(Circular) (Six-base) MAPSORT of: pFN21AE5683.seq Check: 606 from: 1 to: 6361 pFN21AE5683 6361 bp With 182 enzymes: SgfI * June 11, 2014 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4171 278 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3704 1937 Size: 1767 4594 Acc65I G'GTAC_C Cuts at: 3682 3682 Size: 6361 AclI AA'CG_TT Cuts at: 1563 3551 4488 4861 1563 Size: 1988 937 373 3063 Fragments arranged by size: 3063 1988 937 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2389 2581 4424 5808 6076 1632 Size: 757 192 1843 1384 268 1917 Fragments arranged by size: 1917 1843 1384 757 268 192 AflII C'TTAA_G Cuts at: 820 1017 2299 820 Size: 197 1282 4882 Fragments arranged by size: 4882 1282 197 AflIII A'CryG_T Cuts at: 1203 2512 3333 5260 6237 1203 Size: 1309 821 1927 977 1327 Fragments arranged by size: 1927 1327 1309 977 821 AgeI A'CCGG_T Cuts at: 5192 5192 Size: 6361 AhdI GACnn_n'nnGTC Cuts at: 5090 5090 Size: 6361 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6361 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6361 AlwNI CAG_nnn'CTG Cuts at: 4104 5676 4104 Size: 1572 4789 ApaLI G'TGCA_C Cuts at: 4418 5574 4418 Size: 1156 5205 ApoI r'AATT_y Cuts at: 1493 2912 3210 3670 3896 4048 6157 1493 Size: 1419 298 460 226 152 2109 1697 Fragments arranged by size: 2109 1697 1419 460 298 226 152 AseI AT'TA_AT Cuts at: 160 1032 4915 160 Size: 872 3883 1606 Fragments arranged by size: 3883 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 AvaI C'yCGr_G Cuts at: 1870 1943 3686 1870 Size: 73 1743 4545 Fragments arranged by size: 4545 1743 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2527 2560 2527 Size: 33 6328 BamHI G'GATC_C Cuts at: 3691 4058 3691 Size: 367 5994 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 3682 5137 618 Size: 325 217 627 366 1529 1455 1842 Fragments arranged by size: 1842 1529 1455 627 366 325 217 BanII G_rGCy'C Cuts at: 1391 3680 1391 Size: 2289 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3298 928 Size: 971 1399 3991 Fragments arranged by size: 3991 1399 971 BbvCI CC'TCA_GC Cuts at: 2768 2768 Size: 6361 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2947 2981 2991 3025 4552 Size: 34 50 34 926 34 10 34 1527 Cuts at: 4552 4586 1903 Size: 34 3678 Fragments arranged by size: 3678 1527 926 50 34 34 34 34 34 10 BciVI GTATCCnnnnn_n' Cuts at: 2039 4253 5463 2039 Size: 2214 1210 2937 Fragments arranged by size: 2937 2214 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6361 BfrBI ATG'CAT Cuts at: 2008 3563 2008 Size: 1555 4806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2162 4972 136 Size: 107 122 71 631 605 490 2810 1525 Fragments arranged by size: 2810 1525 631 605 490 122 107 71 BglII A'GATC_T Cuts at: 6356 6356 Size: 6361 BlpI GC'TnA_GC Cuts at: 2969 3778 2969 Size: 809 5552 Bme1580I G_kGCm'C Cuts at: 1790 3398 4422 5578 1790 Size: 1608 1024 1156 2573 Fragments arranged by size: 2573 1608 1156 1024 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6361 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2615 4092 5050 Size: 416 53 83 399 24 1198 1477 958 Cuts at: 5050 442 Size: 1753 Fragments arranged by size: 1753 1477 1198 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6361 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2084 2084 Size: 6361 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5021 1239 Size: 3782 2579 Bpu10I CC'TnA_GC Cuts at: 2768 2768 Size: 6361 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4486 5351 5649 4486 Size: 865 298 5198 Fragments arranged by size: 5198 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3260 5024 882 Size: 617 1761 1764 2219 Fragments arranged by size: 2219 1764 1761 617 BsaAI yAC'GTr Cuts at: 493 1204 2515 4152 493 Size: 711 1311 1637 2702 Fragments arranged by size: 2702 1637 1311 711 BsaBI GATnn'nnATC Cuts at: 2399 6355 2399 Size: 3956 2405 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4168 4550 5185 275 Size: 53 83 186 936 2635 382 635 1451 Fragments arranged by size: 2635 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4794 5192 5466 5613 6024 4794 Size: 398 274 147 411 5131 Fragments arranged by size: 5131 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2855 2885 1178 Size: 30 165 30 1452 30 4654 Fragments arranged by size: 4654 1452 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 3215 