################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BclI BglII BlnI BpmI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KpnI KspI MluI MluNI MroI MscI MslI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AclI 1545 AACGTT AA^CGTT AflII 294 CTTAAG C^TTAAG Asp700I 447 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 148 GGTGCC G^G[CT][GA]CC BbsI 1300 GTCTTC GAAGAC(2/6) BbvCI 762 CCTCAGC CCTCAGC(-5/-2) BciVI 23 GTATCC GTATCC(6/5) BfrI 294 CTTAAG C^TTAAG BglI 151 GCCTTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 963 GCTGAGC GC^T[ACGT]AGC Bpu1102I 963 GCTGAGC GC^T[ACGT]AGC BpuAI 1300 GTCTTC GAAGAC(2/6) BsaAI 508 CACGTA [CT]AC^GT[GA] BsaBI 390 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1249 GGTCTC GGTCTC(1/5) BspLU11I 1328 ACATGT A^CATGT CelII 963 GCTGAGC GC^T[ACGT]AGC DraI 732 TTTAAA TTT^AAA DrdI 477 GACAGATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 698 CCATGG C^C[GA][CT]GG FauI 1345 CCCGC CCCGC(4/6) HgaI 1581 GACGC GACGC(5/10) HincII 1230 GTTAAC GT[CT]^[GA]AC HindII 1230 GTTAAC GT[CT]^[GA]AC HpaI 1230 GTTAAC GTT^AAC MamI 390 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 698 CCATGG C^CATGG PpuMI 465 GGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 1545 AACGTT AA^CGTT SapI 1188 GCTCTTC GCTCTTC(1/4) SfcI 1022 CTATAG C^T[GA][CT]AG SmlI 294 CTTAAG C^T[CT][GA]AG SphI 1558 GCATGC GCATG^C Tsp45I 486 GTCAC ^GT[GC]AC XmnI 447 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AciI 1346 CCGC CCGC(-3/-1) 1617 GCGG AcsI 907 GAATTT [GA]^AATT[CT] 1205 AAATTT AflIII 507 ACACGT A^C[GA][CT]GT 1328 ACATGT ApoI 907 GAATTT [GA]^AATT[CT] 1205 AAATTT AspHI 199 GAGCAC G[AT]GC[AT]^C 596 GAGCAC AvaII 466 GGTCC G^G[AT]CC 844 GGACC BcgI 953 CGAACCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 997 CGATCTAAGTGC BfaI 223 CTAG C^TAG 1243 CTAG BseRI 64 CTCCTC GAGGAG(10/8) 1219 CTCCTC BsiHKAI 199 GAGCAC G[AT]GC[AT]^C 596 GAGCAC BsrDI 1170 CATTGC GCAATG(2/0) 1096 GCAATG DraII 465 GGGTCCC [GA]G^G[ACGT]CC[CT] 802 AGGGCCT EarI 1189 CTCTTC CTCTTC(1/4) 1222 CTCTTC EcoO109I 465 GGGTCCC [GA]G^G[ACGT]CC[CT] 802 AGGGCCT EcoT14I 69 CCTTGG C^C[AT][AT]GG 698 CCATGG EcoT22I 1 ATGCAT ATGCA^T 1556 ATGCAT HaeIII 804 GGCC GG^CC 1505 GGCC Ksp632I 1189 CTCTTC CTCTTC(1/4) 1222 CTCTTC MaeI 223 CTAG C^TAG 1243 CTAG MaeII 509 ACGT A^CGT 1546 ACGT MaeIII 486 GTCAC ^GT[ACGT]AC 511 GTAAC MfeI 517 CAATTG C^AATTG 1361 CAATTG MspA1I 80 CAGCTG C[AC]G^C[GT]G 1272 CAGCTG MunI 517 CAATTG C^AATTG 1361 CAATTG NsiI 1 ATGCAT ATGCA^T 1556 ATGCAT NspI 1328 ACATGT [GA]CATG^[CT] 1558 GCATGC PvuII 80 CAGCTG CAG^CTG 1272 CAGCTG StyI 69 CCTTGG C^C[AT][AT]GG 698 CCATGG TaqI 996 TCGA T^CGA 1333 TCGA TthHB8I 996 TCGA T^CGA 1333 TCGA XcmI 921 CCAATGTAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1438 CCAGTATCAGTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AfaI AluI AlwI BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HapII HinfI HpaII HphI ItaI MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI TfiI Tru9I TseI Tsp509I TspRI XhoII