################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AocI Aor51HI AscI Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI Tth111I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Alw44I 31 GTGCAC G^TGCAC AlwI 1271 GGATC GGATC(4/5) ApaLI 31 GTGCAC G^TGCAC AseI 649 ATTAAT AT^TAAT AsnI 649 ATTAAT AT^TAAT BalI 69 TGGCCA TGG^CCA BanI 225 GGCACC G^G[CT][GA]CC BcgI 33 GCACTTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsiWI 746 CGTACG C^GTACG BsmBI 368 GAGACG CGTCTC(1/5) Bsp1407I 1336 TGTACA T^GTACA BspMI 200 ACCTGC ACCTGC(4/8) BsrDI 1991 GCAATG GCAATG(2/0) BsrGI 1336 TGTACA T^GTACA BstYI 1271 GGATCT [GA]^GATC[CT] DraIII 840 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 69 TGGCCA [CT]^GGCC[GA] EarI 181 GAAGAG CTCTTC(1/4) EcoRV 628 GATATC GAT^ATC EcoT22I 1147 ATGCAT ATGCA^T HgaI 496 GACGC GACGC(5/10) Ksp632I 181 GAAGAG CTCTTC(1/4) MaeII 371 ACGT A^CGT MflI 1271 GGATCT [GA]^GATC[CT] MluNI 69 TGGCCA TGG^CCA MscI 69 TGGCCA TGG^CCA NsiI 1147 ATGCAT ATGCA^T PleI 1284 GAGTC GAGTC(4/5) PshAI 318 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 649 ATTAAT AT^TAAT PstI 202 CTGCAG CTGCA^G SapI 181 GAAGAGC GCTCTTC(1/4) SbfI 201 CCTGCAGG CCTGCA^GG SmaI 415 CCCGGG CCC^GGG Sse8387I 201 CCTGCAGG CCTGCA^GG SspBI 1336 TGTACA T^GTACA SwaI 1404 ATTTAAAT ATTT^AAAT TaqI 197 TCGA T^CGA Tsp45I 427 GTGAC ^GT[GC]AC TthHB8I 197 TCGA T^CGA XhoII 1271 GGATCT [GA]^GATC[CT] XmaCI 415 CCCGGG C^CCGGG XmaI 415 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 747 GTAC GT^AC 1337 GTAC AlwNI 55 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG 777 CAGGCTCTG ApaI 77 GGGCCC GGGCC^C 1814 GGGCCC AvaI 41 CTCGGG C^[CT]CG[GA]G 415 CCCGGG BbsI 1909 GAAGAC GAAGAC(2/6) 772 GTCTTC BpuAI 1909 GAAGAC GAAGAC(2/6) 772 GTCTTC BsgI 755 GTGCAG GTGCAG(16/14) 1746 GTGCAG BsmAI 368 GAGAC GTCTC(1/5) 466 GAGAC BsoBI 41 CTCGGG C^[CT]CG[GA]G 415 CCCGGG DpnII 159 GATC ^GATC 1272 GATC DraI 832 TTTAAA TTT^AAA 1405 TTTAAA DsaI 187 CCATGG C^C[GA][CT]GG 732 CCATGG EcoRI 1214 GAATTC G^AATTC 1961 GAATTC HphI 1731 GGTGA GGTGA(8/7) 488 TCACC MaeIII 10 GTAAC ^GT[ACGT]AC 427 GTGAC MboI 159 GATC ^GATC 1272 GATC NcoI 187 CCATGG C^CATGG 732 CCATGG NdeII 159 GATC ^GATC 1272 GATC PpuMI 191 GGGTCCT [GA]G^G[AT]CC[CT] 232 AGGACCT PvuII 1042 CAGCTG CAG^CTG 1294 CAGCTG RsaI 747 GTAC GT^AC 1337 GTAC Sau3AI 159 GATC ^GATC 1272 GATC SmlI 954 CTCAAG C^T[CT][GA]AG 2022 CTCAAG StuI 275 AGGCCT AGG^CCT 1106 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcsI AluI ApoI Asp700I AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII ItaI MaeI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV SacI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI XmnI