(Linear) (Six-base) MAPSORT of: KIEE2377.seq Check: 4973 from: 1 to: 4170 KIEE2377.orf With 182 enzymes: SgfI * October 27, 2011 13:18 .. AccI GT'mk_AC Cuts at: 0 585 4170 Size: 585 3585 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 236 563 755 1118 1247 1952 2534 4140 Size: 236 327 192 363 129 705 582 1606 Cuts at: 4140 4170 Size: 30 Fragments arranged by size: 1606 705 582 363 327 236 192 129 30 AflII C'TTAA_G Cuts at: 0 2976 4170 Size: 2976 1194 AflIII A'CryG_T Cuts at: 0 211 2521 2588 4170 Size: 211 2310 67 1582 Fragments arranged by size: 2310 1582 211 67 AleI CACnn'nnGTG Cuts at: 0 1070 2110 4170 Size: 1070 1040 2060 Fragments arranged by size: 2060 1070 1040 AlwNI CAG_nnn'CTG Cuts at: 0 2100 4170 Size: 2100 2070 ApoI r'AATT_y Cuts at: 0 246 1170 1360 1367 1777 2182 2791 3308 Size: 246 924 190 7 410 405 609 517 Cuts at: 3308 3387 3494 4059 4170 Size: 79 107 565 111 Fragments arranged by size: 924 609 565 517 410 405 246 190 111 107 79 7 AseI AT'TA_AT Cuts at: 0 2015 4170 Size: 2015 2155 AvaI C'yCGr_G Cuts at: 0 3890 4170 Size: 3890 280 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3109 3142 4170 Size: 3109 33 1028 Fragments arranged by size: 3109 1028 33 BanI G'GyrC_C Cuts at: 0 1157 2427 3523 4170 Size: 1157 1270 1096 647 Fragments arranged by size: 1270 1157 1096 647 BanII G_rGCy'C Cuts at: 0 61 4170 Size: 61 4109 BbsI GAAGACnn'nnnn_ Cuts at: 0 374 614 752 3290 3653 3764 3775 4018 Size: 374 240 138 2538 363 111 11 243 Cuts at: 4018 4170 Size: 152 Fragments arranged by size: 2538 374 363 243 240 152 138 111 11 BciVI GTATCCnnnnn_n' Cuts at: 0 1891 4170 Size: 1891 2279 BclI T'GATC_A Cuts at: 0 1551 4170 Size: 1551 2619 BglI GCCn_nnn'nGGC Cuts at: 0 130 4170 Size: 130 4040 BglII A'GATC_T Cuts at: 0 223 1970 4170 Size: 223 1747 2200 Fragments arranged by size: 2200 1747 223 BlpI GC'TnA_GC Cuts at: 0 2825 4170 Size: 2825 1345 Bme1580I G_kGCm'C Cuts at: 0 2529 4170 Size: 2529 1641 BmrI ACTGGGnnnn_n' Cuts at: 0 2872 4170 Size: 2872 1298 BmtI G_CTAG'C Cuts at: 0 1669 1882 4170 Size: 1669 213 2288 Fragments arranged by size: 2288 1669 213 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1798 4170 Size: 1798 2372 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 1595 2648 3227 4170 Size: 112 1483 1053 579 943 Fragments arranged by size: 1483 1053 943 579 112 Bpu10I CC'TnA_GC Cuts at: 0 2027 2894 4170 Size: 2027 867 1276 Fragments arranged by size: 2027 1276 867 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 3599 4170 Size: 3599 571 BsaI GGTCTCn'nnnn_ Cuts at: 0 3634 4170 Size: 3634 536 BsaAI yAC'GTr Cuts at: 0 214 2589 4170 Size: 214 2375 1581 Fragments arranged by size: 2375 1581 214 BsaBI GATnn'nnATC Cuts at: 0 1241 1844 3158 3200 4170 Size: 1241 603 1314 42 970 Fragments arranged by size: 1314 1241 970 603 42 BsaWI w'CCGG_w Cuts at: 0 206 2769 3643 4170 Size: 206 2563 874 527 Fragments arranged by size: 2563 874 527 206 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 22 52 4170 Size: 22 30 4118 Fragments arranged by size: 4118 30 22 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1083 3333 4170 Size: 1083 2250 837 Fragments arranged by size: 2250 1083 837 BseYI C'CCAG_C Cuts at: 0 972 4170 Size: 972 3198 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 13 1470 4170 Size: 13 1457 2700 Fragments arranged by size: 2700 1457 13 BsiEI CG_ry'CG Cuts at: 0 105 125 4170 Size: 105 20 4045 Fragments arranged by size: 4045 105 20 BsiHKAI G_wGCw'C Cuts at: 0 98 362 1437 4170 Size: 98 264 1075 2733 Fragments arranged by size: 2733 1075 264 98 BsmI GAATG_Cn' Cuts at: 0 3579 4170 Size: 3579 591 Bsp1286I G_dGCh'C Cuts at: 0 61 98 362 1437 2529 4170 Size: 61 37 264 1075 1092 1641 Fragments arranged by size: 1641 1092 1075 264 61 37 BspHI T'CATG_A Cuts at: 0 1481 3987 4170 Size: 1481 2506 183 Fragments arranged by size: 2506 1481 183 BsrBI CCG'CTC Cuts at: 0 119 4170 Size: 119 4051 BsrFI r'CCGG_y Cuts at: 0 124 4170 Size: 124 4046 BsrGI T'GTAC_A Cuts at: 0 653 3781 4170 Size: 653 3128 389 Fragments arranged by size: 3128 653 389 BstAPI GCAn_nnn'nTGC Cuts at: 0 1231 4170 Size: 1231 2939 BstXI CCAn_nnnn'nTGG Cuts at: 0 1857 4170 Size: 1857 2313 BstYI r'GATC_y Cuts at: 0 223 1740 1970 2001 3201 4170 Size: 223 1517 230 31 1200 969 Fragments arranged by size: 1517 1200 969 230 223 31 BtgI C'CryG_G Cuts at: 0 41 53 146 4170 Size: 41 12 93 4024 Fragments arranged by size: 4024 93 41 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 185 4081 4170 Size: 185 3896 89 Fragments arranged by size: 3896 185 89 BtsI GCAGTG_nn' Cuts at: 0 536 726 4170 Size: 536 190 3444 Fragments arranged by size: 3444 536 190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3381 3416 4170 Size: 3381 35 754 Fragments arranged by size: 3381 754 35 DraI TTT'AAA Cuts at: 0 1365 4170 Size: 1365 2805 DrdI GACnn_nn'nnGTC Cuts at: 0 1022 4170 Size: 1022 3148 EaeI y'GGCC_r Cuts at: 0 50 102 122 326 4170 Size: 50 52 20 204 3844 Fragments arranged by size: 3844 204 52 50 20 EagI C'GGCC_G Cuts at: 0 102 122 4170 Size: 102 20 4048 Fragments arranged by size: 4048 102 20 EarI CTCTTCn'nnn_ Cuts at: 0 350 731 857 1868 2013 2708 3481 4170 Size: 350 381 126 1011 145 695 773 689 Fragments arranged by size: 1011 773 695 689 381 350 145 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1137 4170 Size: 1137 3033 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 112 236 563 755 1118 1247 1595 1952 Size: 112 124 327 192 363 129 348 357 Cuts at: 1952 2534 2648 3227 4140 4170 Size: 582 114 579 913 30 Fragments arranged by size: 913 582 579 363 357 348 327 192 129 124 114 112 30 EcoRI G'AATT_C Cuts at: 0 246 1777 2182 4170 Size: 246 1531 405 1988 Fragments arranged by size: 1988 1531 405 246 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 568 1043 3292 4170 Size: 568 475 2249 878 Fragments arranged by size: 2249 878 568 475 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 163 195 468 500 1903 1935 2251 2283 Size: 163 32 273 32 1403 32 316 32 Cuts at: 2283 2887 2919 3079 3111 3397 3429 4102 4134 Size: 604 32 160 32 286 32 673 32 Cuts at: 4134 4170 Size: 36 Fragments arranged by size: 1403 673 604 316 286 273 163 160 36 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 2139 3039 4170 Size: 2139 900 1131 Fragments arranged by size: 2139 1131 900 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1875 1979 2586 4034 4170 Size: 1875 104 607 1448 136 Fragments arranged by size: 1875 1448 607 136 104 MscI TGG'CCA Cuts at: 0 328 4170 Size: 328 3842 MslI CAynn'nnrTG Cuts at: 0 434 761 1070 2110 4170 Size: 434 327 309 1040 2060 Fragments arranged by size: 2060 1040 434 327 309 MspA1I CmG'CkG Cuts at: 0 43 148 2434 4170 Size: 43 105 2286 1736 Fragments arranged by size: 2286 1736 105 43 NaeI GCC'GGC Cuts at: 0 126 4170 Size: 126 4044 NgoMIV G'CCGG_C Cuts at: 0 124 4170 Size: 124 4046 NheI G'CTAG_C Cuts at: 0 1665 1878 4170 Size: 1665 213 2292 Fragments arranged by size: 2292 1665 213 NspI r_CATG'y Cuts at: 0 1060 2179 2525 3782 4170 Size: 1060 1119 346 1257 388 Fragments arranged by size: 1257 1119 1060 388 346 PciI A'CATG_T Cuts at: 0 2521 4170 Size: 2521 1649 PflMI CCAn_nnn'nTGG Cuts at: 0 1841 2407 4170 Size: 1841 566 1763 Fragments arranged by size: 1841 1763 566 PfoI T'CCnGG_A Cuts at: 0 3204 4170 Size: 3204 966 PmlI CAC'GTG Cuts at: 0 214 2589 4170 Size: 214 2375 1581 Fragments arranged by size: 2375 1581 214 PsiI TTA'TAA Cuts at: 0 529 1202 4170 Size: 529 673 2968 Fragments arranged by size: 2968 673 529 PspXI vC'TCGA_Gb Cuts at: 0 3890 4170 Size: 3890 280 PstI C_TGCA'G Cuts at: 0 1997 2162 4170 Size: 1997 165 2008 Fragments arranged by size: 2008 1997 165 PvuII CAG'CTG Cuts at: 0 2434 4170 Size: 2434 1736 SacII CC_GC'GG Cuts at: 0 44 149 4170 Size: 44 105 4021 Fragments arranged by size: 4021 105 44 SapI GCTCTTCn'nnn_ Cuts at: 0 350 1868 4170 Size: 350 1518 2302 Fragments arranged by size: 2302 1518 350 ScaI AGT'ACT Cuts at: 0 2502 3453 4170 Size: 2502 951 717 Fragments arranged by size: 2502 951 717 SfcI C'TryA_G Cuts at: 0 1736 1993 2158 3168 3773 4170 Size: 1736 257 165 1010 605 397 Fragments arranged by size: 1736 1010 605 397 257 165 SmlI C'TyrA_G Cuts at: 0 2976 3614 3890 4170 Size: 2976 638 276 280 Fragments arranged by size: 2976 638 280 276 SpeI A'CTAG_T Cuts at: 0 1981 4170 Size: 1981 2189 SphI G_CATG'C Cuts at: 0 1060 4170 Size: 1060 3110 SspI AAT'ATT Cuts at: 0 2384 2649 4170 Size: 2384 265 1521 Fragments arranged by size: 2384 1521 265 StuI AGG'CCT Cuts at: 0 1558 1758 4170 Size: 1558 200 2412 Fragments arranged by size: 2412 1558 200 StyI C'CwwG_G Cuts at: 0 62 4170 Size: 62 4108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 380 2625 4170 Size: 380 2245 1545 Fragments arranged by size: 2245 1545 380 TatI w'GTAC_w Cuts at: 0 421 549 653 2500 3451 3781 4170 Size: 421 128 104 1847 951 330 389 Fragments arranged by size: 1847 951 421 389 330 128 104 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 22 54 4170 Size: 22 32 4116 Fragments arranged by size: 4116 32 22 XbaI T'CTAG_A Cuts at: 0 3319 4170 Size: 3319 851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 60 4170 Size: 60 4110 XhoI C'TCGA_G Cuts at: 0 3890 4170 Size: 3890 280 XmnI GAAnn'nnTTC Cuts at: 0 3308 3723 4170 Size: 3308 415 447 Fragments arranged by size: 3308 447 415 Enzymes that do cut: AccI AcuI AflII AflIII AleI AlwNI ApoI AseI AvaI BaeI BanI BanII BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI Hin4I HincII MmeI MscI MslI MspA1I NaeI NgoMIV NheI NspI PciI PflMI PfoI PmlI PsiI PspXI PstI PvuII SacII SapI ScaI SfcI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BamHI BbeI BbvCI BcgI BfrBI BmgBI BsaHI BsiWI BsmBI BspEI BspMI BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NotI NruI NsiI PacI PasI PmeI PpiI PpuMI PshAI PspOMI PsrI PvuI RsrII SacI SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI Tth111I XmaI ZraI