525 1585 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2186 2216 2747 2777 2912 Size: 30 165 30 783 30 531 30 135 Cuts at: 2912 2942 1178 Size: 30 4576 Fragments arranged by size: 4576 783 531 165 135 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2038 2204 2207 2447 2567 2630 2699 2758 Size: 852 166 3 240 120 63 69 59 Cuts at: 2758 2867 1186 Size: 109 4659 Fragments arranged by size: 4659 852 240 166 120 109 69 63 59 3 BseYI C'CCAG_C Cuts at: 2270 2557 3314 5543 6039 2270 Size: 287 757 2229 496 2571 Fragments arranged by size: 2571 2229 757 496 287 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5767 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2461 2472 3790 4551 5579 1540 Size: 275 186 460 11 1318 761 1028 2301 Fragments arranged by size: 2301 1318 1028 761 460 275 186 11 BsiHKAI G_wGCw'C Cuts at: 2523 2817 2916 3306 4401 4486 5557 2523 Size: 294 99 390 1095 85 1071 3306 Fragments arranged by size: 3306 1095 1071 390 294 99 85 BsmI GAATG_Cn' Cuts at: 3851 3944 3851 Size: 93 6247 BsmBI CGTCTCn'nnnn_ Cuts at: 2020 2824 2020 Size: 804 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2270 2523 2817 2916 3306 3315 Size: 399 320 160 253 294 99 390 9 Cuts at: 3315 3659 4401 4486 5557 1391 Size: 344 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 390 344 320 294 253 160 99 85 9 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 4229 5959 4229 Size: 1730 4610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2668 2904 2964 3223 3405 3695 844 Size: 1824 236 60 259 182 290 3489 Fragments arranged by size: 3489 1824 290 259 236 182 60 BsrBI CCG'CTC Cuts at: 1394 1823 2620 3040 4227 1394 Size: 429 797 420 1187 3507 Fragments arranged by size: 3507 1187 797 429 420 BsrDI GCAATG_nn' Cuts at: 65 1248 3435 4835 5009 65 Size: 1183 2187 1400 174 1396 Fragments arranged by size: 2187 1400 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2351 4984 5171 1700 Size: 110 541 2633 187 2869 Fragments arranged by size: 2869 2633 541 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 2665 2667 2996 3176 1925 Size: 740 2 329 180 5089 Fragments arranged by size: 5089 740 329 180 2 BssSI C'ACGA_G Cuts at: 2657 4400 5412 2657 Size: 1743 1012 3585 Fragments arranged by size: 3585 1743 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2662 3310 794 Size: 1868 648 3824 Fragments arranged by size: 3824 1868 648 BstBI TT'CG_AA Cuts at: 5144 5144 Size: 6340 BstEII G'GTnAC_C Cuts at: 1184 2245 1184 Size: 1061 5279 BstXI CCAn_nnnn'nTGG Cuts at: 2794 2794 Size: 6340 BstYI r'GATC_y Cuts at: 1972 2649 3670 4037 4422 4439 5891 5977 5989 Size: 677 1021 367 385 17 1452 86 12 Cuts at: 5989 6335 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1021 677 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3528 3528 Size: 6340 Bsu36I CC'TnA_GG Cuts at: 2984 2984 Size: 6340 BtgI C'CryG_G Cuts at: 513 1065 2134 2823 2847 3261 3357 3375 513 Size: 552 1069 689 24 414 96 18 3478 Fragments arranged by size: 3478 1069 689 552 414 96 24 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3241 487 Size: 683 56 2015 3586 Fragments arranged by size: 3586 2015 683 56 BtsI GCAGTG_nn' Cuts at: 3856 4650 4670 3856 Size: 794 20 5526 Fragments arranged by size: 5526 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6305 DraI TTT'AAA Cuts at: 3644 3644 Size: 6340 DraIII CAC_nnn'GTG Cuts at: 3310 3310 Size: 6340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2471 5347 809 Size: 507 1155 2876 1802 Fragments arranged by size: 2876 1802 1155 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2317 2349 2382 Size: 54 1425 50 275 18 487 32 33 Cuts at: 2382 2469 2775 3027 3091 3787 4676 6305 8 Size: 87 306 252 64 696 889 1629 43 Fragments arranged by size: 1629 1425 889 696 487 306 275 252 87 64 54 50 43 33 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2469 3787 1537 Size: 275 657 1318 4090 Fragments arranged by size: 4090 1318 657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2217 2705 3257 4270 1353 Size: 864 488 552 1013 3423 Fragments arranged by size: 3423 1013 864 552 488 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2705 4927 5301 5447 387 Size: 1064 138 1116 2222 374 146 1280 Fragments arranged by size: 2222 1280 1116 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2056 2570 2785 2930 3032 3533 4403 Size: 393 424 514 215 145 102 501 870 Cuts at: 4403 5000 5787 6055 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 870 787 597 514 501 424 393 268 215 145 102 EcoO109I rG'GnC_Cy Cuts at: 2046 2266 2267 2046 Size: 220 1 6119 Fragments arranged by size: 6119 220 1 EcoRI G'AATT_C Cuts at: 3649 4027 3649 Size: 378 5962 FspI TGC'GCA Cuts at: 3152 4846 3152 Size: 1694 4646 FspAI rTGC'GCAy Cuts at: 3152 3152 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 2737 2834 2840 2992 3006 5487 1678 Size: 1059 97 6 152 14 2481 2531 Fragments arranged by size: 2531 2481 1059 152 97 14 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4981 