1186 Size: 874 1155 4332 Fragments arranged by size: 4332 1155 874 BseYI C'CCAG_C Cuts at: 2659 3386 5564 6060 2659 Size: 727 2178 496 2960 Fragments arranged by size: 2960 2178 727 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5788 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3811 4572 5600 1540 Size: 275 186 1810 761 1028 2301 Fragments arranged by size: 2301 1810 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 2605 4422 4507 5578 2208 Size: 397 1817 85 1071 2991 Fragments arranged by size: 2991 1817 1071 397 85 BsmI GAATG_Cn' Cuts at: 3872 3965 3872 Size: 93 6268 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2605 3398 3680 4422 4507 5578 Size: 399 418 397 793 282 742 85 1071 Cuts at: 5578 1391 Size: 2174 Fragments arranged by size: 2174 1071 793 742 418 399 397 282 85 BspEI T'CCGG_A Cuts at: 6024 6024 Size: 6361 BspHI T'CATG_A Cuts at: 4250 5980 4250 Size: 1730 4631 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3716 844 Size: 2872 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4248 1394 Size: 429 2425 3507 Fragments arranged by size: 3507 2425 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3108 3174 4856 5030 65 Size: 1183 1860 66 1682 174 1396 Fragments arranged by size: 1860 1682 1396 1183 174 66 BsrFI r'CCGG_y Cuts at: 1700 1810 5005 5192 1700 Size: 110 3195 187 2869 Fragments arranged by size: 3195 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2586 2626 2862 3093 96 Size: 2490 40 236 231 3364 Fragments arranged by size: 3364 2490 236 231 40 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6361 BssSI C'ACGA_G Cuts at: 4421 5433 4421 Size: 1012 5349 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6361 BstBI TT'CG_AA Cuts at: 5165 5165 Size: 6361 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6361 BstYI r'GATC_y Cuts at: 1972 2287 2698 2757 3691 4058 4443 4460 5912 Size: 315 411 59 934 367 385 17 1452 Cuts at: 5912 5998 6010 6356 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 934 411 385 367 346 315 86 59 17 12 BtgI C'CryG_G Cuts at: 513 1065 2703 513 Size: 552 1638 4171 Fragments arranged by size: 4171 1638 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2254 487 Size: 683 56 1028 4594 Fragments arranged by size: 4594 1028 683 56 BtsI GCAGTG_nn' Cuts at: 2779 3877 4671 4691 2779 Size: 1098 794 20 4449 Fragments arranged by size: 4449 1098 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6361 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2038 2073 3595 3630 529 Size: 35 1474 35 1522 35 3260 Fragments arranged by size: 3260 1522 1474 35 35 35 DraI TTT'AAA Cuts at: 2739 3665 2739 Size: 926 5435 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2488 5368 809 Size: 507 1172 2880 1802 Fragments arranged by size: 2880 1802 1172 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3808 4697 6326 Size: 54 1425 50 275 18 1978 889 1629 Cuts at: 6326 8 Size: 43 Fragments arranged by size: 1978 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3808 1537 Size: 275 1996 4090 Fragments arranged by size: 4090 1996 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3200 3233 4291 1353 Size: 1847 33 1058 3423 Fragments arranged by size: 3423 1847 1058 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3637 4948 5322 5468 387 Size: 1064 138 2048 1311 374 146 1280 Fragments arranged by size: 2048 1311 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2389 2581 4424 5021 5808 6076 1239 Size: 393 757 192 1843 597 787 268 1524 Fragments arranged by size: 1843 1524 787 757 597 393 268 192 EcoO109I rG'GnC_Cy Cuts at: 2471 2808 2471 Size: 337 6024 EcoRI G'AATT_C Cuts at: 3670 4048 3670 Size: 378 5983 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2402 2473 3094 2402 Size: 71 621 5669 Fragments arranged by size: 5669 621 71 FspI TGC'GCA Cuts at: 4867 4867 Size: 6361 HaeII r_GCGC'y Cuts at: 1678 5508 1678 Size: 3830 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2357 2389 2648 2680 3279 Size: 32 135 32 785 32 259 32 599 Cuts at: 3279 3311 5002 5034 5076 5108 1373 Size: 32 1691 32 42 32 2626 Fragments arranged by size: 2626 1691 785 599 259 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3237 3705 3951 669 Size: 988 281 1299 468 246 3079 Fragments arranged by size: 3079 1299 988 468 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6361 HpaI GTT'AAC Cuts at: 