(Circular) (Six-base) MAPSORT of: pFN21AE2328.seq Check: 4764 from: 1 to: 5770 pFN21AE2328 5770 bp With 182 enzymes: SgfI * February 11, 2011 18:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2139 3113 1937 Size: 202 974 4594 Fragments arranged by size: 4594 974 202 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2930 3833 5217 5485 1632 Size: 1298 903 1384 268 1917 Fragments arranged by size: 1917 1384 1298 903 268 AfeI AGC'GCT Cuts at: 2075 2075 Size: 5770 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 2586 4669 5646 1203 Size: 1383 2083 977 1327 Fragments arranged by size: 2083 1383 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 2029 4499 2029 Size: 2470 3300 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5770 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2582 1184 Size: 1398 4372 AlwNI CAG_nnn'CTG Cuts at: 2124 2632 2722 3513 5085 2124 Size: 508 90 791 1572 2809 Fragments arranged by size: 2809 1572 791 508 90 ApaLI G'TGCA_C Cuts at: 2998 3827 4983 2998 Size: 829 1156 3785 Fragments arranged by size: 3785 1156 829 ApoI r'AATT_y Cuts at: 1493 3079 3305 3457 5566 1493 Size: 1586 226 152 2109 1697 Fragments arranged by size: 2109 1697 1586 226 152 AseI AT'TA_AT Cuts at: 160 1032 2852 4324 160 Size: 872 1820 1472 1606 Fragments arranged by size: 1820 1606 1472 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2036 3095 1870 Size: 73 72 21 1059 4545 Fragments arranged by size: 4545 1059 73 72 21 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3091 4546 618 Size: 325 217 627 1304 1455 1842 Fragments arranged by size: 1842 1455 1304 627 325 217 BanII G_rGCy'C Cuts at: 1391 3089 1391 Size: 1698 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 2484 2793 928 Size: 971 161 424 309 3905 Fragments arranged by size: 3905 971 424 309 161 BbvCI CC'TCA_GC Cuts at: 2114 2114 Size: 5770 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3961 3995 1903 Size: 34 50 34 1940 34 3678 Fragments arranged by size: 3678 1940 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4381 136 Size: 107 122 71 631 605 2709 1525 Fragments arranged by size: 2709 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 2723 2780 3187 2723 Size: 57 407 5306 Fragments arranged by size: 5306 407 57 Bme1580I G_kGCm'C Cuts at: 1790 2987 3002 3831 4987 1790 Size: 1197 15 829 1156 2573 Fragments arranged by size: 2573 1197 1156 829 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2056 3501 4459 Size: 416 53 83 399 24 639 1445 958 Cuts at: 4459 442 Size: 1753 Fragments arranged by size: 1753 1445 958 639 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3040 3040 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2498 4430 1239 Size: 1259 1932 2579 Fragments arranged by size: 2579 1932 1259 Bpu10I CC'TnA_GC Cuts at: 2114 2114 Size: 5770 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3895 4760 5058 3895 Size: 865 298 4607 Fragments arranged by size: 4607 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2368 2776 3561 493 Size: 711 1164 408 785 2702 Fragments arranged by size: 2702 1164 785 711 408 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3577 3959 4594 275 Size: 53 83 186 936 2044 382 635 1451 Fragments arranged by size: 2044 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4203 4601 4875 5022 5433 4203 Size: 398 274 147 411 4540 Fragments arranged by size: 4540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2810 2840 1178 Size: 30 165 30 1407 30 4108 Fragments arranged by size: 4108 1407 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5770 BseYI C'CCAG_C Cuts at: 4973 5469 4973 Size: 496 5274 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2756 1163 Size: 573 1020 4177 Fragments arranged by size: 4177 1020 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 3220 3981 5009 1540 Size: 275 186 60 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2781 3002 3831 3916 4987 2781 Size: 221 829 85 1071 3564 Fragments arranged by size: 3564 1071 829 221 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 BsmBI CGTCTCn'nnnn_ Cuts at: 2600 2600 Size: 5770 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2781 2987 3002 3089 3831 3916 4987 Size: 399 991 206 15 87 742 85 1071 Cuts at: 4987 1391 Size: 2174 Fragments arranged by size: 2174 1071 991 742 399 206 87 85 15 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 2439 3659 5389 2439 Size: 1220 1730 2820 Fragments arranged by size: 2820 1730 1220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2694 2778 3125 844 Size: 1850 84 347 3489 Fragments arranged by size: 3489 1850 347 84 BsrBI CCG'CTC Cuts at: 1394 1823 2094 2833 3657 1394 Size: 429 271 739 824 3507 Fragments arranged by size: 3507 824 739 429 271 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2790 4414 4601 1700 Size: 110 980 1624 187 2869 Fragments arranged by size: 2869 1624 980 187 110 BsrGI T'GTAC_A Cuts at: 96 2537 96 Size: 2441 3329 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 2124 794 Size: 1330 4440 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstYI r'GATC_y Cuts at: 1972 2253 2838 3100 3467 3852 3869 5321 5407 Size: 281 585 262 367 385 17 1452 86 Cuts at: 5407 5419 5765 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 585 385 367 346 281 262 86 17 12 BstZ17I GTA'TAC Cuts at: 2140 2140 Size: 5770 BtgI C'CryG_G Cuts at: 513 1065 2287 513 Size: 552 1222 3996 Fragments arranged by size: 3996 1222 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2407 487 Size: 683 56 1181 3850 Fragments arranged by size: 3850 1181 683 56 BtsI GCAGTG_nn' Cuts at: 3000 3286 4080 4100 3000 Size: 286 794 20 4670 Fragments arranged by size: 4670 794 286 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 3217 4106 Size: 54 1425 50 275 18 228 1159 889 Cuts at: 4106 5735 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1159 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 3217 1537 Size: 275 246 1159 4090 Fragments arranged by size: 4090 1159 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2186 3700 1353 Size: 731 102 1514 3423 Fragments arranged by size: 3423 1514 731 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2498 2930 3833 4430 5217 5485 1239 Size: 393 866 432 903 597 787 268 1524 Fragments arranged by size: 1524 903 866 787 597 432 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2018 2753 2018 Size: 735 5035 EcoO109I rG'GnC_Cy Cuts at: 2737 2737 Size: 5770 EcoRI G'AATT_C Cuts at: 3079 3457 3079 Size: 378 5392 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 2077 2287 4917 1678 Size: 399 210 2630 2531 Fragments arranged by size: 2630 2531 399 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 4411 4443 4485 Size: 32 135 32 443 32 2364 32 42 Cuts at: 4485 4517 1373 Size: 32 2626 Fragments arranged by size: 2626 2364 443 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3114 3360 669 Size: 988 281 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 2310 748 Size: 1562 4208 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2361 4884 5068 1305 Size: 1056 2523 184 2007 Fragments arranged by size: 2523 2007 1056 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3948 Fragments arranged by size: 3948 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2533 2585 2591 3728 4087 4246 518 Size: 648 1367 52 6 1137 359 159 2042 Fragments arranged by size: 2042 1367 1137 648 359 159 52 6 MspA1I CmG'CkG Cuts at: 1606 2632 2875 3186 3863 5011 5256 1606 Size: 1026 243 311 677 1148 245 2120 Fragments arranged by size: 2120 1148 1026 677 311 245 243 NcoI C'CATG_G Cuts at: 513 1065 2287 513 Size: 552 1222 3996 Fragments arranged by size: 3996 1222 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 2209 2590 3128 4673 1132 Size: 97 980 381 538 1545 2229 Fragments arranged by size: 2229 1545 980 538 381 97 PasI CC'CwG_GG Cuts at: 1348 2302 1348 Size: 954 4816 PciI A'CATG_T Cuts at: 2586 4669 2586 Size: 2083 3687 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5770 PfoI T'CCnGG_A Cuts at: 2079 2975 2079 Size: 896 4874 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PmlI CAC'GTG Cuts at: 2776 2776 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2737 2737 Size: 5770 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 3122 830 Size: 2292 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 PvuII CAG'CTG Cuts at: 2632 2875 2632 Size: 243 5527 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SapI GCTCTTCn'nnn_ Cuts at: 2084 2084 Size: 5770 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 2275 4018 5730 2275 Size: 1743 1712 2315 Fragments arranged by size: 2315 1743 1712 SexAI A'CCwGG_T Cuts at: 2739 2739 Size: 5770 SfcI C'TryA_G Cuts at: 826 1045 3118 4253 4934 5125 826 Size: 219 2073 1135 681 191 1471 Fragments arranged by size: 2073 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 2038 3097 1872 Size: 166 1059 4545 Fragments arranged by size: 4545 1059 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 3874 4775 5037 820 Size: 197 926 1931 901 262 1553 Fragments arranged by size: 1931 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SspI AAT'ATT Cuts at: 5 52 2446 3694 5 Size: 47 2394 1248 2081 Fragments arranged by size: 2394 2081 1248 47 StuI AGG'CCT Cuts at: 1517 2656 2670 1517 Size: 1139 14 4617 Fragments arranged by size: 4617 1139 14 StyI C'CwwG_G Cuts at: 513 1065 2287 2918 3209 513 Size: 552 1222 631 291 3074 Fragments arranged by size: 3074 1222 631 552 291 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 2581 3797 3814 3967 4152 4597 Size: 1255 458 543 1216 17 153 185 445 Cuts at: 4597 325 Size: 1498 Fragments arranged by size: 1498 1255 1216 543 458 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2273 2537 4016 Size: 274 80 33 51 1443 296 264 1479 Cuts at: 4016 5728 96 Size: 1712 138 Fragments arranged by size: 1712 1479 1443 296 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2957 2977 2989 3009 5730 5762 2957 Size: 20 12 20 2721 32 2965 Fragments arranged by size: 2965 2721 32 20 20 12 Tth111I GACn'n_nGTC Cuts at: 1316 2741 1316 Size: 1425 4345 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 2036 3095 1870 Size: 166 1059 4545 Fragments arranged by size: 4545 1059 166 XmnI GAAnn'nnTTC Cuts at: 2075 2156 3899 2075 Size: 81 1743 3946 Fragments arranged by size: 3946 1743 81 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI