Restriction map for
pFN21AE3013
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstF5I BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I EcoT22I FbaI FokI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HpaII KasI
KpnI Ksp632I KspI MaeI MaeII
MamI MfeI MflI MluI MroI
MseI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AfaI 180 GTAC GT^AC
AvaII 113 GGACC G^G[AT]CC
BalI 233 TGGCCA TGG^CCA
BcgI 286 CGAGACCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 287 GAGACC GGTCTC(1/5)
BsaJI 200 CCGTGG C^C[ACGT][ACGT]GG
BsgI 19 GTGCAG GTGCAG(16/14)
BsiYI 5 CCTCTCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 5 CCTCTCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 287 GAGAC GTCTC(1/5)
BspMI 79 ACCTGC ACCTGC(4/8)
BsrBI 210 CCGCTC CCGCTC(-3/-3)
BsrI 98 CCAGT ACTGG(1/-1)
BssKI 41 CCTGG ^CC[ACGT]GG
BssSI 31 CTCGTG CACGAG(-5/-1)
BstAPI 21 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 204 GGTGACC G^GT[ACGT]ACC
BstNI 41 CCTGG CC^[AT]GG
BstPI 204 GGTGACC G^GT[ACGT]ACC
DpnII 270 GATC ^GATC
DraII 62 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 200 CCGTGG C^C[GA][CT]GG
EaeI 233 TGGCCA [CT]^GGCC[GA]
EcoO109I 62 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 204 GGTGACC G^GT[ACGT]ACC
EcoRII 41 CCTGG ^CC[AT]GG
MboI 270 GATC ^GATC
MboII 272 TCTTC GAAGA(8/7)
MluNI 233 TGGCCA TGG^CCA
MscI 233 TGGCCA TGG^CCA
MslI 156 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 41 CCTGG CC^[AT]GG
NdeII 270 GATC ^GATC
PstI 108 CTGCAG CTGCA^G
PvuII 22 CAGCTG CAG^CTG
RsaI 180 GTAC GT^AC
Sau3AI 270 GATC ^GATC
SbfI 107 CCTGCAGG CCTGCA^GG
ScrFI 41 CCTGG CC^[ACGT]GG
SfaNI 251 GCATC GCATC(5/9)
SfcI 108 CTGCAG C^T[GA][CT]AG
SmlI 67 CTCAAG C^T[CT][GA]AG
Sse8387I 107 CCTGCAGG CCTGCA^GG
TspRI 99 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 221 CCAGCTCCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AluI 23 AGCT AG^CT
223 AGCT
AlwNI 22 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
222 CAGCTCCTG
BanII 88 GAGCCC G[GA]GC[CT]^C
195 GAGCCC
BmyI 88 GAGCCC G[AGT]GC[ACT]^C
195 GAGCCC
BpmI 219 CTCCAG CTGGAG(16/14)
42 CTGGAG
Bsp1286I 88 GAGCCC G[AGT]GC[ACT]^C
195 GAGCCC
Cac8I 128 GCAAGC GC[ACGT]^[ACGT]GC
145 GCCTGC
FauI 122 CCCGC CCCGC(4/6)
209 CCCGC
MaeIII 163 GTGAC ^GT[ACGT]AC
205 GTGAC
MspA1I 22 CAGCTG C[AC]G^C[GT]G
123 CCGCTG
MwoI 21 GCAGCTGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
74 GCTACACCTGC
NlaIV 87 GGAGCC GG[ACGT]^[ACGT]CC
256 GGGGCC
Tsp45I 163 GTGAC ^GT[GC]AC
205 GTGAC
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
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AciI BbvI Cfr13I Fnu4HI HaeIII
HphI ItaI MnlI NlaIII Sau96I
TseI