3237 3951 3237 Size: 714 5647 KpnI G_GTAC'C Cuts at: 3686 3686 Size: 6361 MfeI C'AATT_G Cuts at: 2522 3366 3960 2522 Size: 844 594 4923 Fragments arranged by size: 4923 844 594 MluI A'CGCG_T Cuts at: 6237 6237 Size: 6361 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2559 2689 3044 3526 5475 5659 1305 Size: 1254 130 355 482 1949 184 2007 Fragments arranged by size: 2007 1949 1254 482 355 184 130 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4539 Fragments arranged by size: 4539 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4319 4678 4837 518 Size: 648 3153 359 159 2042 Fragments arranged by size: 3153 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2087 3279 3777 4454 5602 5847 1606 Size: 481 1192 498 677 1148 245 2120 Fragments arranged by size: 2120 1192 1148 677 498 481 245 NcoI C'CATG_G Cuts at: 513 1065 2703 513 Size: 552 1638 4171 Fragments arranged by size: 4171 1638 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6361 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6361 NotI GC'GGCC_GC Cuts at: 3808 3808 Size: 6361 NsiI A_TGCA'T Cuts at: 2010 3565 2010 Size: 1555 4806 NspI r_CATG'y Cuts at: 1132 1229 3337 3567 3719 5264 1132 Size: 97 2108 230 152 1545 2229 Fragments arranged by size: 2229 2108 1545 230 152 97 PasI CC'CwG_GG Cuts at: 1348 2339 1348 Size: 991 5370 PciI A'CATG_T Cuts at: 3333 5260 3333 Size: 1927 4434 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6361 PfoI T'CCnGG_A Cuts at: 2753 2753 Size: 6361 PmeI GTTT'AAAC Cuts at: 3665 3665 Size: 6361 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4430 4462 5943 5975 4430 Size: 32 1481 32 4816 Fragments arranged by size: 4816 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2471 2471 Size: 6361 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6361 PsiI TTA'TAA Cuts at: 3495 3931 3495 Size: 436 5925 PstI C_TGCA'G Cuts at: 830 3713 830 Size: 2883 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6361 PvuII CAG'CTG Cuts at: 2087 3279 2087 Size: 1192 5169 SalI G'TCGA_C Cuts at: 1936 3703 1936 Size: 1767 4594 SanDI GG'GwC_CC Cuts at: 2471 2471 Size: 6361 SapI GCTCTTCn'nnn_ Cuts at: 3200 3200 Size: 6361 SbfI CC_TGCA'GG Cuts at: 3713 3713 Size: 6361 ScaI AGT'ACT Cuts at: 4609 6321 4609 Size: 1712 4649 SfcI C'TryA_G Cuts at: 826 1045 3027 3709 4844 5525 5716 826 Size: 219 1982 682 1135 681 191 1471 Fragments arranged by size: 1982 1471 1135 682 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6361 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6361 SmaI CCC'GGG Cuts at: 1872 3688 1872 Size: 1816 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2299 4465 5366 5628 820 Size: 197 926 356 2166 901 262 1553 Fragments arranged by size: 2166 1553 926 901 356 262 197 SnaBI TAC'GTA Cuts at: 493 4152 493 Size: 3659 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6361 SphI G_CATG'C Cuts at: 1132 3567 3719 1132 Size: 2435 152 3774 Fragments arranged by size: 3774 2435 152 SspI AAT'ATT Cuts at: 5 52 4285 5 Size: 47 4233 2081 Fragments arranged by size: 4233 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6361 StyI C'CwwG_G Cuts at: 513 1065 2074 2703 3800 513 Size: 552 1009 629 1097 3074 Fragments arranged by size: 3074 1097 1009 629 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2817 3378 3461 4388 4405 4558 4743 Size: 1255 1237 561 83 927 17 153 185 Cuts at: 4743 5188 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1237 927 561 445 185 153 83 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2586 2626 2862 Size: 274 80 33 51 1443 609 40 236 Cuts at: 2862 3093 4607 6319 96 Size: 231 1514 1712 138 Fragments arranged by size: 1712 1514 1443 609 274 236 231 138 80 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2601 2633 6321 6353 2601 Size: 32 3688 32 2609 Fragments arranged by size: 3688 2609 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6361 XbaI T'CTAG_A Cuts at: 3697 3697 Size: 6361 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2933 3450 2933 Size: 517 5844 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6361 XmaI C'CCGG_G Cuts at: 1870 3686 1870 Size: 1816 4545 XmnI GAAnn'nnTTC Cuts at: 2456 4490 2456 Size: 2034 4327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4169 276 Size: 53 83 186 936 2635 2468 Fragments arranged by size: 2635 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI