################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvrII BalI BamHI BanI BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI SwaI TfiI TseI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 385 GACGTC GACGT^C AccII 69 CGCG CG^CG AlwI 281 GGATC GGATC(4/5) AlwNI 546 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 172 GAGCAC G[AT]GC[AT]^C AvaII 321 GGTCC G^G[AT]CC AviII 351 TGCGCA TGC^GCA BanII 145 GAGCCC G[GA]GC[CT]^C BpmI 329 CTGGAG CTGGAG(16/14) BsiHKAI 172 GAGCAC G[AT]GC[AT]^C BsmAI 554 GTCTC GTCTC(1/5) BsmFI 95 GGGAC GGGAC(10/14) BsmI 514 GAATGC GAATGC(1/-1) BsrI 328 ACTGG ACTGG(1/-1) BssHII 68 GCGCGC G^CGCGC BstF5I 558 CATCC GGATG(2/0) BstNI 128 CCAGG CC^[AT]GG BstUI 69 CGCG CG^CG BstYI 281 GGATCT [GA]^GATC[CT] DpnII 282 GATC ^GATC DraI 202 TTTAAA TTT^AAA EcoRII 128 CCAGG ^CC[AT]GG EcoT14I 40 CCAAGG C^C[AT][AT]GG Fnu4HI 461 GCGGC GC^[ACGT]GC FokI 558 CATCC GGATG(9/13) FspI 351 TGCGCA TGC^GCA HphI 389 TCACC GGTGA(8/7) ItaI 461 GCGGC GC^[ACGT]GC MaeIII 388 GTCAC ^GT[ACGT]AC MboI 282 GATC ^GATC MflI 281 GGATCT [GA]^GATC[CT] MvaI 128 CCAGG CC^[AT]GG MvnI 69 CGCG CG^CG NdeII 282 GATC ^GATC PstI 11 CTGCAG CTGCA^G Sau3AI 282 GATC ^GATC SbfI 10 CCTGCAGG CCTGCA^GG SfaNI 184 GATGC GCATC(5/9) Sse8387I 10 CCTGCAGG CCTGCA^GG StuI 365 AGGCCT AGG^CCT StyI 40 CCAAGG C^C[AT][AT]GG TaqI 191 TCGA T^CGA Tsp45I 388 GTCAC ^GT[GC]AC TthHB8I 191 TCGA T^CGA XhoII 281 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 30 GACGCC G[GA]^CG[CT]C 385 GACGTC AluI 379 AGCT AG^CT 547 AGCT BcnI 92 CCGGG CC^[GC]GG 497 CCGGG BsaHI 30 GACGCC G[GA]^CG[CT]C 385 GACGTC BseRI 46 GAGGAG GAGGAG(10/8) 79 GAGGAG BsgI 58 GTGCAG GTGCAG(16/14) 264 GTGCAG BsiYI 39 CCCAAGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 312 CCATCAGAGGG BslI 39 CCCAAGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 312 CCATCAGAGGG Cfr13I 116 GGCCC G^G[ACGT]CC 321 GGTCC EarI 63 GAAGAG CTCTTC(1/4) 169 GAAGAG HaeIII 116 GGCC GG^CC 366 GGCC Hin1I 30 GACGCC G[GA]^CG[CT]C 385 GACGTC HinfI 156 GAGTC G^A[ACGT]TC 405 GACTC Ksp632I 63 GAAGAG CTCTTC(1/4) 169 GAAGAG NciI 92 CCGGG CC^[GC]GG 497 CCGGG PleI 405 GACTC GAGTC(4/5) 156 GAGTC SapI 63 GAAGAGC GCTCTTC(1/4) 169 GAAGAGC Sau96I 116 GGCCC G^G[ACGT]CC 321 GGTCC SfcI 11 CTGCAG C^T[GA][CT]AG 543 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI Asp700I BbsI BmyI BpuAI BsaJI Bsp1286I BssKI Cac8I CfoI DdeI FauI HapII HgaI HhaI HinP1I HpaII MaeII MboII MnlI MseI MspI MwoI NlaIV ScrFI Tru9I TspRI XmnI