(Circular) (Six-base) MAPSORT of: pFN21AE5348.seq Check: 1517 from: 1 to: 5560 pFN21AE5348 5560 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3370 278 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2903 1937 Size: 966 4594 Acc65I G'GTAC_C Cuts at: 2881 2881 Size: 5560 AclI AA'CG_TT Cuts at: 1563 2664 3687 4060 1563 Size: 1101 1023 373 3063 Fragments arranged by size: 3063 1101 1023 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3623 5007 5275 1632 Size: 1991 1384 268 1917 Fragments arranged by size: 1991 1917 1384 268 AfeI AGC'GCT Cuts at: 2150 2509 2150 Size: 359 5201 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5363 AflIII A'CryG_T Cuts at: 1203 4459 5436 1203 Size: 3256 977 1327 Fragments arranged by size: 3256 1327 977 AgeI A'CCGG_T Cuts at: 4391 4391 Size: 5560 AhdI GACnn_n'nnGTC Cuts at: 2301 4289 2301 Size: 1988 3572 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5560 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2213 1184 Size: 1029 4531 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2265 2297 2265 Size: 32 5528 AlwNI CAG_nnn'CTG Cuts at: 2462 3303 4875 2462 Size: 841 1572 3147 Fragments arranged by size: 3147 1572 841 ApaLI G'TGCA_C Cuts at: 3617 4773 3617 Size: 1156 4404 ApoI r'AATT_y Cuts at: 1493 2869 3095 3247 5356 1493 Size: 1376 226 152 2109 1697 Fragments arranged by size: 2109 1697 1376 226 152 AseI AT'TA_AT Cuts at: 160 1032 4114 160 Size: 872 3082 1606 Fragments arranged by size: 3082 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 AvaI C'yCGr_G Cuts at: 1870 1943 2414 2885 1870 Size: 73 471 471 4545 Fragments arranged by size: 4545 471 471 73 BamHI G'GATC_C Cuts at: 2890 3257 2890 Size: 367 5193 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2881 4336 618 Size: 325 217 627 1094 1455 1842 Fragments arranged by size: 1842 1455 1094 627 325 217 BanII G_rGCy'C Cuts at: 1391 2755 2879 1391 Size: 1364 124 4072 Fragments arranged by size: 4072 1364 124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2829 928 Size: 971 930 3659 Fragments arranged by size: 3659 971 930 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3751 3785 1903 Size: 34 1814 34 3678 Fragments arranged by size: 3678 1814 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2100 3452 4662 2100 Size: 1352 1210 2998 Fragments arranged by size: 2998 1352 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5560 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4171 136 Size: 107 122 71 631 605 2499 1525 Fragments arranged by size: 2499 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2620 5555 2620 Size: 2935 2625 BlpI GC'TnA_GC Cuts at: 2977 2977 Size: 5560 Bme1580I G_kGCm'C Cuts at: 1790 3621 4777 1790 Size: 1831 1156 2573 Fragments arranged by size: 2573 1831 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5560 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2191 3291 4249 Size: 416 53 83 399 24 774 1100 958 Cuts at: 4249 442 Size: 1753 Fragments arranged by size: 1753 1100 958 774 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5560 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2514 2514 Size: 5560 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2173 2292 2870 4220 1239 Size: 934 119 578 1350 2579 Fragments arranged by size: 2579 1350 934 578 119 Bpu10I CC'TnA_GC Cuts at: 2424 2424 Size: 5560 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2228 3685 4550 4848 2228 Size: 1457 865 298 2940 Fragments arranged by size: 2940 1457 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2206 4223 882 Size: 617 707 2017 2219 Fragments arranged by size: 2219 2017 707 617 BsaAI yAC'GTr Cuts at: 493 1204 3351 493 Size: 711 2147 2702 Fragments arranged by size: 2702 2147 711 BsaBI GATnn'nnATC Cuts at: 5554 5554 Size: 5560 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3367 3749 4384 275 Size: 53 83 186 936 1834 382 635 1451 Fragments arranged by size: 1834 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3993 4391 4665 4812 5223 3993 Size: 398 274 147 411 4330 Fragments arranged by size: 4330 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2146 2176 2265 2295 1178 Size: 30 165 30 743 30 89 30 4443 Fragments arranged by size: 4443 743 165 89 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 1186 Size: 949 4611 BseYI C'CCAG_C Cuts at: 4763 5259 4763 Size: 496 5064 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4987 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3010 3771 4799 1540 Size: 275 186 1009 761 1028 2301 Fragments arranged by size: 2301 1028 1009 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2056 2699 2755 3621 3706 4777 2056 Size: 643 56 866 85 1071 2839 Fragments arranged by size: 2839 1071 866 643 85 56 BsmI GAATG_Cn' Cuts at: 2400 3071 3164 2400 Size: 671 93 4796 Fragments arranged by size: 4796 671 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2699 2755 2879 3621 3706 4777 Size: 399 266 643 56 124 742 85 1071 Cuts at: 4777 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 643 399 266 124 85 56 BspEI T'CCGG_A Cuts at: 5223 5223 Size: 5560 BspHI T'CATG_A Cuts at: 3449 5179 3449 Size: 1730 3830 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2845 2915 844 Size: 2001 70 3489 Fragments arranged by size: 3489 2001 70 BsrBI CCG'CTC Cuts at: 1394 1823 3447 1394 Size: 429 1624 3507 Fragments arranged by size: 3507 1624 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4055 4229 65 Size: 1183 2807 174 1396 Fragments arranged by size: 2807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4204 4391 1700 Size: 110 2394 187 2869 Fragments arranged by size: 2869 2394 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5560 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5560 BssSI C'ACGA_G Cuts at: 3620 4632 3620 Size: 1012 4548 BstAPI GCAn_nnn'nTGC Cuts at: 794 2462 794 Size: 1668 3892 BstBI TT'CG_AA Cuts at: 4364 4364 Size: 5560 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5560 BstYI r'GATC_y Cuts at: 1972 2620 2890 3257 3642 3659 5111 5197 5209 Size: 648 270 367 385 17 1452 86 12 Cuts at: 5209 5555 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 648 385 367 346 270 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5008 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4821 Fragments arranged by size: 4821 683 56 BtsI GCAGTG_nn' Cuts at: 3076 3870 3890 3076 Size: 794 20 4746 Fragments arranged by size: 4746 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5560 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5525 DraI TTT'AAA Cuts at: 2266 2388 2554 2864 2266 Size: 122 166 310 4962 Fragments arranged by size: 4962 310 166 122 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4567 809 Size: 507 3251 1802 Fragments arranged by size: 3251 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3007 3896 5525 Size: 54 1425 50 275 18 1177 889 1629 Cuts at: 5525 8 Size: 43 Fragments arranged by size: 1629 1425 1177 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3007 1537 Size: 275 1195 4090 Fragments arranged by size: 4090 1195 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3490 1353 Size: 2137 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4147 4521 4667 387 Size: 1064 138 2558 374 146 1280 Fragments arranged by size: 2558 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2753 2753 Size: 5560 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2173 2292 2870 3623 4220 5007 5275 Size: 393 541 119 578 753 597 787 268 Cuts at: 5275 1239 Size: 1524 Fragments arranged by size: 1524 787 753 597 578 541 393 268 119 EcoRI G'AATT_C Cuts at: 2869 3247 2869 Size: 378 5182 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2695 2695 Size: 5560 FspI TGC'GCA Cuts at: 4066 4066 Size: 5560 HaeII r_GCGC'y Cuts at: 1678 2152 2511 4707 1678 Size: 474 359 2196 2531 Fragments arranged by size: 2531 2196 474 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2038 2070 2287 2319 4201 Size: 32 135 32 466 32 217 32 1882 Cuts at: 4201 4233 4275 4307 