5013 5055 5087 1373 Size: 32 135 32 3409 32 42 32 2626 Fragments arranged by size: 3409 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3684 3930 669 Size: 988 281 1746 246 3079 Fragments arranged by size: 3079 1746 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6340 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KasI G'GCGC_C Cuts at: 2733 2733 Size: 6340 KpnI G_GTAC'C Cuts at: 3665 3665 Size: 6340 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5454 5638 1305 Size: 4149 184 2007 Fragments arranged by size: 4149 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2319 2384 3029 10 Size: 54 1425 343 487 65 645 3321 Fragments arranged by size: 3321 1425 645 487 343 65 54 MslI CAynn'nnrTG Cuts at: 518 1166 2792 2810 3260 4298 4657 4816 518 Size: 648 1626 18 450 1038 359 159 2042 Fragments arranged by size: 2042 1626 1038 648 450 359 159 18 MspA1I CmG'CkG Cuts at: 1606 2332 2482 2731 3052 3359 3756 4433 5581 Size: 726 150 249 321 307 397 677 1148 Cuts at: 5581 5826 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 726 677 397 321 307 249 245 150 NaeI GCC'GGC Cuts at: 2353 2353 Size: 6340 NarI GG'CG_CC Cuts at: 2734 2734 Size: 6340 NcoI C'CATG_G Cuts at: 513 1065 3261 513 Size: 552 2196 3592 Fragments arranged by size: 3592 2196 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6340 NgoMIV G'CCGG_C Cuts at: 2351 2351 Size: 6340 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6340 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 2713 3698 5243 1132 Size: 97 1484 985 1545 2229 Fragments arranged by size: 2229 1545 1484 985 97 PasI CC'CwG_GG Cuts at: 1348 2101 1348 Size: 753 5587 PciI A'CATG_T Cuts at: 5239 5239 Size: 6340 PflMI CCAn_nnn'nTGG Cuts at: 1108 3100 3235 1108 Size: 1992 135 4213 Fragments arranged by size: 4213 1992 135 PfoI T'CCnGG_A Cuts at: 2033 2547 2907 2033 Size: 514 360 5466 Fragments arranged by size: 5466 514 360 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PmlI CAC'GTG Cuts at: 2917 2917 Size: 6340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2747 2779 4409 4441 5922 5954 2747 Size: 32 1630 32 1481 32 3133 Fragments arranged by size: 3133 1630 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6340 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PspOMI G'GGCC_C Cuts at: 2266 2266 Size: 6340 PspXI vC'TCGA_Gb Cuts at: 2948 3059 2948 Size: 111 6229 PstI C_TGCA'G Cuts at: 830 3692 830 Size: 2862 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 PvuII CAG'CTG Cuts at: 2332 2482 3052 2332 Size: 150 570 5620 Fragments arranged by size: 5620 570 150 SacII CC_GC'GG Cuts at: 3360 3360 Size: 6340 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SapI GCTCTTCn'nnn_ Cuts at: 3257 3257 Size: 6340 SbfI CC_TGCA'GG Cuts at: 3692 3692 Size: 6340 ScaI AGT'ACT Cuts at: 4588 6300 4588 Size: 1712 4628 SexAI A'CCwGG_T Cuts at: 3012 3021 3012 Size: 9 6331 SfcI C'TryA_G Cuts at: 826 1045 2041 3502 3688 4823 5504 5695 826 Size: 219 996 1461 186 1135 681 191 1471 Fragments arranged by size: 1471 1461 1135 996 681 219 191 186 SfiI GGCCn_nnn'nGGCC Cuts at: 2783 2783 Size: 6340 SfoI GGC'GCC Cuts at: 2735 2735 Size: 6340 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 3667 1872 Size: 1795 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2579 2645 2948 3059 3167 4444 Size: 197 926 636 66 303 111 108 1277 Cuts at: 4444 5345 5607 820 Size: 901 262 1553 Fragments arranged by size: 1553 1277 926 901 636 303 262 197 111 108 66 SnaBI TAC'GTA Cuts at: 493 4131 493 Size: 3638 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 3698 1132 Size: 2566 3774 SspI AAT'ATT Cuts at: 5 52 4264 5 Size: 47 4212 2081 Fragments arranged by size: 4212 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6340 StyI C'CwwG_G Cuts at: 513 1065 2371 2781 3261 3779 513 Size: 552 1306 410 480 518 3074 Fragments arranged by size: 3074 1306 552 518 480 410 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2093 2303 4367 4384 4537 4722 5167 Size: 1255 513 210 2064 17 153 185 445 Cuts at: 5167 325 Size: 1498 Fragments arranged by size: 2064 1498 1255 513 445 210 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2583 4586 6298 Size: 274 80 33 51 1443 606 2003 1712 Cuts at: 6298 96 Size: 138 Fragments arranged by size: 2003 1712 1443 606 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2186 2218 2912 2944 6300 6332 2186 Size: 32 694 32 3356 32 2194 Fragments arranged by size: 3356 2194 694 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6340 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XhoI C'TCGA_G Cuts at: 1943 2948 3059 1943 Size: 1005 111 5224 Fragments arranged by size: 5224 1005 111 XmaI C'CCGG_G Cuts at: 1870 3665 1870 Size: 1795 4545 XmnI GAAnn'nnTTC Cuts at: 2762 4469 2762 Size: 1707 4633 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI BaeI BbvCI BfrBI BplI Bpu10I BsiWI EcoICRI EcoNI EcoRV FalI FseI NruI NsiI PacI PpuMI PsrI RsrII SacI SanDI SrfI SwaI XcmI