(Circular) (Six-base) MAPSORT of: pFN21AE1178.seq Check: 588 from: 1 to: 6763 pFN21AE1178 6763 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4573 278 Size: 53 83 186 936 3037 2468 Fragments arranged by size: 3037 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4106 1937 Size: 2169 4594 Acc65I G'GTAC_C Cuts at: 4084 4084 Size: 6763 AclI AA'CG_TT Cuts at: 1563 4890 5263 1563 Size: 3327 373 3063 Fragments arranged by size: 3327 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2613 2764 3016 3100 3505 3589 3673 3757 Size: 981 151 252 84 405 84 84 84 Cuts at: 3757 4826 6210 6478 1632 Size: 1069 1384 268 1917 Fragments arranged by size: 1917 1384 1069 981 405 268 252 151 84 84 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6566 AflIII A'CryG_T Cuts at: 1203 5662 6639 1203 Size: 4459 977 1327 Fragments arranged by size: 4459 1327 977 AgeI A'CCGG_T Cuts at: 5594 5594 Size: 6763 AhdI GACnn_n'nnGTC Cuts at: 5492 5492 Size: 6763 AleI CACnn'nnGTG Cuts at: 1166 2118 3866 1166 Size: 952 1748 4063 Fragments arranged by size: 4063 1748 952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6763 AlwNI CAG_nnn'CTG Cuts at: 2065 2787 4506 6078 2065 Size: 722 1719 1572 2750 Fragments arranged by size: 2750 1719 1572 722 ApaI G_GGCC'C Cuts at: 2086 3823 2086 Size: 1737 5026 ApaLI G'TGCA_C Cuts at: 2036 4820 5976 2036 Size: 2784 1156 2823 Fragments arranged by size: 2823 2784 1156 ApoI r'AATT_y Cuts at: 1493 3174 3219 3966 4050 4072 4298 4450 6559 Size: 1681 45 747 84 22 226 152 2109 Cuts at: 6559 1493 Size: 1697 Fragments arranged by size: 2109 1697 1681 747 226 152 84 45 22 AseI AT'TA_AT Cuts at: 160 1032 2655 5317 160 Size: 872 1623 2662 1606 Fragments arranged by size: 2662 1623 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6763 AvaI C'yCGr_G Cuts at: 1870 1943 2046 2420 4088 1870 Size: 73 103 374 1668 4545 Fragments arranged by size: 4545 1668 374 103 73 BamHI G'GATC_C Cuts at: 4093 4460 4093 Size: 367 6396 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2230 4084 5539 618 Size: 325 217 627 443 1854 1455 1842 Fragments arranged by size: 1854 1842 1455 627 443 325 217 BanII G_rGCy'C Cuts at: 1391 2086 2876 2960 3128 3449 3785 3823 4082 Size: 695 790 84 168 321 336 38 259 Cuts at: 4082 1391 Size: 4072 Fragments arranged by size: 4072 790 695 336 321 259 168 84 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2770 3921 928 Size: 971 871 1151 3770 Fragments arranged by size: 3770 1151 971 871 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2027 2061 4954 4988 1903 Size: 34 90 34 2893 34 3678 Fragments arranged by size: 3678 2893 90 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4655 5865 4655 Size: 1210 5553 BclI T'GATC_A Cuts at: 1548 1548 Size: 6763 BfrBI ATG'CAT Cuts at: 3154 3154 Size: 6763 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5374 136 Size: 107 122 71 631 605 3702 1525 Fragments arranged by size: 3702 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6758 6758 Size: 6763 BlpI GC'TnA_GC Cuts at: 4180 4180 Size: 6763 Bme1580I G_kGCm'C Cuts at: 1790 2040 2086 3823 4824 5980 1790 Size: 250 46 1737 1001 1156 2573 Fragments arranged by size: 2573 1737 1156 1001 250 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6763 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2916 3084 3168 Size: 416 53 83 399 24 1499 168 84 Cuts at: 3168 3817 4494 5452 442 Size: 649 677 958 1753 Fragments arranged by size: 1753 1499 958 677 649 416 399 168 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6763 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2182 2182 Size: 6763 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2195 3333 3417 3501 3669 5423 1239 Size: 887 69 1138 84 84 168 1754 2579 Fragments arranged by size: 2579 1754 1138 887 168 84 84 69 Bpu10I CC'TnA_GC Cuts at: 2157 2870 2157 Size: 713 6050 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2944 4012 4888 5753 6051 2944 Size: 1068 876 865 298 3656 Fragments arranged by size: 3656 1068 876 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5426 882 Size: 617 3927 2219 Fragments arranged by size: 3927 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4554 493 Size: 711 3350 2702 Fragments arranged by size: 3350 2702 711 BsaBI GATnn'nnATC Cuts at: 6757 6757 Size: 6763 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4570 4952 5587 275 Size: 53 83 186 936 3037 382 635 1451 Fragments arranged by size: 3037 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5196 5594 5868 6015 6426 5196 Size: 398 274 147 411 5533 Fragments arranged by size: 5533 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2045 2075 1178 Size: 30 165 30 642 30 5866 Fragments arranged by size: 5866 642 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2483 2615 1186 Size: 880 417 132 5334 Fragments arranged by size: 5334 880 417 132 BseYI C'CCAG_C Cuts at: 2524 3138 4038 5966 6462 2524 Size: 614 900 1928 496 2825 Fragments arranged by size: 2825 1928 900 614 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2781 3772 1163 Size: 573 1045 991 4154 Fragments arranged by size: 4154 1045 991 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4213 4974 6002 1540 Size: 275 186 2212 761 1028 2301 Fragments arranged by size: 2301 2212 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2040 2876 2960 3128 3617 3785 4824 4909 5980 Size: 836 84 168 489 168 1039 85 1071 Cuts at: 5980 2040 Size: 2823 Fragments arranged by size: 2823 1071 1039 836 489 168 168 85 84 BsiWI C'GTAC_G Cuts at: 2751 2751 Size: 6763 BsmI GAATG_Cn' Cuts at: 2690 3587 3850 4274 4367 2690 Size: 897 263 424 93 5086 Fragments arranged by size: 5086 897 424 263 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2367 2367 Size: 6763 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2086 2876 2960 3128 3449 3617 Size: 399 250 46 790 84 168 321 168 Cuts at: 3617 3785 3823 4082 4824 4909 5980 1391 Size: 168 38 259 742 85 1071 2174 Fragments arranged by size: 2174 1071 790 742 399 321 259 250 168 168 168 85 84 46 38 BspEI T'CCGG_A Cuts at: 6426 6426 Size: 6763 BspHI T'CATG_A Cuts at: 4652 6382 4652 Size: 1730 5033 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2214 4118 844 Size: 1370 1904 3489 Fragments arranged by size: 3489 1904 1370 BsrBI CCG'CTC Cuts at: 1394 1823 4650 1394 Size: 429 2827 3507 Fragments arranged by size: 3507 2827 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4003 5258 5432 65 Size: 1183 2755 1255 174 1396 Fragments arranged by size: 2755 1396 1255 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5407 5594 1700 Size: 110 3597 187 2869 Fragments arranged by size: 3597 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3341 96 Size: 3245 3518 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6763 BssSI C'ACGA_G Cuts at: 4823 5835 4823 Size: 1012 5751 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6763 BstBI TT'CG_AA Cuts at: 5567 5567 Size: 6763 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6763 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6763 BstYI r'GATC_y Cuts at: 1972 3276 4093 4460 4845 4862 6314 6400 6412 Size: 1304 817 367 385 17 1452 86 12 Cuts at: 6412 6758 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1304 817 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2192 2737 513 Size: 552 939 188 545 4539 Fragments arranged by size: 4539 939 552 545 188 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2040 487 Size: 683 56 814 5210 Fragments arranged by size: 5210 814 683 56 BtsI GCAGTG_nn' Cuts at: 2066 2125 3760 4279 5073 5093 2066 Size: 59 1635 519 794 20 3736 Fragments arranged by size: 3736 1635 794 519 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6763 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3085 3120 3985 4020 529 Size: 35 2521 35 865 35 3272 Fragments arranged by size: 3272 2521 865 35 35 35 DraI TTT'AAA Cuts at: 2839 3412 4067 2839 Size: 573 655 5535 Fragments arranged by size: 5535 655 573 DraIII CAC_nnn'GTG Cuts at: 2850 2850 Size: 6763 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5770 809 Size: 507 4454 1802 Fragments arranged by size: 4454 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 4210 5099 Size: 54 1425 50 275 18 244 2136 889 Cuts at: 5099 6728 8 Size: 1629 43 Fragments arranged by size: 2136 1629 1425 889 275 244 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4210 1537 Size: 275 2398 4090 Fragments arranged by size: 4090 2398 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2181 4693 1353 Size: 828 2512 3423 Fragments arranged by size: 3423 2512 828 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2855 5350 5724 5870 387 Size: 1064 138 1266 2495 374 146 1280 Fragments arranged by size: 2495 1280 1266 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2874 2958 3126 3783 2874 Size: 84 168 657 5854 Fragments arranged by size: 5854 657 168 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2195 2613 2764 3016 3100 3333 Size: 393 494 69 418 151 252 84 233 Cuts at: 3333 3417 3501 3505 3589 3669 3673 3757 4826 Size: 84 84 4 84 80 4 84 1069 Cuts at: 4826 5423 6210 6478 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1069 787 597 494 418 393 268 252 233 151 84 84 84 84 84 80 69 4 4 EcoO109I rG'GnC_Cy Cuts at: 2082 2083 2197 2238 3000 3084 3657 3819 2082 Size: 1 114 41 762 84 573 162 5026 Fragments arranged by size: 5026 762 573 162 114 84 41 1 EcoRI G'AATT_C Cuts at: 3219 3966 4072 4450 3219 Size: 747 106 378 5532 Fragments arranged by size: 5532 747 378 106 EcoRV GAT'ATC Cuts at: 2635 2635 Size: 6763 FspI TGC'GCA Cuts at: 5269 5269 Size: 6763 HaeII r_GCGC'y Cuts at: 1678 5910 1678 Size: 4232 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3280 3312 3772 3804 5404 Size: 32 135 32 1708 32 460 32 1600 Cuts at: 5404 5436 5478 5510 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1708 1600 460 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4107 4353 669 Size: 988 281 2169 246 3079 Fragments arranged by size: 3079 2169 988 281 246 HindIII A'AGCT_T Cuts at: 748 2943 3264 4011 748 Size: 2195 321 747 3500 Fragments arranged by size: 3500 2195 747 321 HpaI GTT'AAC Cuts at: 4353 4353 Size: 6763 KpnI G_GTAC'C Cuts at: 4088 4088 Size: 6763 MfeI C'AATT_G Cuts at: 4362 4362 Size: 6763 MluI A'CGCG_T Cuts at: 6639 6639 Size: 6763 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3736 5877 6061 1305 Size: 2431 2141 184 2007 Fragments arranged by size: 2431 2141 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2076 10 Size: 54 1425 343 244 4697 Fragments arranged by size: 4697 1425 343 244 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2118 3244 3412 3866 3909 4721 Size: 648 843 109 1126 168 454 43 812 Cuts at: 4721 5080 5239 518 Size: 359 159 2042 Fragments arranged by size: 2042 1126 843 812 648 454 359 168 159 109 43 MspA1I CmG'CkG Cuts at: 1606 2535 3049 3301 4179 4856 6004 6249 1606 Size: 929 514 252 878 677 1148 245 2120 Fragments arranged by size: 2120 1148 929 878 677 514 252 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2192 2737 513 Size: 552 939 188 545 4539 Fragments arranged by size: 4539 939 552 545 188 NdeI CA'TA_TG Cuts at: 387 387 Size: 6763 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6763 NotI GC'GGCC_GC Cuts at: 4210 4210 Size: 6763 NsiI A_TGCA'T Cuts at: 3156 3156 Size: 6763 NspI r_CATG'y Cuts at: 1132 1229 4121 5666 1132 Size: 97 2892 1545 2229 Fragments arranged by size: 2892 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2509 1348 Size: 1161 5602 PciI A'CATG_T Cuts at: 5662 5662 Size: 6763 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6763 PmeI GTTT'AAAC Cuts at: 4067 4067 Size: 6763 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4832 4864 6345 6377 4832 Size: 32 1481 32 5218 Fragments arranged by size: 5218 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2197 2238 2197 Size: 41 6722 PshAI GACnn'nnGTC Cuts at: 1613 2327 1613 Size: 714 6049 PsiI TTA'TAA Cuts at: 3327 3990 4333 3327 Size: 663 343 5757 Fragments arranged by size: 5757 663 343 PspOMI G'GGCC_C Cuts at: 2082 3819 2082 Size: 1737 5026 PstI C_TGCA'G Cuts at: 830 2211 4115 830 Size: 1381 1904 3478 Fragments arranged by size: 3478 1904 1381 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6763 PvuII CAG'CTG Cuts at: 3049 3301 3049 Size: 252 6511 SacI G_AGCT'C Cuts at: 2876 2960 3128 3785 2876 Size: 84 168 657 5854 Fragments arranged by size: 5854 657 168 84 SalI G'TCGA_C Cuts at: 1936 4105 1936 Size: 2169 4594 SapI GCTCTTCn'nnn_ Cuts at: 2181 2181 Size: 6763 SbfI CC_TGCA'GG Cuts at: 2211 4115 2211 Size: 1904 4859 ScaI AGT'ACT Cuts at: 5011 6723 5011 Size: 1712 5051 SfcI C'TryA_G Cuts at: 826 1045 2089 2207 2920 3228 4111 5246 5927 Size: 219 1044 118 713 308 883 1135 681 Cuts at: 5927 6118 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1044 883 713 681 308 219 191 118 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6763 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6763 SmaI CCC'GGG Cuts at: 1872 2422 4090 1872 Size: 550 1668 4545 Fragments arranged by size: 4545 1668 550 SmlI C'TyrA_G Cuts at: 820 1017 1943 2959 4027 4867 5768 6030 820 Size: 197 926 1016 1068 840 901 262 1553 Fragments arranged by size: 1553 1068 1016 926 901 840 262 197 SnaBI TAC'GTA Cuts at: 493 4554 493 Size: 4061 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6763 SphI G_CATG'C Cuts at: 1132 4121 1132 Size: 2989 3774 SspI AAT'ATT Cuts at: 5 52 4687 5 Size: 47 4635 2081 Fragments arranged by size: 4635 2081 47 StuI AGG'CCT Cuts at: 1517 2282 3113 1517 Size: 765 831 5167 Fragments arranged by size: 5167 831 765 StyI C'CwwG_G Cuts at: 513 1065 2004 2192 2234 2496 2737 4202 513 Size: 552 939 188 42 262 241 1465 3074 Fragments arranged by size: 3074 1465 939 552 262 241 188 42 SwaI ATTT'AAAT Cuts at: 3412 3412 Size: 6763 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4790 4807 4960 5145 5590 325 Size: 1255 3210 17 153 185 445 1498 Fragments arranged by size: 3210 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3341 5009 6721 Size: 274 80 33 51 1443 1364 1668 1712 Cuts at: 6721 96 Size: 138 Fragments arranged by size: 1712 1668 1443 1364 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2045 2077 6723 6755 2045 Size: 32 4646 32 2053 Fragments arranged by size: 4646 2053 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6763 XbaI T'CTAG_A Cuts at: 4099 4099 Size: 6763 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6763 XmaI C'CCGG_G Cuts at: 1870 2420 4088 1870 Size: 550 1668 4545 Fragments arranged by size: 4545 1668 550 XmnI GAAnn'nnTTC Cuts at: 2777 3029 3113 3686 4892 2777 Size: 252 84 573 1206 4648 Fragments arranged by size: 4648 1206 573 252 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4571 276 Size: 53 83 186 936 3037 2468 Fragments arranged by size: 3037 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BstZ17I Bsu36I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI