(Circular) (Six-base) MAPSORT of: pFN21AE3231.seq Check: 4412 from: 1 to: 6322 pFN21AE3231 6322 bp With 182 enzymes: SgfI * March 28, 2012 12:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2127 2127 Size: 6322 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4132 278 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3665 1937 Size: 1728 4594 Acc65I G'GTAC_C Cuts at: 2112 3643 2112 Size: 1531 4791 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2936 3194 3569 4385 5769 6037 1632 Size: 1304 258 375 816 1384 268 1917 Fragments arranged by size: 1917 1384 1304 816 375 268 258 AflII C'TTAA_G Cuts at: 820 1017 3397 820 Size: 197 2380 3745 Fragments arranged by size: 3745 2380 197 AflIII A'CryG_T Cuts at: 1203 5221 6198 1203 Size: 4018 977 1327 Fragments arranged by size: 4018 1327 977 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 2789 1166 Size: 1623 4699 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6322 AlwNI CAG_nnn'CTG Cuts at: 2599 2892 2911 3001 3217 3385 3492 4065 5637 Size: 293 19 90 216 168 107 573 1572 Cuts at: 5637 2599 Size: 3284 Fragments arranged by size: 3284 1572 573 293 216 168 107 90 19 ApaI G_GGCC'C Cuts at: 2228 2325 2228 Size: 97 6225 ApaLI G'TGCA_C Cuts at: 4379 5535 4379 Size: 1156 5166 ApoI r'AATT_y Cuts at: 1493 3631 3857 4009 6118 1493 Size: 2138 226 152 2109 1697 Fragments arranged by size: 2138 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 2043 2107 2220 2312 3453 3496 3540 Size: 73 100 64 113 92 1141 43 44 Cuts at: 3540 3647 1870 Size: 107 4545 Fragments arranged by size: 4545 1141 113 107 100 92 73 64 44 43 BamHI G'GATC_C Cuts at: 3652 4019 3652 Size: 367 5955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2095 2112 2296 3318 3449 Size: 325 217 627 308 17 184 1022 131 Cuts at: 3449 3643 5098 618 Size: 194 1455 1842 Fragments arranged by size: 1842 1455 1022 627 325 308 217 194 184 131 17 BanII G_rGCy'C Cuts at: 1391 2044 2108 2228 2259 2325 3087 3101 3416 Size: 653 64 120 31 66 762 14 315 Cuts at: 3416 3641 1391 Size: 225 4072 Fragments arranged by size: 4072 762 653 315 225 120 66 64 31 14 BbeI G_GCGC'C Cuts at: 2099 2099 Size: 6322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5351 BbvCI CC'TCA_GC Cuts at: 2988 2988 Size: 6322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4513 4547 1903 Size: 34 2576 34 3678 Fragments arranged by size: 3678 2576 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4214 5424 4214 Size: 1210 5112 BclI T'GATC_A Cuts at: 1548 3483 1548 Size: 1935 4387 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3258 4933 136 Size: 107 122 71 631 605 1586 1675 1525 Fragments arranged by size: 1675 1586 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6317 6317 Size: 6322 BlpI GC'TnA_GC Cuts at: 3739 3739 Size: 6322 Bme1580I G_kGCm'C Cuts at: 1790 2228 2325 3452 4383 5539 1790 Size: 438 97 1127 931 1156 2573 Fragments arranged by size: 2573 1156 1127 931 438 97 BmgBI CAC'GTC Cuts at: 1318 3264 1318 Size: 1946 4376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4053 5011 442 Size: 416 53 83 399 24 2636 958 1753 Fragments arranged by size: 2636 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2729 1056 Size: 1673 4649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2576 2579 2888 2933 2978 3254 3395 3539 Size: 1337 3 309 45 45 276 141 144 Cuts at: 3539 3590 3611 4982 1239 Size: 51 21 1371 2579 Fragments arranged by size: 2579 1371 1337 309 276 144 141 51 45 45 21 3 Bpu10I CC'TnA_GC Cuts at: 2258 2988 2258 Size: 730 5592 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2663 3364 4447 5312 5610 2663 Size: 701 1083 865 298 3375 Fragments arranged by size: 3375 1083 865 701 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2334 4985 882 Size: 617 835 2651 2219 Fragments arranged by size: 2651 2219 835 617 BsaAI yAC'GTr Cuts at: 493 1204 4113 493 Size: 711 2909 2702 Fragments arranged by size: 2909 2702 711 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2096 4129 4511 5146 Size: 53 83 186 936 563 2033 382 635 Cuts at: 5146 275 Size: 1451 Fragments arranged by size: 2033 1451 936 635 563 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4755 5153 5427 5574 5985 4755 Size: 398 274 147 411 5092 Fragments arranged by size: 5092 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2801 2831 1178 Size: 30 165 30 1398 30 4669 Fragments arranged by size: 4669 1398 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2822 3095 1186 Size: 1636 273 4413 Fragments arranged by size: 4413 1636 273 BseYI C'CCAG_C Cuts at: 2014 2228 2334 2881 2898 3421 3617 5525 6021 Size: 214 106 547 17 523 196 1908 496 Cuts at: 6021 2014 Size: 2315 Fragments arranged by size: 2315 1908 547 523 496 214 196 106 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2387 1163 Size: 573 651 5098 Fragments arranged by size: 5098 651 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 2277 2509 3772 4533 5561 Size: 275 186 69 207 232 1263 761 1028 Cuts at: 5561 1540 Size: 2301 Fragments arranged by size: 2301 1263 1028 761 275 232 207 186 69 BsiHKAI G_wGCw'C Cuts at: 2924 3087 3101 3434 4383 4468 5539 2924 Size: 163 14 333 949 85 1071 3707 Fragments arranged by size: 3707 1071 949 333 163 85 14 BsmI GAATG_Cn' Cuts at: 2346 2829 2943 3833 3926 2346 Size: 483 114 890 93 4742 Fragments arranged by size: 4742 890 483 114 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2044 2108 2228 2259 2325 2924 3087 Size: 399 254 64 120 31 66 599 163 Cuts at: 3087 3101 3416 3434 3452 3641 4383 4468 5539 Size: 14 315 18 18 189 742 85 1071 Cuts at: 5539 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 599 399 315 254 189 163 120 85 66 64 31 18 18 14 BspEI T'CCGG_A Cuts at: 5985 5985 Size: 6322 BspHI T'CATG_A Cuts at: 4211 5941 4211 Size: 1730 4592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2017 2127 3113 3168 3677 844 Size: 1173 110 986 55 509 3489 Fragments arranged by size: 3489 1173 986 509 110 55 BsrBI CCG'CTC Cuts at: 1394 1823 2064 4209 1394 Size: 429 241 2145 3507 Fragments arranged by size: 3507 2145 429 241 BsrDI GCAATG_nn' Cuts at: 65 1248 4817 4991 65 Size: 1183 3569 174 1396 Fragments arranged by size: 3569 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2088 2293 2508 2924 3199 3270 4966 Size: 110 278 205 215 416 275 71 1696 Cuts at: 4966 5153 1700 Size: 187 2869 Fragments arranged by size: 2869 1696 416 278 275 215 205 187 110 71 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6322 BssHII G'CGCG_C Cuts at: 1925 2188 2426 3188 1925 Size: 263 238 762 5059 Fragments arranged by size: 5059 762 263 238 BssSI C'ACGA_G Cuts at: 4382 5394 4382 Size: 1012 5310 BstAPI GCAn_nnn'nTGC Cuts at: 794 2892 794 Size: 2098 4224 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6322 BstXI CCAn_nnnn'nTGG Cuts at: 2791 2791 Size: 6322 BstYI r'GATC_y Cuts at: 1972 3172 3214 3652 4019 4404 4421 5873 5959 Size: 1200 42 438 367 385 17 1452 86 Cuts at: 5959 5971 6317 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1200 438 385 367 346 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 3443 3443 Size: 6322 BtgI C'CryG_G Cuts at: 513 1065 2004 2054 2235 2695 2772 3145 513 Size: 552 939 50 181 460 77 373 3690 Fragments arranged by size: 3690 939 552 460 373 181 77 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5583 Fragments arranged by size: 5583 683 56 BtsI GCAGTG_nn' Cuts at: 2876 3326 3838 4632 4652 2876 Size: 450 512 794 20 4546 Fragments arranged by size: 4546 794 512 450 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6322 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 3626 3626 Size: 6322 DraIII CAC_nnn'GTG Cuts at: 2482 2635 2701 2482 Size: 153 66 6103 Fragments arranged by size: 6103 153 66 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2491 2717 5329 809 Size: 507 1175 226 2612 1802 Fragments arranged by size: 2612 1802 1175 507 226 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2238 2274 Size: 54 1425 50 275 18 237 171 36 Cuts at: 2274 2506 3349 3769 4658 6287 8 Size: 232 843 420 889 1629 43 Fragments arranged by size: 1629 1425 889 843 420 275 237 232 171 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 2067 2274 2506 3769 1537 Size: 275 255 207 232 1263 4090 Fragments arranged by size: 4090 1263 275 255 232 207 EarI CTCTTCn'nnn_ Cuts at: 1353 4252 1353 Size: 2899 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2408 2549 4909 5283 5429 387 Size: 1064 138 819 141 2360 374 146 1280 Fragments arranged by size: 2360 1280 1064 819 374 146 141 138 EcoICRI GAG'CTC Cuts at: 3085 3099 3085 Size: 14 6308 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2576 2579 2888 2933 2936 2978 3194 Size: 393 944 3 309 45 3 42 216 Cuts at: 3194 3254 3395 3539 3569 3590 3611 4385 4982 Size: 60 141 144 30 21 21 774 597 Cuts at: 4982 5769 6037 1239 Size: 787 268 1524 Fragments arranged by size: 1524 944 787 774 597 393 309 268 216 144 141 60 45 42 30 21 21 3 3 EcoNI CCTnn'n_nnAGG Cuts at: 3377 3377 Size: 6322 EcoO109I rG'GnC_Cy Cuts at: 2224 2225 2321 2817 2908 3127 3370 3382 3565 Size: 1 96 496 91 219 243 12 183 Cuts at: 3565 2224 Size: 4981 Fragments arranged by size: 4981 496 243 219 183 96 91 12 1 EcoRI G'AATT_C Cuts at: 3631 4009 3631 Size: 378 5944 FseI GG_CCGG'CC Cuts at: 2512 2512 Size: 6322 FspI TGC'GCA Cuts at: 2828 4828 2828 Size: 2000 4322 HaeII r_GCGC'y Cuts at: 1678 2054 2099 2235 5469 1678 Size: 376 45 136 3234 2531 Fragments arranged by size: 3234 2531 376 136 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2801 2833 4963 4995 5037 Size: 32 135 32 1229 32 2130 32 42 Cuts at: 5037 5069 1373 Size: 32 2626 Fragments arranged by size: 2626 2130 1229 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3912 669 Size: 988 281 1728 246 3079 Fragments arranged by size: 3079 1728 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6322 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KasI G'GCGC_C Cuts at: 2095 2095 Size: 6322 KpnI G_GTAC'C Cuts at: 2116 3647 2116 Size: 1531 4791 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2056 2154 5436 5620 1305 Size: 751 98 3282 184 2007 Fragments arranged by size: 3282 2007 751 184 98 MscI TGG'CCA Cuts at: 10 64 1489 1832 3351 10 Size: 54 1425 343 1519 2981 Fragments arranged by size: 2981 1519 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 4280 4639 4798 518 Size: 648 1623 1491 359 159 2042 Fragments arranged by size: 2042 1623 1491 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2185 2237 2442 2500 2789 2889 2956 Size: 450 129 52 205 58 289 100 67 Cuts at: 2956 3492 3531 3598 3738 4415 5563 5808 1606 Size: 536 39 67 140 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 536 450 289 245 205 140 129 100 67 67 58 52 39 NaeI GCC'GGC Cuts at: 2510 3201 2510 Size: 691 5631 NarI GG'CG_CC Cuts at: 2096 2096 Size: 6322 NcoI C'CATG_G Cuts at: 513 1065 2004 2772 513 Size: 552 939 768 4063 Fragments arranged by size: 4063 939 768 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NgoMIV G'CCGG_C Cuts at: 2508 3199 2508 Size: 691 5631 NheI G'CTAG_C Cuts at: 1052 2725 1052 Size: 1673 4649 NotI GC'GGCC_GC Cuts at: 2274 3769 2274 Size: 1495 4827 NspI r_CATG'y Cuts at: 1132 1229 2140 3680 5225 1132 Size: 97 911 1540 1545 2229 Fragments arranged by size: 2229 1545 1540 911 97 PasI CC'CwG_GG Cuts at: 1348 3407 1348 Size: 2059 4263 PciI A'CATG_T Cuts at: 5221 5221 Size: 6322 PflMI CCAn_nnn'nTGG Cuts at: 1108 2755 2911 1108 Size: 1647 156 4519 Fragments arranged by size: 4519 1647 156 PfoI T'CCnGG_A Cuts at: 2910 3037 3055 2910 Size: 127 18 6177 Fragments arranged by size: 6177 127 18 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2817 2908 3127 3370 2817 Size: 91 219 243 5769 Fragments arranged by size: 5769 243 219 91 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PspOMI G'GGCC_C Cuts at: 2224 2321 2224 Size: 97 6225 PstI C_TGCA'G Cuts at: 830 2127 2976 3674 830 Size: 1297 849 698 3478 Fragments arranged by size: 3478 1297 849 698 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 PvuII CAG'CTG Cuts at: 2789 2889 2956 3492 3598 2789 Size: 100 67 536 106 5513 Fragments arranged by size: 5513 536 106 100 67 SacI G_AGCT'C Cuts at: 3087 3101 3087 Size: 14 6308 SacII CC_GC'GG Cuts at: 2057 2238 2057 Size: 181 6141 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SanDI GG'GwC_CC Cuts at: 2817 2817 Size: 6322 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SfcI C'TryA_G Cuts at: 826 1045 2123 2762 2845 2972 3670 4805 5486 Size: 219 1078 639 83 127 698 1135 681 Cuts at: 5486 5677 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1078 698 681 639 219 191 127 83 SfoI GGC'GCC Cuts at: 2097 2097 Size: 6322 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 2045 2222 3455 3498 3542 3649 1872 Size: 173 177 1233 43 44 107 4545 Fragments arranged by size: 4545 1233 177 173 107 44 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 3343 3397 4426 5327 5589 Size: 197 926 735 665 54 1029 901 262 Cuts at: 5589 820 Size: 1553 Fragments arranged by size: 1553 1029 926 901 735 665 262 197 54 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 2140 3680 1132 Size: 1008 1540 3774 Fragments arranged by size: 3774 1540 1008 SrfI GCCC'GGGC Cuts at: 2045 3498 2045 Size: 1453 4869 SspI AAT'ATT Cuts at: 5 52 4246 5 Size: 47 4194 2081 Fragments arranged by size: 4194 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6322 StyI C'CwwG_G Cuts at: 513 1065 2004 2247 2352 2517 2772 3285 3761 Size: 552 939 243 105 165 255 513 476 Cuts at: 3761 513 Size: 3074 Fragments arranged by size: 3074 939 552 513 476 255 243 165 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2314 3435 3631 4349 4366 4519 4704 Size: 1255 734 1121 196 718 17 153 185 Cuts at: 4704 5149 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1121 734 718 445 196 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4568 6280 96 Size: 274 80 33 51 1443 2591 1712 138 Fragments arranged by size: 2591 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2066 2098 3033 3065 3566 3598 6282 6314 2066 Size: 32 935 32 501 32 2684 32 2074 Fragments arranged by size: 2684 2074 935 501 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2488 1316 Size: 1172 5150 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2410 2410 Size: 6322 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6322 XmaI C'CCGG_G Cuts at: 1870 2043 2220 3453 3496 3540 3647 1870 Size: 173 177 1233 43 44 107 4545 Fragments arranged by size: 4545 1233 177 173 107 44 43 XmnI GAAnn'nnTTC Cuts at: 4451 4451 Size: 6322 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4130 276 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI SwaI