(Linear) (Six-base) MAPSORT of: KIEE3231.seq Check: 2046 from: 1 to: 1617 KIEE3231.orf With 182 enzymes: SgfI * March 22, 2012 16:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 122 1617 Size: 122 1495 Acc65I G'GTAC_C Cuts at: 0 107 1617 Size: 107 1510 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 931 1189 1564 1617 Size: 931 258 375 53 Fragments arranged by size: 931 375 258 53 AflII C'TTAA_G Cuts at: 0 1392 1617 Size: 1392 225 AleI CACnn'nnGTG Cuts at: 0 784 1617 Size: 784 833 AlwNI CAG_nnn'CTG Cuts at: 0 594 887 906 996 1212 1380 1487 1617 Size: 594 293 19 90 216 168 107 130 Fragments arranged by size: 594 293 216 168 130 107 90 19 ApaI G_GGCC'C Cuts at: 0 223 320 1617 Size: 223 97 1297 Fragments arranged by size: 1297 223 97 AvaI C'yCGr_G Cuts at: 0 38 102 215 307 1448 1491 1535 1617 Size: 38 64 113 92 1141 43 44 82 Fragments arranged by size: 1141 113 92 82 64 44 43 38 BanI G'GyrC_C Cuts at: 0 90 107 291 1313 1444 1617 Size: 90 17 184 1022 131 173 Fragments arranged by size: 1022 184 173 131 90 17 BanII G_rGCy'C Cuts at: 0 39 103 223 254 320 1082 1096 1411 Size: 39 64 120 31 66 762 14 315 Cuts at: 1411 1617 Size: 206 Fragments arranged by size: 762 315 206 120 66 64 39 31 14 BbeI G_GCGC'C Cuts at: 0 94 1617 Size: 94 1523 BbvCI CC'TCA_GC Cuts at: 0 983 1617 Size: 983 634 BclI T'GATC_A Cuts at: 0 1478 1617 Size: 1478 139 BglI GCCn_nnn'nGGC Cuts at: 0 1253 1617 Size: 1253 364 Bme1580I G_kGCm'C Cuts at: 0 223 320 1447 1617 Size: 223 97 1127 170 Fragments arranged by size: 1127 223 170 97 BmgBI CAC'GTC Cuts at: 0 1259 1617 Size: 1259 358 BmtI G_CTAG'C Cuts at: 0 724 1617 Size: 724 893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 574 883 928 973 1249 1390 1534 Size: 571 3 309 45 45 276 141 144 Cuts at: 1534 1585 1606 1617 Size: 51 21 11 Fragments arranged by size: 571 309 276 144 141 51 45 45 21 11 3 Bpu10I CC'TnA_GC Cuts at: 0 253 983 1617 Size: 253 730 634 Fragments arranged by size: 730 634 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 658 1359 1617 Size: 658 701 258 Fragments arranged by size: 701 658 258 BsaI GGTCTCn'nnnn_ Cuts at: 0 329 1617 Size: 329 1288 BsaHI Gr'CG_yC Cuts at: 0 91 1617 Size: 91 1526 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 796 826 1617 Size: 796 30 791 Fragments arranged by size: 796 791 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 817 1090 1617 Size: 817 273 527 Fragments arranged by size: 817 527 273 BseYI C'CCAG_C Cuts at: 0 9 223 329 876 893 1416 1612 1617 Size: 9 214 106 547 17 523 196 5 Fragments arranged by size: 547 523 214 196 106 17 9 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 1617 Size: 382 1235 BsiEI CG_ry'CG Cuts at: 0 65 272 504 1617 Size: 65 207 232 1113 Fragments arranged by size: 1113 232 207 65 BsiHKAI G_wGCw'C Cuts at: 0 919 1082 1096 1429 1617 Size: 919 163 14 333 188 Fragments arranged by size: 919 333 188 163 14 BsmI GAATG_Cn' Cuts at: 0 341 824 938 1617 Size: 341 483 114 679 Fragments arranged by size: 679 483 341 114 Bsp1286I G_dGCh'C Cuts at: 0 39 103 223 254 320 919 1082 1096 Size: 39 64 120 31 66 599 163 14 Cuts at: 1096 1411 1429 1447 1617 Size: 315 18 18 170 Fragments arranged by size: 599 315 170 163 120 66 64 39 31 18 18 14 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 12 122 1108 1163 1617 Size: 12 110 986 55 454 Fragments arranged by size: 986 454 110 55 12 BsrBI CCG'CTC Cuts at: 0 59 1617 Size: 59 1558 BsrFI r'CCGG_y Cuts at: 0 83 288 503 919 1194 1265 1617 Size: 83 205 215 416 275 71 352 Fragments arranged by size: 416 352 275 215 205 83 71 BssHII G'CGCG_C Cuts at: 0 183 421 1183 1617 Size: 183 238 762 434 Fragments arranged by size: 762 434 238 183 BstAPI GCAn_nnn'nTGC Cuts at: 0 887 1617 Size: 887 730 BstXI CCAn_nnnn'nTGG Cuts at: 0 786 1617 Size: 786 831 BstYI r'GATC_y Cuts at: 0 1167 1209 1617 Size: 1167 42 408 Fragments arranged by size: 1167 408 42 Bsu36I CC'TnA_GG Cuts at: 0 1438 1617 Size: 1438 179 BtgI C'CryG_G Cuts at: 0 49 230 690 767 1140 1617 Size: 49 181 460 77 373 477 Fragments arranged by size: 477 460 373 181 77 49 BtsI GCAGTG_nn' Cuts at: 0 871 1321 1617 Size: 871 450 296 Fragments arranged by size: 871 450 296 DraIII CAC_nnn'GTG Cuts at: 0 477 630 696 1617 Size: 477 153 66 921 Fragments arranged by size: 921 477 153 66 DrdI GACnn_nn'nnGTC Cuts at: 0 486 712 1617 Size: 486 226 905 Fragments arranged by size: 905 486 226 EaeI y'GGCC_r Cuts at: 0 62 233 269 501 1344 1617 Size: 62 171 36 232 843 273 Fragments arranged by size: 843 273 232 171 62 36 EagI C'GGCC_G Cuts at: 0 62 269 501 1617 Size: 62 207 232 1116 Fragments arranged by size: 1116 232 207 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 403 544 1617 Size: 403 141 1073 Fragments arranged by size: 1073 403 141 EcoICRI GAG'CTC Cuts at: 0 1080 1094 1617 Size: 1080 14 523 Fragments arranged by size: 1080 523 14 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 574 883 928 931 973 1189 1249 Size: 571 3 309 45 3 42 216 60 Cuts at: 1249 1390 1534 1564 1585 1606 1617 Size: 141 144 30 21 21 11 Fragments arranged by size: 571 309 216 144 141 60 45 42 30 21 21 11 3 3 EcoNI CCTnn'n_nnAGG Cuts at: 0 1372 1617 Size: 1372 245 EcoO109I rG'GnC_Cy Cuts at: 0 219 220 316 812 903 1122 1365 1377 Size: 219 1 96 496 91 219 243 12 Cuts at: 1377 1560 1617 Size: 183 57 Fragments arranged by size: 496 243 219 219 183 96 91 57 12 1 FseI GG_CCGG'CC Cuts at: 0 507 1617 Size: 507 1110 FspI TGC'GCA Cuts at: 0 823 1617 Size: 823 794 HaeII r_GCGC'y Cuts at: 0 49 94 230 1617 Size: 49 45 136 1387 Fragments arranged by size: 1387 136 49 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 796 828 1617 Size: 796 32 789 Fragments arranged by size: 796 789 32 KasI G'GCGC_C Cuts at: 0 90 1617 Size: 90 1527 KpnI G_GTAC'C Cuts at: 0 111 1617 Size: 111 1506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 51 149 1617 Size: 51 98 1468 Fragments arranged by size: 1468 98 51 MscI TGG'CCA Cuts at: 0 1346 1617 Size: 1346 271 MslI CAynn'nnrTG Cuts at: 0 784 1617 Size: 784 833 MspA1I CmG'CkG Cuts at: 0 51 180 232 437 495 784 884 951 Size: 51 129 52 205 58 289 100 67 Cuts at: 951 1487 1526 1593 1617 Size: 536 39 67 24 Fragments arranged by size: 536 289 205 129 100 67 67 58 52 51 39 24 NaeI GCC'GGC Cuts at: 0 505 1196 1617 Size: 505 691 421 Fragments arranged by size: 691 505 421 NarI GG'CG_CC Cuts at: 0 91 1617 Size: 91 1526 NcoI C'CATG_G Cuts at: 0 767 1617 Size: 767 850 NgoMIV G'CCGG_C Cuts at: 0 503 1194 1617 Size: 503 691 423 Fragments arranged by size: 691 503 423 NheI G'CTAG_C Cuts at: 0 720 1617 Size: 720 897 NotI GC'GGCC_GC Cuts at: 0 269 1617 Size: 269 1348 NspI r_CATG'y Cuts at: 0 135 1617 Size: 135 1482 PasI CC'CwG_GG Cuts at: 0 1402 1617 Size: 1402 215 PflMI CCAn_nnn'nTGG Cuts at: 0 750 906 1617 Size: 750 156 711 Fragments arranged by size: 750 711 156 PfoI T'CCnGG_A Cuts at: 0 905 1032 1050 1617 Size: 905 127 18 567 Fragments arranged by size: 905 567 127 18 PpuMI rG'GwC_Cy Cuts at: 0 812 903 1122 1365 1617 Size: 812 91 219 243 252 Fragments arranged by size: 812 252 243 219 91 PspOMI G'GGCC_C Cuts at: 0 219 316 1617 Size: 219 97 1301 Fragments arranged by size: 1301 219 97 PstI C_TGCA'G Cuts at: 0 122 971 1617 Size: 122 849 646 Fragments arranged by size: 849 646 122 PvuII CAG'CTG Cuts at: 0 784 884 951 1487 1593 1617 Size: 784 100 67 536 106 24 Fragments arranged by size: 784 536 106 100 67 24 SacI G_AGCT'C Cuts at: 0 1082 1096 1617 Size: 1082 14 521 Fragments arranged by size: 1082 521 14 SacII CC_GC'GG Cuts at: 0 52 233 1617 Size: 52 181 1384 Fragments arranged by size: 1384 181 52 SanDI GG'GwC_CC Cuts at: 0 812 1617 Size: 812 805 SfcI C'TryA_G Cuts at: 0 118 757 840 967 1617 Size: 118 639 83 127 650 Fragments arranged by size: 650 639 127 118 83 SfoI GGC'GCC Cuts at: 0 92 1617 Size: 92 1525 SmaI CCC'GGG Cuts at: 0 40 217 1450 1493 1537 1617 Size: 40 177 1233 43 44 80 Fragments arranged by size: 1233 177 80 44 43 40 SmlI C'TyrA_G Cuts at: 0 673 1338 1392 1617 Size: 673 665 54 225 Fragments arranged by size: 673 665 225 54 SphI G_CATG'C Cuts at: 0 135 1617 Size: 135 1482 SrfI GCCC'GGGC Cuts at: 0 40 1493 1617 Size: 40 1453 124 Fragments arranged by size: 1453 124 40 StyI C'CwwG_G Cuts at: 0 242 347 512 767 1280 1617 Size: 242 105 165 255 513 337 Fragments arranged by size: 513 337 255 242 165 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 309 1430 1617 Size: 309 1121 187 Fragments arranged by size: 1121 309 187 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 61 93 1028 1060 1561 1593 1617 Size: 61 32 935 32 501 32 24 Fragments arranged by size: 935 501 61 32 32 32 24 Tth111I GACn'n_nGTC Cuts at: 0 483 1617 Size: 483 1134 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 405 1617 Size: 405 1212 XmaI C'CCGG_G Cuts at: 0 38 215 1448 1491 1535 1617 Size: 38 177 1233 43 44 82 Fragments arranged by size: 1233 177 82 44 43 38 Enzymes that do cut: AarI Acc65I AcuI AflII AleI AlwNI ApaI AvaI BanI BanII BbeI BbvCI BclI BglI Bme1580I BmgBI BmtI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI SfoI SmaI SmlI SphI SrfI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BfrBI BglII BlpI BmrI BplI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI EarI EcoRI EcoRV FalI FspAI HincII HindIII HpaI MfeI MluI NdeI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI XbaI XhoI XmnI ZraI