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1882 466 217 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2904 3150 669 Size: 988 281 966 246 3079 Fragments arranged by size: 3079 988 966 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5560 HpaI GTT'AAC Cuts at: 3150 3150 Size: 5560 KpnI G_GTAC'C Cuts at: 2885 2885 Size: 5560 MfeI C'AATT_G Cuts at: 3159 3159 Size: 5560 MluI A'CGCG_T Cuts at: 5436 5436 Size: 5560 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2273 4674 4858 1305 Size: 968 2401 184 2007 Fragments arranged by size: 2401 2007 968 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3738 Fragments arranged by size: 3738 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2163 3518 3877 4036 518 Size: 648 997 1355 359 159 2042 Fragments arranged by size: 2042 1355 997 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2459 2468 2976 3653 4801 5046 1606 Size: 853 9 508 677 1148 245 2120 Fragments arranged by size: 2120 1148 853 677 508 245 9 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5008 NdeI CA'TA_TG Cuts at: 387 387 Size: 5560 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5560 NotI GC'GGCC_GC Cuts at: 3007 3007 Size: 5560 NspI r_CATG'y Cuts at: 1132 1229 2918 4463 1132 Size: 97 1689 1545 2229 Fragments arranged by size: 2229 1689 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5560 PciI A'CATG_T Cuts at: 4459 4459 Size: 5560 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5560 PmeI GTTT'AAAC Cuts at: 2864 2864 Size: 5560 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2265 2297 3629 3661 5142 5174 2265 Size: 32 1332 32 1481 32 2651 Fragments arranged by size: 2651 1481 1332 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5560 PsiI TTA'TAA Cuts at: 3130 3130 Size: 5560 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2649 2681 2649 Size: 32 5528 PstI C_TGCA'G Cuts at: 830 2467 2473 2912 830 Size: 1637 6 439 3478 Fragments arranged by size: 3478 1637 439 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5560 PvuII CAG'CTG Cuts at: 2459 2468 2459 Size: 9 5551 SacI G_AGCT'C Cuts at: 2755 2755 Size: 5560 SalI G'TCGA_C Cuts at: 1936 2902 1936 Size: 966 4594 SbfI CC_TGCA'GG Cuts at: 2912 2912 Size: 5560 ScaI AGT'ACT Cuts at: 3808 5520 3808 Size: 1712 3848 SfcI C'TryA_G Cuts at: 826 1045 2463 2469 2908 4043 4724 4915 826 Size: 219 1418 6 439 1135 681 191 1471 Fragments arranged by size: 1471 1418 1135 681 439 219 191 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5560 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5560 SmaI CCC'GGG Cuts at: 1872 2887 1872 Size: 1015 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2207 3664 4565 4827 820 Size: 197 926 264 1457 901 262 1553 Fragments arranged by size: 1553 1457 926 901 264 262 197 SnaBI TAC'GTA Cuts at: 493 3351 493 Size: 2858 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5560 SphI G_CATG'C Cuts at: 1132 2918 1132 Size: 1786 3774 SspI AAT'ATT Cuts at: 5 52 3484 5 Size: 47 3432 2081 Fragments arranged by size: 3432 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5560 StyI C'CwwG_G Cuts at: 513 1065 2999 513 Size: 552 1934 3074 Fragments arranged by size: 3074 1934 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3587 3604 3757 3942 4387 325 Size: 1255 2007 17 153 185 445 1498 Fragments arranged by size: 2007 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3806 5518 96 Size: 274 80 33 51 1443 1829 1712 138 Fragments arranged by size: 1829 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5520 5552 5520 Size: 32 5528 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5560 XbaI T'CTAG_A Cuts at: 2896 2896 Size: 5560 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5560 XmaI C'CCGG_G Cuts at: 1870 2885 1870 Size: 1015 4545 XmnI GAAnn'nnTTC Cuts at: 2280 3689 2280 Size: 1409 4151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3368 276 Size: 53 83 186 936 1834 2468 Fragments arranged by size: 2468 1834 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI