Restriction map for
pFN21AE1656
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HinfI HpaI
KpnI Ksp632I MaeI MaeII MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MunI NaeI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 412 CGCG CG^CG
Alw44I 304 GTGCAC G^TGCAC
AlwNI 466 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 304 GTGCAC G^TGCAC
AspHI 304 GTGCAC G[AT]GC[AT]^C
AvaII 105 GGACC G^G[AT]CC
BanI 219 GGCGCC G^G[CT][GA]CC
BanII 329 GGGCTC G[GA]GC[CT]^C
BbeI 219 GGCGCC GGCGC^C
BbsI 244 GAAGAC GAAGAC(2/6)
BpuAI 244 GAAGAC GAAGAC(2/6)
BsiHKAI 304 GTGCAC G[AT]GC[AT]^C
BsrBI 363 GAGCGG CCGCTC(-3/-3)
BsrFI 455 ACCGGC [GA]^CCGG[CT]
BstUI 412 CGCG CG^CG
Cfr10I 455 ACCGGC [GA]^CCGG[CT]
DraIII 307 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 411 CCGCGG C^C[GA][CT]GG
EaeI 202 TGGCCG [CT]^GGCC[GA]
EcoNI 258 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 382 GACGC GACGC(5/10)
KasI 219 GGCGCC G^GCGCC
KspI 411 CCGCGG CCGC^GG
MaeIII 151 GTGAC ^GT[ACGT]AC
MvnI 412 CGCG CG^CG
NarI 219 GGCGCC GG^CGCC
NlaIII 48 CATG CATG^
NspI 47 ACATGC [GA]CATG^[CT]
PpuMI 104 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 291 CAGCTG CAG^CTG
SacII 411 CCGCGG CCGC^GG
SfaNI 185 GCATC GCATC(5/9)
SfoI 219 GGCGCC GGC^GCC
Tsp45I 151 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 219 GGCGCC G[GA]^CG[CT]C
382 GACGCC
AfaI 117 GTAC GT^AC
375 GTAC
BlpI 87 GCTCAGC GC^T[ACGT]AGC
252 GCTGAGC
BmyI 304 GTGCAC G[AGT]GC[ACT]^C
329 GGGCTC
Bpu1102I 87 GCTCAGC GC^T[ACGT]AGC
252 GCTGAGC
BsaHI 219 GGCGCC G[GA]^CG[CT]C
382 GACGCC
Bsp1286I 304 GTGCAC G[AGT]GC[ACT]^C
329 GGGCTC
CelII 87 GCTCAGC GC^T[ACGT]AGC
252 GCTGAGC
DdeI 88 CTCAG C^T[ACGT]AG
253 CTGAG
DraII 104 AGGACCT [GA]G^G[ACGT]CC[CT]
281 AGGCCCC
EcoO109I 104 AGGACCT [GA]G^G[ACGT]CC[CT]
281 AGGCCCC
Hin1I 219 GGCGCC G[GA]^CG[CT]C
382 GACGCC
MboII 111 GAAGA GAAGA(8/7)
244 GAAGA
MslI 129 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
189 CACCGTTGTG
MwoI 311 GCGTGGCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
401 GCCAGAAGCGC
RsaI 117 GTAC GT^AC
375 GTAC
TspRI 129 CACTG CA[GC]TG[ACGT][ACGT]^
307 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbvI BcnI BpmI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MnlI MspA1I MspI
MvaI NciI NlaIV Sau96I ScrFI
TseI