################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII AccI AccIII AcsI AflII AgeI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI AvrII BalI BamHI BbrPI BcgI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaI BsaWI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI Ksp632I MfeI MflI MluI MluNI MroI MscI MslI MunI NdeI NheI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp509I Van91I XhoI XhoII ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 1100 GGTACC G^GTACC AclI 1042 AACGTT AA^CGTT AflIII 1013 ACACGT A^C[GA][CT]GT AlwNI 899 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 847 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1100 GGTACC G^GTACC AspI 304 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 207 TGCGCA TGC^GCA BbeI 60 GGCGCC GGCGC^C BciVI 610 GGATAC GTATCC(6/5) BglI 373 GCCGAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1302 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 156 CTGCAC GTGCAG(16/14) BsiEI 386 CGGCCG CG[GA][CT]^CG BsmAI 476 GAGAC GTCTC(1/5) BsmBI 476 GAGACG CGTCTC(1/5) Bsp1407I 26 TGTACA T^GTACA BsrBI 187 GAGCGG CCGCTC(-3/-3) BsrFI 224 GCCGGC [GA]^CCGG[CT] BsrGI 26 TGTACA T^GTACA BstXI 1052 CCAGTCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 224 GCCGGC [GA]^CCGG[CT] DraIII 1167 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 386 CGGCCG C^GGCCG EclXI 386 CGGCCG C^GGCCG Eco52I 386 CGGCCG C^GGCCG EcoNI 690 CCTGATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 207 TGCGCA TGC^GCA KasI 60 GGCGCC G^GCGCC KpnI 1100 GGTACC GGTAC^C KspI 383 CCGCGG CCGC^GG MamI 1302 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 763 TTAA T^TAA NaeI 224 GCCGGC GCC^GGC NarI 60 GGCGCC GG^CGCC NcoI 102 CCATGG C^CATGG NgoMIV 224 GCCGGC G^CCGGC NotI 385 GCGGCCGC GC^GGCCGC PflFI 304 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 1042 AACGTT AA^CGTT PvuII 1204 CAGCTG CAG^CTG SacII 383 CCGCGG CCGC^GG SexAI 830 ACCTGGT A^CC[AT]GGT SfoI 60 GGCGCC GGC^GCC SmaI 421 CCCGGG CCC^GGG SspBI 26 TGTACA T^GTACA TfiI 681 GAATC G^A[AT]TC Tru9I 763 TTAA T^TAA Tth111I 304 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1393 TCTAGA T^CTAGA XmaCI 421 CCCGGG C^CCGGG XmaI 421 CCCGGG C^CCGGG XmnI 847 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 27 GTAC GT^AC 1101 GTAC AhdI 478 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1369 GACGAGAGGTC AlwI 866 GATCC GGATC(4/5) 991 GGATC ApaI 286 GGGCCC GGGCC^C 699 GGGCCC AspEI 478 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1369 GACGAGAGGTC AspHI 802 GAGCTC G[AT]GC[AT]^C 954 GAGCTC AvaII 828 GGACC G^G[AT]CC 1211 GGTCC BanI 60 GGCGCC G^G[CT][GA]CC 1100 GGTACC BbvCI 1113 GCTGAGG CCTCAGC(-5/-2) 1206 GCTGAGG BseRI 1383 CTCCTC GAGGAG(10/8) 531 GAGGAG BsiHKAI 802 GAGCTC G[AT]GC[AT]^C 954 GAGCTC BspMI 160 ACCTGC ACCTGC(4/8) 210 GCAGGT DpnII 866 GATC ^GATC 992 GATC EaeI 380 TGGCCG [CT]^GGCC[GA] 386 CGGCCG Eam1105I 478 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1369 GACGAGAGGTC EcoT14I 102 CCATGG C^C[AT][AT]GG 1058 CCTTGG MaeII 1015 ACGT A^CGT 1043 ACGT MboI 866 GATC ^GATC 992 GATC NdeII 866 GATC ^GATC 992 GATC PpuMI 827 GGGACCT [GA]G^G[AT]CC[CT] 1210 AGGTCCC RsaI 27 GTAC GT^AC 1101 GTAC SacI 802 GAGCTC GAGCT^C 954 GAGCTC Sau3AI 866 GATC ^GATC 992 GATC SfaNI 1110 GATGC GCATC(5/9) 173 GCATC StyI 102 CCATGG C^C[AT][AT]GG 1058 CCTTGG TspRI 510 CAGTG CA[GC]TG[ACGT][ACGT]^ 522 CACTG XcmI 236 CCAACGAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 977 CCAAATGGTCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AcyI AluI AvaI BanII BbsI BbvI BcnI BfaI BmyI BpuAI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI Sau96I ScrFI SfcI SmlI TaqI TseI Tsp45I TthHB8I