################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AvrII BamHI BbrPI BbsI BcgI BciVI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I HincII HindII HindIII HpaI Ksp632I MamI MfeI MluI MroI MunI NdeI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 107 GGTACC G^GTACC AcyI 90 GGCGCC G[GA]^CG[CT]C AflII 1392 CTTAAG C^TTAAG AocI 1437 CCTCAGG CC^T[ACGT]AGG Asp718I 107 GGTACC G^GTACC AspI 480 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 821 TGCGCA TGC^GCA BalI 1344 TGGCCA TGG^CCA BbeI 90 GGCGCC GGCGC^C BbvCI 982 CCTCAGC CCTCAGC(-5/-2) BclI 1478 TGATCA T^GATCA BfrI 1392 CTTAAG C^TTAAG BglI 1247 GCCTGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 90 GGCGCC G[GA]^CG[CT]C BsaI 335 GAGACC GGTCTC(1/5) BsgI 361 GTGCAG GTGCAG(16/14) BsrBI 57 CCGCTC CCGCTC(-3/-3) BstAPI 881 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 779 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1437 CCTCAGG CC^T[ACGT]AGG Eco81I 1437 CCTCAGG CC^T[ACGT]AGG EcoNI 1368 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1478 TGATCA T^GATCA FseI 502 GGCCGGCC GGCCGG^CC FspI 821 TGCGCA TGC^GCA Hin1I 90 GGCGCC G[GA]^CG[CT]C KasI 90 GGCGCC G^GCGCC KpnI 107 GGTACC GGTAC^C MaeII 1258 ACGT A^CGT MluNI 1344 TGGCCA TGG^CCA MscI 1344 TGGCCA TGG^CCA MslI 780 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 90 GGCGCC GG^CGCC NcoI 767 CCATGG C^CATGG NheI 720 GCTAGC G^CTAGC NotI 268 GCGGCCGC GC^GGCCGC NspI 131 GCATGC [GA]CATG^[CT] PflFI 480 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC SfoI 90 GGCGCC GGC^GCC SphI 131 GCATGC GCATG^C TaqI 959 TCGA T^CGA Tth111I 480 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 959 TCGA T^CGA XcmI 398 CCATGCCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 108 GTAC GT^AC 622 GTAC AlwI 1167 GGATC GGATC(4/5) 1209 GGATC ApaI 219 GGGCCC GGGCC^C 316 GGGCCC BseRI 802 GAGGAG GAGGAG(10/8) 1075 GAGGAG BsmAI 335 GAGAC GTCTC(1/5) 615 GTCTC BstF5I 603 CATCC GGATG(2/0) 861 CATCC BstYI 1167 GGATCT [GA]^GATC[CT] 1209 GGATCT DrdI 480 GACCCTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 706 GACGCTCTGGTC FokI 603 CATCC GGATG(9/13) 861 CATCC KspI 49 CCGCGG CCGC^GG 230 CCGCGG MboII 534 GAAGA GAAGA(8/7) 1170 TCTTC MflI 1167 GGATCT [GA]^GATC[CT] 1209 GGATCT MseI 1393 TTAA T^TAA 1433 TTAA NaeI 503 GCCGGC GCC^GGC 1194 GCCGGC NgoMIV 503 GCCGGC G^CCGGC 1194 GCCGGC PflMI 744 CCATAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 900 CCAGGTCCTGG PstI 118 CTGCAG CTGCA^G 967 CTGCAG RsaI 108 GTAC GT^AC 622 GTAC SacI 1078 GAGCTC GAGCT^C 1092 GAGCTC SacII 49 CCGCGG CCGC^GG 230 CCGCGG SfaNI 923 GCATC GCATC(5/9) 1187 GCATC Tru9I 1393 TTAA T^TAA 1433 TTAA Van91I 744 CCATAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 900 CCAGGTCCTGG XhoII 1167 GGATCT [GA]^GATC[CT] 1209 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AluI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiEI BsiHKAI BsiYI BslI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII Sau3AI Sau96I ScrFI SfcI SmaI SmlI StyI TseI Tsp45I TspRI XmaCI XmaI