(Circular) (Six-base) MAPSORT of: pFN21AE2377.seq Check: 7298 from: 1 to: 8875 pFN21AE2377 8875 bp With 182 enzymes: SgfI * October 27, 2011 14:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6685 278 Size: 53 83 186 936 5149 2468 Fragments arranged by size: 5149 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2590 6218 1937 Size: 653 3628 4594 Fragments arranged by size: 4594 3628 653 Acc65I G'GTAC_C Cuts at: 6196 6196 Size: 8875 AclI AA'CG_TT Cuts at: 1563 7002 7375 1563 Size: 5439 373 3063 Fragments arranged by size: 5439 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2241 2568 2760 3123 3252 3957 4539 6145 Size: 609 327 192 363 129 705 582 1606 Cuts at: 6145 6938 8322 8590 1632 Size: 793 1384 268 1917 Fragments arranged by size: 1917 1606 1384 793 705 609 582 363 327 268 192 129 AflII C'TTAA_G Cuts at: 820 1017 4981 820 Size: 197 3964 4714 Fragments arranged by size: 4714 3964 197 AflIII A'CryG_T Cuts at: 1203 2216 4526 4593 7774 8751 1203 Size: 1013 2310 67 3181 977 1327 Fragments arranged by size: 3181 2310 1327 1013 977 67 AgeI A'CCGG_T Cuts at: 7706 7706 Size: 8875 AhdI GACnn_n'nnGTC Cuts at: 7604 7604 Size: 8875 AleI CACnn'nnGTG Cuts at: 1166 3075 4115 1166 Size: 1909 1040 5926 Fragments arranged by size: 5926 1909 1040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8875 AlwNI CAG_nnn'CTG Cuts at: 4105 6618 8190 4105 Size: 2513 1572 4790 Fragments arranged by size: 4790 2513 1572 ApaLI G'TGCA_C Cuts at: 6932 8088 6932 Size: 1156 7719 ApoI r'AATT_y Cuts at: 1493 2251 3175 3365 3372 3782 4187 4796 5313 Size: 758 924 190 7 410 405 609 517 Cuts at: 5313 5392 5499 6064 6184 6410 6562 8671 1493 Size: 79 107 565 120 226 152 2109 1697 Fragments arranged by size: 2109 1697 924 758 609 565 517 410 405 226 190 152 120 107 79 7 AseI AT'TA_AT Cuts at: 160 1032 4020 7429 160 Size: 872 2988 3409 1606 Fragments arranged by size: 3409 2988 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8875 AvaI C'yCGr_G Cuts at: 1870 1943 5895 6200 1870 Size: 73 3952 305 4545 Fragments arranged by size: 4545 3952 305 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5114 5147 5114 Size: 33 8842 BamHI G'GATC_C Cuts at: 6205 6572 6205 Size: 367 8508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3162 4432 5528 6196 7651 Size: 325 217 627 1375 1270 1096 668 1455 Cuts at: 7651 618 Size: 1842 Fragments arranged by size: 1842 1455 1375 1270 1096 668 627 325 217 BanII G_rGCy'C Cuts at: 1391 2066 6194 1391 Size: 675 4128 4072 Fragments arranged by size: 4128 4072 675 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2379 2619 2757 5295 5658 5769 5780 Size: 971 480 240 138 2538 363 111 11 Cuts at: 5780 6023 928 Size: 243 3780 Fragments arranged by size: 3780 2538 971 480 363 243 240 138 111 11 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7066 7100 1903 Size: 34 5129 34 3678 Fragments arranged by size: 5129 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 6767 7977 3896 Size: 2871 1210 4794 Fragments arranged by size: 4794 2871 1210 BclI T'GATC_A Cuts at: 1548 3556 1548 Size: 2008 6867 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2135 7486 136 Size: 107 122 71 631 605 463 5351 1525 Fragments arranged by size: 5351 1525 631 605 463 122 107 71 BglII A'GATC_T Cuts at: 2228 3975 8870 2228 Size: 1747 4895 2233 Fragments arranged by size: 4895 2233 1747 BlpI GC'TnA_GC Cuts at: 4830 6292 4830 Size: 1462 7413 Bme1580I G_kGCm'C Cuts at: 1790 4534 6936 8092 1790 Size: 2744 2402 1156 2573 Fragments arranged by size: 2744 2573 2402 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4877 6606 7564 Size: 416 53 83 399 24 3460 1729 958 Cuts at: 7564 442 Size: 1753 Fragments arranged by size: 3460 1753 1729 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3674 3887 1056 Size: 2618 213 6044 Fragments arranged by size: 6044 2618 213 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3803 3803 Size: 8875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 3600 4653 5232 7535 1239 Size: 878 1483 1053 579 2303 2579 Fragments arranged by size: 2579 2303 1483 1053 878 579 Bpu10I CC'TnA_GC Cuts at: 4032 4899 4032 Size: 867 8008 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5604 7000 7865 8163 5604 Size: 1396 865 298 6316 Fragments arranged by size: 6316 1396 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5639 7538 882 Size: 617 4140 1899 2219 Fragments arranged by size: 4140 2219 1899 617 BsaAI yAC'GTr Cuts at: 493 1204 2219 4594 6666 493 Size: 711 1015 2375 2072 2702 Fragments arranged by size: 2702 2375 2072 1015 711 BsaBI GATnn'nnATC Cuts at: 3246 3849 5163 5205 8869 3246 Size: 603 1314 42 3664 3252 Fragments arranged by size: 3664 3252 1314 603 42 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6682 7064 7699 275 Size: 53 83 186 936 5149 382 635 1451 Fragments arranged by size: 5149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2211 4774 5648 7308 7706 7980 8127 8538 2211 Size: 2563 874 1660 398 274 147 411 2548 Fragments arranged by size: 2563 2548 1660 874 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 1178 Size: 30 165 30 624 30 7996 Fragments arranged by size: 7996 624 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3088 5338 1186 Size: 1902 2250 4723 Fragments arranged by size: 4723 2250 1902 BseYI C'CCAG_C Cuts at: 2977 8078 8574 2977 Size: 5101 496 3278 Fragments arranged by size: 5101 3278 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2018 3475 1163 Size: 573 282 1457 6563 Fragments arranged by size: 6563 1457 573 282 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2110 2130 6325 7086 8114 1540 Size: 275 186 109 20 4195 761 1028 2301 Fragments arranged by size: 4195 2301 1028 761 275 186 109 20 BsiHKAI G_wGCw'C Cuts at: 2103 2367 3442 6936 7021 8092 2103 Size: 264 1075 3494 85 1071 2886 Fragments arranged by size: 3494 2886 1075 1071 264 85 BsmI GAATG_Cn' Cuts at: 5584 6386 6479 5584 Size: 802 93 7980 Fragments arranged by size: 7980 802 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2103 2367 3442 4534 6194 6936 Size: 399 276 37 264 1075 1092 1660 742 Cuts at: 6936 7021 8092 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1660 1092 1075 1071 742 399 276 264 85 37 BspEI T'CCGG_A Cuts at: 8538 8538 Size: 8875 BspHI T'CATG_A Cuts at: 3486 5992 6764 8494 3486 Size: 2506 772 1730 3867 Fragments arranged by size: 3867 2506 1730 772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6230 844 Size: 5386 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2124 6762 1394 Size: 429 301 4638 3507 Fragments arranged by size: 4638 3507 429 301 BsrDI GCAATG_nn' Cuts at: 65 1248 7370 7544 65 Size: 1183 6122 174 1396 Fragments arranged by size: 6122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 7519 7706 1700 Size: 110 319 5390 187 2869 Fragments arranged by size: 5390 2869 319 187 110 BsrGI T'GTAC_A Cuts at: 96 2658 5786 96 Size: 2562 3128 3185 Fragments arranged by size: 3185 3128 2562 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8875 BssSI C'ACGA_G Cuts at: 6935 7947 6935 Size: 1012 7863 BstAPI GCAn_nnn'nTGC Cuts at: 794 3236 794 Size: 2442 6433 BstBI TT'CG_AA Cuts at: 7679 7679 Size: 8875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8875 BstXI CCAn_nnnn'nTGG Cuts at: 3862 3862 Size: 8875 BstYI r'GATC_y Cuts at: 1972 2228 3745 3975 4006 5206 6205 6572 6957 Size: 256 1517 230 31 1200 999 367 385 Cuts at: 6957 6974 8426 8512 8524 8870 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1517 1452 1200 999 385 367 346 256 230 86 31 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 2058 2151 513 Size: 552 939 42 12 93 7237 Fragments arranged by size: 7237 939 552 93 42 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2190 6086 487 Size: 683 56 964 3896 3276 Fragments arranged by size: 3896 3276 964 683 56 BtsI GCAGTG_nn' Cuts at: 2541 2731 6391 7185 7205 2541 Size: 190 3660 794 20 4211 Fragments arranged by size: 4211 3660 794 190 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5386 5421 529 Size: 35 4822 35 3983 Fragments arranged by size: 4822 3983 35 35 DraI TTT'AAA Cuts at: 3370 6179 3370 Size: 2809 6066 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3027 7882 809 Size: 507 1711 4855 1802 Fragments arranged by size: 4855 1802 1711 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2107 2127 Size: 54 1425 50 275 18 225 52 20 Cuts at: 2127 2331 6322 7211 8840 8 Size: 204 3991 889 1629 43 Fragments arranged by size: 3991 1629 1425 889 275 225 204 54 52 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2107 2127 6322 1537 Size: 275 295 20 4195 4090 Fragments arranged by size: 4195 4090 295 275 20 EarI CTCTTCn'nnn_ Cuts at: 1353 2355 2736 2862 3873 4018 4713 5486 6805 Size: 1002 381 126 1011 145 695 773 1319 Cuts at: 6805 1353 Size: 3423 Fragments arranged by size: 3423 1319 1011 1002 773 695 381 145 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3142 7462 7836 7982 387 Size: 1064 138 1553 4320 374 146 1280 Fragments arranged by size: 4320 1553 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2241 2568 2760 3123 3252 3600 Size: 393 485 124 327 192 363 129 348 Cuts at: 3600 3957 4539 4653 5232 6145 6938 7535 8322 Size: 357 582 114 579 913 793 597 787 Cuts at: 8322 8590 1239 Size: 268 1524 Fragments arranged by size: 1524 913 793 787 597 582 579 485 393 363 357 348 327 268 192 129 124 114 EcoRI G'AATT_C Cuts at: 2251 3782 4187 6184 6562 2251 Size: 1531 405 1997 378 4564 Fragments arranged by size: 4564 1997 1531 405 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2573 3048 5297 2573 Size: 475 2249 6151 Fragments arranged by size: 6151 2249 475 FspI TGC'GCA Cuts at: 7381 7381 Size: 8875 HaeII r_GCGC'y Cuts at: 1678 8022 1678 Size: 6344 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2168 2200 2473 2505 3908 Size: 32 135 32 596 32 273 32 1403 Cuts at: 3908 3940 4256 4288 4892 4924 5084 5116 5402 Size: 32 316 32 604 32 160 32 286 Cuts at: 5402 5434 6107 6139 7516 7548 7590 7622 1373 Size: 32 673 32 1377 32 42 32 2626 Fragments arranged by size: 2626 1403 1377 673 604 596 316 286 273 160 135 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4144 5044 6219 6465 669 Size: 988 281 2206 900 1175 246 3079 Fragments arranged by size: 3079 2206 1175 988 900 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8875 HpaI GTT'AAC Cuts at: 6465 6465 Size: 8875 KpnI G_GTAC'C Cuts at: 6200 6200 Size: 8875 MfeI C'AATT_G Cuts at: 6474 6474 Size: 8875 MluI A'CGCG_T Cuts at: 8751 8751 Size: 8875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3880 3984 4591 6039 7989 8173 1305 Size: 2575 104 607 1448 1950 184 2007 Fragments arranged by size: 2575 2007 1950 1448 607 184 104 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 10 Size: 54 1425 343 501 6552 Fragments arranged by size: 6552 1425 501 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2439 2766 3075 4115 6833 7192 7351 Size: 648 1273 327 309 1040 2718 359 159 Cuts at: 7351 518 Size: 2042 Fragments arranged by size: 2718 2042 1273 1040 648 359 327 309 159 MspA1I CmG'CkG Cuts at: 1606 2048 2153 4439 6291 6968 8116 8361 1606 Size: 442 105 2286 1852 677 1148 245 2120 Fragments arranged by size: 2286 2120 1852 1148 677 442 245 105 NaeI GCC'GGC Cuts at: 2131 2131 Size: 8875 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7384 Fragments arranged by size: 7384 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8875 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 8875 NheI G'CTAG_C Cuts at: 1052 3670 3883 1052 Size: 2618 213 6044 Fragments arranged by size: 6044 2618 213 NotI GC'GGCC_GC Cuts at: 6322 6322 Size: 8875 NspI r_CATG'y Cuts at: 1132 1229 3065 4184 4530 5787 6233 7778 1132 Size: 97 1836 1119 346 1257 446 1545 2229 Fragments arranged by size: 2229 1836 1545 1257 1119 446 346 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8875 PciI A'CATG_T Cuts at: 4526 7774 4526 Size: 3248 5627 PflMI CCAn_nnn'nTGG Cuts at: 1108 3846 4412 1108 Size: 2738 566 5571 Fragments arranged by size: 5571 2738 566 PfoI T'CCnGG_A Cuts at: 5209 5209 Size: 8875 PmeI GTTT'AAAC Cuts at: 6179 6179 Size: 8875 PmlI CAC'GTG Cuts at: 2219 4594 2219 Size: 2375 6500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6944 6976 8457 8489 6944 Size: 32 1481 32 7330 Fragments arranged by size: 7330 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8875 PsiI TTA'TAA Cuts at: 2534 3207 6445 2534 Size: 673 3238 4964 Fragments arranged by size: 4964 3238 673 PspXI vC'TCGA_Gb Cuts at: 5895 5895 Size: 8875 PstI C_TGCA'G Cuts at: 830 4002 4167 6227 830 Size: 3172 165 2060 3478 Fragments arranged by size: 3478 3172 2060 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8875 PvuII CAG'CTG Cuts at: 4439 4439 Size: 8875 SacII CC_GC'GG Cuts at: 2049 2154 2049 Size: 105 8770 SalI G'TCGA_C Cuts at: 1936 6217 1936 Size: 4281 4594 SapI GCTCTTCn'nnn_ Cuts at: 2355 3873 2355 Size: 1518 7357 SbfI CC_TGCA'GG Cuts at: 6227 6227 Size: 8875 ScaI AGT'ACT Cuts at: 4507 5458 7123 8835 4507 Size: 951 1665 1712 4547 Fragments arranged by size: 4547 1712 1665 951 SfcI C'TryA_G Cuts at: 826 1045 3741 3998 4163 5173 5778 6223 7358 Size: 219 2696 257 165 1010 605 445 1135 Cuts at: 7358 8039 8230 826 Size: 681 191 1471 Fragments arranged by size: 2696 1471 1135 1010 681 605 445 257 219 191 165 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8875 SmaI CCC'GGG Cuts at: 1872 6202 1872 Size: 4330 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4981 5619 5895 6979 7880 8142 Size: 197 926 3038 638 276 1084 901 262 Cuts at: 8142 820 Size: 1553 Fragments arranged by size: 3038 1553 1084 926 901 638 276 262 197 SnaBI TAC'GTA Cuts at: 493 6666 493 Size: 6173 2702 SpeI A'CTAG_T Cuts at: 152 3986 152 Size: 3834 5041 SphI G_CATG'C Cuts at: 1132 3065 6233 1132 Size: 1933 3168 3774 Fragments arranged by size: 3774 3168 1933 SspI AAT'ATT Cuts at: 5 52 4389 4654 6799 5 Size: 47 4337 265 2145 2081 Fragments arranged by size: 4337 2145 2081 265 47 StuI AGG'CCT Cuts at: 1517 3563 3763 1517 Size: 2046 200 6629 Fragments arranged by size: 6629 2046 200 StyI C'CwwG_G Cuts at: 513 1065 2004 2067 6314 513 Size: 552 939 63 4247 3074 Fragments arranged by size: 4247 3074 939 552 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2385 4630 6902 6919 7072 7257 7702 Size: 1255 805 2245 2272 17 153 185 445 Cuts at: 7702 325 Size: 1498 Fragments arranged by size: 2272 2245 1498 1255 805 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2426 2554 2658 Size: 274 80 33 51 1443 449 128 104 Cuts at: 2658 4505 5456 5786 7121 8833 96 Size: 1847 951 330 1335 1712 138 Fragments arranged by size: 1847 1712 1443 1335 951 449 330 274 138 128 104 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2027 2059 8835 8867 2027 Size: 32 6776 32 2035 Fragments arranged by size: 6776 2035 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8875 XbaI T'CTAG_A Cuts at: 5324 6211 5324 Size: 887 7988 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2065 2065 Size: 8875 XhoI C'TCGA_G Cuts at: 1943 5895 1943 Size: 3952 4923 XmaI C'CCGG_G Cuts at: 1870 6200 1870 Size: 4330 4545 XmnI GAAnn'nnTTC Cuts at: 5313 5728 7004 5313 Size: 415 1276 7184 Fragments arranged by size: 7184 1276 415 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6683 276 Size: 53 83 186 936 5149 2468 Fragments arranged by size: 5149 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI