(Linear) (Six-base) MAPSORT of: KIEE1656.seq Check: 3412 from: 1 to: 519 KIEE1656.orf With 182 enzymes: SgfI * July 15, 2010 18:44 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 519 Size: 130 389 AleI CACnn'nnGTG Cuts at: 0 133 193 519 Size: 133 60 326 Fragments arranged by size: 326 133 60 AlwNI CAG_nnn'CTG Cuts at: 0 471 519 Size: 471 48 ApaLI G'TGCA_C Cuts at: 0 304 519 Size: 304 215 BanI G'GyrC_C Cuts at: 0 219 519 Size: 219 300 BanII G_rGCy'C Cuts at: 0 333 519 Size: 333 186 BbeI G_GCGC'C Cuts at: 0 223 519 Size: 223 296 BbsI GAAGACnn'nnnn_ Cuts at: 0 251 519 Size: 251 268 BlpI GC'TnA_GC Cuts at: 0 88 253 519 Size: 88 165 266 Fragments arranged by size: 266 165 88 Bme1580I G_kGCm'C Cuts at: 0 308 519 Size: 308 211 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 317 466 519 Size: 193 124 149 53 Fragments arranged by size: 193 149 124 53 BsaHI Gr'CG_yC Cuts at: 0 220 383 519 Size: 220 163 136 Fragments arranged by size: 220 163 136 BsiHKAI G_wGCw'C Cuts at: 0 308 519 Size: 308 211 Bsp1286I G_dGCh'C Cuts at: 0 308 333 519 Size: 308 25 186 Fragments arranged by size: 308 186 25 BsrBI CCG'CTC Cuts at: 0 365 519 Size: 365 154 BsrFI r'CCGG_y Cuts at: 0 455 519 Size: 455 64 BtgI C'CryG_G Cuts at: 0 411 519 Size: 411 108 BtsI GCAGTG_nn' Cuts at: 0 306 519 Size: 306 213 DraIII CAC_nnn'GTG Cuts at: 0 312 519 Size: 312 207 EaeI y'GGCC_r Cuts at: 0 202 519 Size: 202 317 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 388 519 Size: 388 131 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 193 317 466 519 Size: 130 63 124 149 53 Fragments arranged by size: 149 130 124 63 53 EcoNI CCTnn'n_nnAGG Cuts at: 0 262 519 Size: 262 257 EcoO109I rG'GnC_Cy Cuts at: 0 105 282 519 Size: 105 177 237 Fragments arranged by size: 237 177 105 HaeII r_GCGC'y Cuts at: 0 223 349 411 519 Size: 223 126 62 108 Fragments arranged by size: 223 126 108 62 KasI G'GCGC_C Cuts at: 0 219 519 Size: 219 300 MslI CAynn'nnrTG Cuts at: 0 133 193 519 Size: 133 60 326 Fragments arranged by size: 326 133 60 MspA1I CmG'CkG Cuts at: 0 171 293 413 519 Size: 171 122 120 106 Fragments arranged by size: 171 122 120 106 NarI GG'CG_CC Cuts at: 0 220 519 Size: 220 299 NspI r_CATG'y Cuts at: 0 51 519 Size: 51 468 PpuMI rG'GwC_Cy Cuts at: 0 105 519 Size: 105 414 PvuII CAG'CTG Cuts at: 0 293 519 Size: 293 226 SacII CC_GC'GG Cuts at: 0 414 519 Size: 414 105 SfoI GGC'GCC Cuts at: 0 221 519 Size: 221 298 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 79 519 Size: 79 440 Enzymes that do cut: AcuI AleI AlwNI ApaLI BanI BanII BbeI BbsI BlpI Bme1580I BpmI BsaHI BsiHKAI Bsp1286I BsrBI BsrFI BtgI BtsI DraIII EaeI EciI Eco57MI EcoNI EcoO109I HaeII KasI MslI MspA1I NarI NspI PpuMI PvuII SacII SfoI TaqII Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI