(Circular) (Six-base) MAPSORT of: pFN21AB7044.seq Check: 804 from: 1 to: 6121 pFN21AB7044 6121 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2174 2206 2174 Size: 32 6089 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3464 1937 Size: 1527 4594 Acc65I G'GTAC_C Cuts at: 3105 3442 3105 Size: 337 5784 AclI AA'CG_TT Cuts at: 1563 3048 4248 4621 1563 Size: 1485 1200 373 3063 Fragments arranged by size: 3063 1485 1200 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2487 2497 2716 3244 4184 5568 5836 1632 Size: 855 10 219 528 940 1384 268 1917 Fragments arranged by size: 1917 1384 940 855 528 268 219 10 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 3018 5020 5997 1203 Size: 1815 2002 977 1327 Fragments arranged by size: 2002 1815 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 2488 3379 4850 2488 Size: 891 1471 3759 Fragments arranged by size: 3759 1471 891 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6121 AlwNI CAG_nnn'CTG Cuts at: 2909 3864 5436 2909 Size: 955 1572 3594 Fragments arranged by size: 3594 1572 955 ApaI G_GGCC'C Cuts at: 2295 2708 2295 Size: 413 5708 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 3430 3656 3808 5917 1493 Size: 1937 226 152 2109 1697 Fragments arranged by size: 2109 1937 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 2426 2645 2710 3446 1870 Size: 73 483 219 65 736 4545 Fragments arranged by size: 4545 736 483 219 73 65 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3089 3122 3089 Size: 33 6088 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 3105 3442 4897 618 Size: 325 217 627 278 1040 337 1455 1842 Fragments arranged by size: 1842 1455 1040 627 337 325 278 217 BanII G_rGCy'C Cuts at: 1391 2295 2434 2588 2708 2811 2963 3152 3440 Size: 904 139 154 120 103 152 189 288 Cuts at: 3440 1391 Size: 4072 Fragments arranged by size: 4072 904 288 189 154 152 139 120 103 BbeI G_GCGC'C Cuts at: 2069 2069 Size: 6121 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2503 2970 3250 3303 928 Size: 971 604 467 280 53 3746 Fragments arranged by size: 3746 971 604 467 280 53 BbvCI CC'TCA_GC Cuts at: 3119 3212 3119 Size: 93 6028 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 1903 Size: 34 2375 34 3678 Fragments arranged by size: 3678 2375 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2609 4013 5223 2609 Size: 1404 1210 3507 Fragments arranged by size: 3507 1404 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2384 4732 136 Size: 107 122 71 631 605 712 2348 1525 Fragments arranged by size: 2348 1525 712 631 605 122 107 71 BglII A'GATC_T Cuts at: 6116 6116 Size: 6121 BlpI GC'TnA_GC Cuts at: 3538 3538 Size: 6121 Bme1580I G_kGCm'C Cuts at: 1790 2295 2708 2940 4182 5338 1790 Size: 505 413 232 1242 1156 2573 Fragments arranged by size: 2573 1242 1156 505 413 232 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3051 3214 3234 Size: 416 53 83 399 24 1634 163 20 Cuts at: 3234 3852 4810 442 Size: 618 958 1753 Fragments arranged by size: 1753 1634 958 618 416 399 163 83 53 24 20 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2725 2725 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4781 1239 Size: 3542 2579 Bpu10I CC'TnA_GC Cuts at: 3119 3212 3119 Size: 93 6028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2111 2801 2825 2977 4246 5111 5409 2111 Size: 690 24 152 1269 865 298 2823 Fragments arranged by size: 2823 1269 865 690 298 152 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4784 882 Size: 617 3285 2219 Fragments arranged by size: 3285 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 3311 6115 3311 Size: 2804 3317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2112 2310 3928 Size: 53 83 186 936 533 46 198 1618 Cuts at: 3928 4310 4945 275 Size: 382 635 1451 Fragments arranged by size: 1618 1451 936 635 533 382 198 186 83 53 46 BsaWI w'CCGG_w Cuts at: 4554 4952 5226 5373 5784 4554 Size: 398 274 147 411 4891 Fragments arranged by size: 4891 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5896 Fragments arranged by size: 5896 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2551 3379 1186 Size: 1365 828 3928 Fragments arranged by size: 3928 1365 828 BseYI C'CCAG_C Cuts at: 3068 3093 5324 5820 3068 Size: 25 2231 496 3369 Fragments arranged by size: 3369 2231 496 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2146 1163 Size: 573 410 5138 Fragments arranged by size: 5138 573 410 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2394 3571 4332 5360 1540 Size: 275 186 393 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 393 275 186 BsiHKAI G_wGCw'C Cuts at: 2811 2963 4182 4267 5338 2811 Size: 152 1219 85 1071 3594 Fragments arranged by size: 3594 1219 1071 152 85 BsmI GAATG_Cn' Cuts at: 3632 3725 3632 Size: 93 6028 BsmBI CGTCTCn'nnnn_ Cuts at: 2475 2475 Size: 6121 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2295 2434 2588 2708 2811 2940 2963 Size: 399 505 139 154 120 103 129 23 Cuts at: 2963 3152 3440 4182 4267 5338 1391 Size: 189 288 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 505 399 288 189 154 139 129 120 103 85 23 BspEI T'CCGG_A Cuts at: 5784 5784 Size: 6121 BspHI T'CATG_A Cuts at: 4010 5740 4010 Size: 1730 4391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2174 2206 3476 844 Size: 1330 32 1270 3489 Fragments arranged by size: 3489 1330 1270 32 BsrBI CCG'CTC Cuts at: 1394 1823 2194 4008 1394 Size: 429 371 1814 3507 Fragments arranged by size: 3507 1814 429 371 BsrDI GCAATG_nn' Cuts at: 65 1248 4616 4790 65 Size: 1183 3368 174 1396 Fragments arranged by size: 3368 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2229 4765 4952 1700 Size: 110 419 2536 187 2869 Fragments arranged by size: 2869 2536 419 187 110 BsrGI T'GTAC_A Cuts at: 96 2031 96 Size: 1935 4186 BssHII G'CGCG_C Cuts at: 1925 2091 2093 2278 1925 Size: 166 2 185 5768 Fragments arranged by size: 5768 185 166 2 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6121 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6121 BstXI CCAn_nnnn'nTGG Cuts at: 3064 3064 Size: 6121 BstYI r'GATC_y Cuts at: 1972 3451 3818 4203 4220 5672 5758 5770 6116 Size: 1479 367 385 17 1452 86 12 346 Cuts at: 6116 1972 Size: 1977 Fragments arranged by size: 1977 1479 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2060 2107 2388 2601 2847 3291 513 Size: 552 995 47 281 213 246 444 3343 Fragments arranged by size: 3343 995 552 444 281 246 213 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 3637 4431 4451 3637 Size: 794 20 5307 Fragments arranged by size: 5307 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2806 2841 529 Size: 35 2242 35 3809 Fragments arranged by size: 3809 2242 35 35 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DraIII CAC_nnn'GTG Cuts at: 3177 3177 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2385 2391 3568 Size: 54 1425 50 275 18 555 6 1177 Cuts at: 3568 4457 6086 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1177 889 555 275 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2391 3568 1537 Size: 275 579 1177 4090 Fragments arranged by size: 4090 1177 579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4051 1353 Size: 2698 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2809 2961 2809 Size: 152 5969 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2497 2716 3244 4184 4781 5568 Size: 393 855 10 219 528 940 597 787 Cuts at: 5568 5836 1239 Size: 268 1524 Fragments arranged by size: 1524 940 855 787 597 528 393 268 219 10 EcoNI CCTnn'n_nnAGG Cuts at: 2699 2699 Size: 6121 EcoO109I rG'GnC_Cy Cuts at: 2291 2292 2704 2705 2833 3216 2291 Size: 1 412 1 128 383 5196 Fragments arranged by size: 5196 412 383 128 1 1 EcoRI G'AATT_C Cuts at: 3430 3808 3430 Size: 378 5743 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2492 2587 2492 Size: 95 6026 FspI TGC'GCA Cuts at: 2214 4627 2214 Size: 2413 3708 HaeII r_GCGC'y Cuts at: 1678 2069 2225 2847 5268 1678 Size: 391 156 622 2421 2531 Fragments arranged by size: 2531 2421 622 391 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 2582 2607 2614 Size: 32 135 32 902 32 76 25 7 Cuts at: 2614 2639 2707 2739 2862 2894 3365 3397 4762 Size: 25 68 32 123 32 471 32 1365 Cuts at: 4762 4794 4836 4868 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1365 902 471 135 123 76 68 42 32 32 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 3465 3711 669 Size: 988 281 1527 246 3079 Fragments arranged by size: 3079 1527 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KasI G'GCGC_C Cuts at: 2065 2065 Size: 6121 KpnI G_GTAC'C Cuts at: 3109 3446 3109 Size: 337 5784 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2387 5235 5419 1305 Size: 1082 2848 184 2007 Fragments arranged by size: 2848 2007 1082 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4299 Fragments arranged by size: 4299 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4079 4438 4597 518 Size: 648 2913 359 159 2042 Fragments arranged by size: 2913 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2200 2390 3112 3211 3537 4214 5362 5607 Size: 594 190 722 99 326 677 1148 245 Cuts at: 5607 1606 Size: 2120 Fragments arranged by size: 2120 1148 722 677 594 326 245 190 99 NaeI GCC'GGC Cuts at: 2231 2231 Size: 6121 NarI GG'CG_CC Cuts at: 2066 2066 Size: 6121 NcoI C'CATG_G Cuts at: 513 1065 2107 513 Size: 552 1042 4527 Fragments arranged by size: 4527 1042 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NgoMIV G'CCGG_C Cuts at: 2229 2229 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 2391 3568 2391 Size: 1177 4944 NspI r_CATG'y Cuts at: 1132 1229 3479 5024 1132 Size: 97 2250 1545 2229 Fragments arranged by size: 2250 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6121 PciI A'CATG_T Cuts at: 5020 5020 Size: 6121 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6121 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2833 3216 2833 Size: 383 5738 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 3691 3691 Size: 6121 PspOMI G'GGCC_C Cuts at: 2291 2704 2291 Size: 413 5708 PstI C_TGCA'G Cuts at: 830 2076 2439 3081 3198 3473 830 Size: 1246 363 642 117 275 3478 Fragments arranged by size: 3478 1246 642 363 275 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 3211 3211 Size: 6121 SacI G_AGCT'C Cuts at: 2811 2963 2811 Size: 152 5969 SacII CC_GC'GG Cuts at: 2391 2391 Size: 6121 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SexAI A'CCwGG_T Cuts at: 2835 2835 Size: 6121 SfcI C'TryA_G Cuts at: 826 1045 2072 2435 3077 3194 3469 4604 5285 Size: 219 1027 363 642 117 275 1135 681 Cuts at: 5285 5476 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1027 681 642 363 275 219 191 117 SfoI GGC'GCC Cuts at: 2067 2067 Size: 6121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 2428 3448 1872 Size: 556 1020 4545 Fragments arranged by size: 4545 1020 556 SmlI C'TyrA_G Cuts at: 820 1017 1943 2126 2804 2816 2956 4225 5126 Size: 197 926 183 678 12 140 1269 901 Cuts at: 5126 5388 820 Size: 262 1553 Fragments arranged by size: 1553 1269 926 901 678 262 197 183 140 12 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 3479 1132 Size: 2347 3774 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6121 StyI C'CwwG_G Cuts at: 513 1065 2107 3063 3560 513 Size: 552 1042 956 497 3074 Fragments arranged by size: 3074 1042 956 552 497 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3315 4148 4165 4318 4503 4948 325 Size: 1255 1735 833 17 153 185 445 1498 Fragments arranged by size: 1735 1498 1255 833 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 4367 6079 Size: 274 80 33 51 1443 54 2336 1712 Cuts at: 6079 96 Size: 138 Fragments arranged by size: 2336 1712 1443 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6081 6113 6081 Size: 32 6089 Tth111I GACn'n_nGTC Cuts at: 1316 2312 1316 Size: 996 5125 XbaI T'CTAG_A Cuts at: 3398 3457 3398 Size: 59 6062 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2989 2248 Size: 741 5380 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 2426 3446 1870 Size: 556 1020 4545 Fragments arranged by size: 4545 1020 556 XmnI GAAnn'nnTTC Cuts at: 2856 4250 2856 Size: 1394 4727 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI