################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiHKAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 861 CGCG CG^CG AcyI 917 GGCGTC G[GA]^CG[CT]C AfaI 670 GTAC GT^AC AflIII 1292 ACGTGT A^C[GA][CT]GT AocI 796 CCTAAGG CC^T[ACGT]AGG AvaI 508 CTCGAG C^[CT]CG[GA]G BbrPI 1291 CACGTG CAC^GTG BbsI 638 GTCTTC GAAGAC(2/6) BcnI 243 CCGGG CC^[GC]GG BfaI 487 CTAG C^TAG BpuAI 638 GTCTTC GAAGAC(2/6) BsaHI 917 GGCGTC G[GA]^CG[CT]C BsaWI 24 TCCGGT [AT]^CCGG[AT] BseRI 204 GAGGAG GAGGAG(10/8) BsiEI 1204 CGGCCG CG[GA][CT]^CG BsmAI 151 GTCTC GTCTC(1/5) BsmBI 150 CGTCTC CGTCTC(1/5) BsmFI 235 GTCCC GGGAC(10/14) BsmI 327 GCATTC GAATGC(1/-1) BsoBI 508 CTCGAG C^[CT]CG[GA]G BsrBI 550 CCGCTC CCGCTC(-3/-3) BsrDI 1211 CATTGC GCAATG(2/0) BssSI 1120 CTCGTG CACGAG(-5/-1) BstEII 192 GGTGACC G^GT[ACGT]ACC BstPI 192 GGTGACC G^GT[ACGT]ACC BstUI 861 CGCG CG^CG BstYI 86 GGATCT [GA]^GATC[CT] Bsu36I 796 CCTAAGG CC^T[ACGT]AGG DraIII 298 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 226 GACGAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 983 CCATGG C^C[GA][CT]GG EagI 1204 CGGCCG C^GGCCG EclXI 1204 CGGCCG C^GGCCG Eco52I 1204 CGGCCG C^GGCCG Eco81I 796 CCTAAGG CC^T[ACGT]AGG EcoNI 486 CCTAGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 192 GGTGACC G^GT[ACGT]ACC FseI 1201 GGCCGGCC GGCCGG^CC HgaI 918 GCGTC GACGC(5/10) Hin1I 917 GGCGTC G[GA]^CG[CT]C MaeI 487 CTAG C^TAG MflI 86 GGATCT [GA]^GATC[CT] MspA1I 620 CAGCTG C[AC]G^C[GT]G MvnI 861 CGCG CG^CG NciI 243 CCGGG CC^[GC]GG NcoI 983 CCATGG C^CATGG NspI 370 GCATGC [GA]CATG^[CT] PaeR7I 508 CTCGAG C^TCGAG PflMI 36 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1291 CACGTG CAC^GTG PmlI 1291 CACGTG CAC^GTG PvuII 620 CAGCTG CAG^CTG RsaI 670 GTAC GT^AC SfaNI 1283 GCATC GCATC(5/9) SphI 370 GCATGC GCATG^C Van91I 36 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 508 CTCGAG C^TCGAG XhoII 86 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 86 GGATC GGATC(4/5) 688 GGATC BalI 980 TGGCCA TGG^CCA 1229 TGGCCA BpmI 697 CTCCAG CTGGAG(16/14) 201 CTGGAG BsaAI 1162 TACGTG [CT]AC^GT[GA] 1291 CACGTG BstF5I 240 CATCC GGATG(2/0) 1233 CATCC DpnII 87 GATC ^GATC 689 GATC DraII 233 AGGTCCC [GA]G^G[ACGT]CC[CT] 1000 AGGTCCT EcoO109I 233 AGGTCCC [GA]G^G[ACGT]CC[CT] 1000 AGGTCCT EcoT14I 983 CCATGG C^C[AT][AT]GG 1004 CCTTGG FokI 240 CATCC GGATG(9/13) 1233 CATCC HaeII 1062 GGCGCT [GA]GCGC^[CT] 1255 GGCGCT HinfI 736 GAGTC G^A[ACGT]TC 1330 GACTC HphI 192 GGTGA GGTGA(8/7) 742 TCACC MboI 87 GATC ^GATC 689 GATC MluNI 980 TGGCCA TGG^CCA 1229 TGGCCA MscI 980 TGGCCA TGG^CCA 1229 TGGCCA NdeII 87 GATC ^GATC 689 GATC PleI 1330 GACTC GAGTC(4/5) 736 GAGTC PpuMI 233 AGGTCCC [GA]G^G[AT]CC[CT] 1000 AGGTCCT Sau3AI 87 GATC ^GATC 689 GATC StyI 983 CCATGG C^C[AT][AT]GG 1004 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AluI AlwNI AvaII BanI BanII BbvI BglI BmyI BsaJI BsgI BsiYI BslI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI EaeI EarI EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI Ksp632I MaeII MaeIII MboII MnlI MslI MspI MvaI MwoI NaeI NgoMIV NlaIII NlaIV Sau96I ScrFI SmlI StuI TaqI TseI Tsp45I TspRI TthHB8I