Restriction map for
pFN21AE5348
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcsI AcyI AflII AflIII
AgeI Alw44I AlwI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsiYI BslI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeIII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII HphI
KasI KpnI Ksp632I KspI MaeIII
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AclI 658 AACGTT AA^CGTT
AfaI 553 GTAC GT^AC
AhdI 291 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 452 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 271 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 291 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 409 CCCGAG C^[CT]CG[GA]G
AvaII 132 GGTCC G^G[AT]CC
BanII 746 GAGCTC G[GA]GC[CT]^C
BbsI 817 GAAGAC GAAGAC(2/6)
BciVI 84 GTATCC GTATCC(6/5)
BfaI 629 CTAG C^TAG
BglII 615 AGATCT A^GATCT
BpuAI 817 GAAGAC GAAGAC(2/6)
BsaI 207 GAGACC GGTCTC(1/5)
BseRI 115 GAGGAG GAGGAG(10/8)
BsmAI 207 GAGAC GTCTC(1/5)
BsmI 389 GAATGC GAATGC(1/-1)
BsoBI 409 CCCGAG C^[CT]CG[GA]G
BspMI 849 GCAGGT ACCTGC(4/8)
BssKI 25 CCTGG ^CC[ACGT]GG
BstAPI 451 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 25 CCTGG CC^[AT]GG
BstYI 615 AGATCT [GA]^GATC[CT]
Cfr13I 132 GGTCC G^G[ACGT]CC
DpnII 616 GATC ^GATC
Eam1105I 291 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 25 CCTGG ^CC[AT]GG
MaeI 629 CTAG C^TAG
MboI 616 GATC ^GATC
MflI 615 AGATCT [GA]^GATC[CT]
MslI 154 CATCGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 25 CCTGG CC^[AT]GG
NdeII 616 GATC ^GATC
PleI 150 GAGTC GAGTC(4/5)
Psp1406I 658 AACGTT AA^CGTT
RsaI 553 GTAC GT^AC
SacI 746 GAGCTC GAGCT^C
Sau3AI 616 GATC ^GATC
Sau96I 132 GGTCC G^G[ACGT]CC
ScrFI 25 CCTGG CC^[ACGT]GG
SmlI 202 CTTGAG C^T[CT][GA]AG
TfiI 29 GAATC G^A[AT]TC
TspRI 62 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 615 AGATCT [GA]^GATC[CT]
XmnI 271 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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Aor51HI 143 AGCGCT AGC^GCT
502 AGCGCT
BsaJI 24 CCCTGG C^C[ACGT][ACGT]GG
410 CCGAGG
BstF5I 779 CATCC GGATG(2/0)
414 GGATG
CfoI 144 GCGC GCG^C
503 GCGC
DdeI 217 CTAAG C^T[ACGT]AG
419 CTAAG
Eco47III 143 AGCGCT AGC^GCT
502 AGCGCT
FokI 779 CATCC GGATG(9/13)
414 GGATG
HaeII 143 AGCGCT [GA]GCGC^[CT]
502 AGCGCT
HhaI 144 GCGC GCG^C
503 GCGC
HinP1I 144 GCGC G^CGC
503 GCGC
HinfI 29 GAATC G^A[ACGT]TC
150 GAGTC
MaeII 599 ACGT A^CGT
659 ACGT
MspA1I 452 CAGCTG C[AC]G^C[GT]G
461 CAGCTG
NlaIII 405 CATG CATG^
649 CATG
PstI 458 CTGCAG CTGCA^G
464 CTGCAG
PvuII 452 CAGCTG CAG^CTG
461 CAGCTG
SfaNI 415 GATGC GCATC(5/9)
778 GCATC
SfcI 458 CTGCAG C^T[GA][CT]AG
464 CTGCAG
TaqI 495 TCGA T^CGA
750 TCGA
TthHB8I 495 TCGA T^CGA
750 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI AspHI BbvI BmyI BpmI
BsiHKAI Bsp1286I BsrI DraI Fnu4HI
ItaI MboII MnlI MseI MwoI
Tru9I TseI Tsp509I