(Circular) (Six-base) MAPSORT of: pFN21AE9520.seq Check: 5450 from: 1 to: 6040 pFN21AE9520 6040 bp With 182 enzymes: SgfI * March 28, 2012 12:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2052 2248 2427 2052 Size: 196 179 5665 Fragments arranged by size: 5665 196 179 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2607 4103 5487 5755 1632 Size: 975 1496 1384 268 1917 Fragments arranged by size: 1917 1496 1384 975 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 3297 4939 5916 1203 Size: 2094 1642 977 1327 Fragments arranged by size: 2094 1642 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 2392 1184 Size: 1116 92 4832 Fragments arranged by size: 4832 1116 92 AlwNI CAG_nnn'CTG Cuts at: 2263 2630 2835 3783 5355 2263 Size: 367 205 948 1572 2948 Fragments arranged by size: 2948 1572 948 367 205 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2513 3365 1870 Size: 73 570 852 4545 Fragments arranged by size: 4545 852 570 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2301 2540 2780 3104 3361 Size: 325 217 627 514 239 240 324 257 Cuts at: 3361 4816 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 514 325 324 257 240 239 217 BanII G_rGCy'C Cuts at: 1391 2072 2716 3086 3251 3359 1391 Size: 681 644 370 165 108 4072 Fragments arranged by size: 4072 681 644 370 165 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2636 928 Size: 971 737 4332 Fragments arranged by size: 4332 971 737 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 1548 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 2318 2844 Size: 107 122 71 631 605 616 30 526 Cuts at: 2844 3233 4651 136 Size: 389 1418 1525 Fragments arranged by size: 1525 1418 631 616 605 526 389 122 107 71 30 BglII A'GATC_T Cuts at: 6035 6035 Size: 6040 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2279 2470 2522 2602 3107 4101 5257 1790 Size: 489 191 52 80 505 994 1156 2573 Fragments arranged by size: 2573 1156 994 505 489 191 80 52 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2309 3771 4729 Size: 416 53 83 399 24 892 1462 958 Cuts at: 4729 442 Size: 1753 Fragments arranged by size: 1753 1462 958 892 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2227 2687 4700 1239 Size: 988 460 2013 2579 Fragments arranged by size: 2579 2013 988 460 Bpu10I CC'TnA_GC Cuts at: 3080 3080 Size: 6040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2431 2588 4165 5030 5328 2431 Size: 157 1577 865 298 3143 Fragments arranged by size: 3143 1577 865 298 157 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4703 882 Size: 617 3204 2219 Fragments arranged by size: 3204 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3169 3298 3831 493 Size: 711 1965 129 533 2702 Fragments arranged by size: 2702 1965 711 533 129 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2923 3847 4229 4864 Size: 53 83 186 936 1390 924 382 635 Cuts at: 4864 275 Size: 1451 Fragments arranged by size: 1451 1390 936 924 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2029 4473 4871 5145 5292 5703 2029 Size: 2444 398 274 147 411 2366 Fragments arranged by size: 2444 2366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5815 Fragments arranged by size: 5815 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2224 1186 Size: 1038 5002 BseYI C'CCAG_C Cuts at: 2263 2842 2950 3019 3037 5243 5739 2263 Size: 579 108 69 18 2206 496 2564 Fragments arranged by size: 2564 2206 579 496 108 69 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 2516 2915 3022 3342 1163 Size: 573 540 240 399 107 320 3861 Fragments arranged by size: 3861 573 540 399 320 240 107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3212 3490 4251 5279 1540 Size: 275 186 1211 278 761 1028 2301 Fragments arranged by size: 2301 1211 1028 761 278 275 186 BsiHKAI G_wGCw'C Cuts at: 4101 4186 5257 4101 Size: 85 1071 4884 Fragments arranged by size: 4884 1071 85 BsmI GAATG_Cn' Cuts at: 2332 3551 3644 2332 Size: 1219 93 4728 Fragments arranged by size: 4728 1219 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2161 2161 Size: 6040 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2279 2470 2522 2602 2716 3086 Size: 399 282 207 191 52 80 114 370 Cuts at: 3086 3107 3251 3359 4101 4186 5257 1391 Size: 21 144 108 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 282 207 191 144 114 108 85 80 52 21 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 2227 2248 2427 2994 3395 844 Size: 1208 175 21 179 567 401 3489 Fragments arranged by size: 3489 1208 567 401 179 175 21 BsrBI CCG'CTC Cuts at: 1394 1823 2557 3927 1394 Size: 429 734 1370 3507 Fragments arranged by size: 3507 1370 734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3215 4535 4709 65 Size: 1183 1967 1320 174 1396 Fragments arranged by size: 1967 1396 1320 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3207 3211 3227 4684 4871 1700 Size: 110 1397 4 16 1457 187 2869 Fragments arranged by size: 2869 1457 1397 187 110 16 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6040 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 3125 4100 5112 3125 Size: 975 1012 4053 Fragments arranged by size: 4053 1012 975 BstAPI GCAn_nnn'nTGC Cuts at: 794 2381 2606 2835 794 Size: 1587 225 229 3999 Fragments arranged by size: 3999 1587 229 225 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 2197 1184 Size: 1013 5027 BstXI CCAn_nnnn'nTGG Cuts at: 2136 2251 2971 3244 2136 Size: 115 720 273 4932 Fragments arranged by size: 4932 720 273 115 BstYI r'GATC_y Cuts at: 1972 2091 3370 3737 4122 4139 5591 5677 5689 Size: 119 1279 367 385 17 1452 86 12 Cuts at: 5689 6035 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1279 385 367 346 119 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2802 2802 Size: 6040 BtgI C'CryG_G Cuts at: 513 1065 2004 2988 513 Size: 552 939 984 3565 Fragments arranged by size: 3565 984 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 2750 2836 2962 3556 4350 4370 2750 Size: 86 126 594 794 20 4420 Fragments arranged by size: 4420 794 594 126 86 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 3344 3344 Size: 6040 DraIII CAC_nnn'GTG Cuts at: 2308 2308 Size: 6040 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2237 5047 809 Size: 507 921 2810 1802 Fragments arranged by size: 2810 1802 921 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2853 2985 3209 Size: 54 1425 50 275 18 1023 132 224 Cuts at: 3209 3234 3487 4376 6005 8 Size: 25 253 889 1629 43 Fragments arranged by size: 1629 1425 1023 889 275 253 224 132 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3209 3487 1537 Size: 275 1397 278 4090 Fragments arranged by size: 4090 1397 278 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2724 2733 3970 1353 Size: 710 661 9 1237 3423 Fragments arranged by size: 3423 1237 710 661 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2227 2607 2687 4103 4700 5487 5755 Size: 393 595 380 80 1416 597 787 268 Cuts at: 5755 1239 Size: 1524 Fragments arranged by size: 1524 1416 787 597 595 393 380 268 80 EcoNI CCTnn'n_nnAGG Cuts at: 2495 2495 Size: 6040 EcoO109I rG'GnC_Cy Cuts at: 2239 3006 2239 Size: 767 5273 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FseI GG_CCGG'CC Cuts at: 3211 3211 Size: 6040 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 3071 3264 5187 1678 Size: 1393 193 1923 2531 Fragments arranged by size: 2531 1923 1393 193 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2678 2685 2710 2717 4681 Size: 32 135 32 1106 7 25 7 1964 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1964 1106 135 42 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 3384 3630 669 Size: 988 281 1446 246 3079 Fragments arranged by size: 3079 1446 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 3639 3639 Size: 6040 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2117 5154 5338 1305 Size: 812 3037 184 2007 Fragments arranged by size: 3037 2007 812 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2987 3236 10 Size: 54 1425 343 1155 249 2814 Fragments arranged by size: 2814 1425 1155 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 2374 2993 3242 3998 4357 4516 Size: 648 1083 125 619 249 756 359 159 Cuts at: 4516 518 Size: 2042 Fragments arranged by size: 2042 1083 756 648 619 359 249 159 125 MspA1I CmG'CkG Cuts at: 1606 2627 3456 4133 5281 5526 1606 Size: 1021 829 677 1148 245 2120 Fragments arranged by size: 2120 1148 1021 829 677 245 NaeI GCC'GGC Cuts at: 3209 3213 3229 3209 Size: 4 16 6020 Fragments arranged by size: 6020 16 4 NcoI C'CATG_G Cuts at: 513 1065 2004 2988 513 Size: 552 939 984 3565 Fragments arranged by size: 3565 984 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NgoMIV G'CCGG_C Cuts at: 3207 3211 3227 3207 Size: 4 16 6020 Fragments arranged by size: 6020 16 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 2379 3398 4943 1132 Size: 97 1150 1019 1545 2229 Fragments arranged by size: 2229 1545 1150 1019 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 1108 Size: 939 5101 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PmlI CAC'GTG Cuts at: 3298 3298 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2239 3006 2239 Size: 767 5273 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PspXI vC'TCGA_Gb Cuts at: 2513 2513 Size: 6040 PstI C_TGCA'G Cuts at: 830 3392 830 Size: 2562 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 2627 2627 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 3388 4523 5204 5395 826 Size: 219 2343 1135 681 191 1471 Fragments arranged by size: 2343 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2410 2513 2567 4144 5045 5307 Size: 197 926 467 103 54 1577 901 262 Cuts at: 5307 820 Size: 1553 Fragments arranged by size: 1577 1553 926 901 467 262 197 103 54 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 2379 3398 1132 Size: 1247 1019 3774 Fragments arranged by size: 3774 1247 1019 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 2460 2700 2807 1517 Size: 943 240 107 4750 Fragments arranged by size: 4750 943 240 107 StyI C'CwwG_G Cuts at: 513 1065 2004 2988 3009 3479 513 Size: 552 939 984 21 470 3074 Fragments arranged by size: 3074 984 939 552 470 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2029 2086 2981 3130 4067 4084 4237 Size: 1255 449 57 895 149 937 17 153 Cuts at: 4237 4422 4867 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 937 895 449 445 185 153 149 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4286 5998 96 Size: 274 80 33 51 1443 2309 1712 138 Fragments arranged by size: 2309 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2776 2808 6000 6032 2776 Size: 32 3192 32 2784 Fragments arranged by size: 3192 2784 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 2513 1943 Size: 570 5470 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 4169 4169 Size: 6040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI