(Linear) (Six-base) MAPSORT of: KIEE5683.seq Check: 5470 from: 1 to: 1656 KIEE5683.orf With 182 enzymes: SgfI * June 11, 2014 14:42 .. AclI AA'CG_TT Cuts at: 0 1546 1656 Size: 1546 110 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 576 1656 Size: 384 192 1080 Fragments arranged by size: 1080 384 192 AflII C'TTAA_G Cuts at: 0 294 1656 Size: 294 1362 AflIII A'CryG_T Cuts at: 0 507 1328 1656 Size: 507 821 328 Fragments arranged by size: 821 507 328 ApoI r'AATT_y Cuts at: 0 907 1205 1656 Size: 907 298 451 Fragments arranged by size: 907 451 298 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 522 555 1656 Size: 522 33 1101 Fragments arranged by size: 1101 522 33 BanI G'GyrC_C Cuts at: 0 148 1656 Size: 148 1508 BbsI GAAGACnn'nnnn_ Cuts at: 0 1293 1656 Size: 1293 363 BbvCI CC'TCA_GC Cuts at: 0 763 1656 Size: 763 893 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 942 976 986 1020 1656 Size: 942 34 10 34 636 Fragments arranged by size: 942 636 34 34 10 BciVI GTATCCnnnnn_n' Cuts at: 0 34 1656 Size: 34 1622 BfrBI ATG'CAT Cuts at: 0 3 1558 1656 Size: 3 1555 98 Fragments arranged by size: 1555 98 3 BglI GCCn_nnn'nGGC Cuts at: 0 157 1656 Size: 157 1499 BlpI GC'TnA_GC Cuts at: 0 964 1656 Size: 964 692 Bme1580I G_kGCm'C Cuts at: 0 1393 1656 Size: 1393 263 BmrI ACTGGGnnnn_n' Cuts at: 0 610 1656 Size: 610 1046 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 79 1656 Size: 79 1577 Bpu10I CC'TnA_GC Cuts at: 0 763 1656 Size: 763 893 BsaI GGTCTCn'nnnn_ Cuts at: 0 1255 1656 Size: 1255 401 BsaAI yAC'GTr Cuts at: 0 510 1656 Size: 510 1146 BsaBI GATnn'nnATC Cuts at: 0 394 1656 Size: 394 1262 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 850 880 1656 Size: 850 30 776 Fragments arranged by size: 850 776 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 55 1210 1656 Size: 55 1155 446 Fragments arranged by size: 1155 446 55 BseYI C'CCAG_C Cuts at: 0 654 1381 1656 Size: 654 727 275 Fragments arranged by size: 727 654 275 BsiHKAI G_wGCw'C Cuts at: 0 203 600 1656 Size: 203 397 1056 Fragments arranged by size: 1056 397 203 Bsp1286I G_dGCh'C Cuts at: 0 203 600 1393 1656 Size: 203 397 793 263 Fragments arranged by size: 793 397 263 203 BsrDI GCAATG_nn' Cuts at: 0 1103 1169 1656 Size: 1103 66 487 Fragments arranged by size: 1103 487 66 BsrGI T'GTAC_A Cuts at: 0 581 621 857 1088 1656 Size: 581 40 236 231 568 Fragments arranged by size: 581 568 236 231 40 BstYI r'GATC_y Cuts at: 0 282 693 752 1656 Size: 282 411 59 904 Fragments arranged by size: 904 411 282 59 BtgI C'CryG_G Cuts at: 0 698 1656 Size: 698 958 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 249 1656 Size: 249 1407 BtsI GCAGTG_nn' Cuts at: 0 774 1656 Size: 774 882 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 33 68 1590 1625 1656 Size: 33 35 1522 35 31 Fragments arranged by size: 1522 35 35 33 31 DraI TTT'AAA Cuts at: 0 734 1656 Size: 734 922 DrdI GACnn_nn'nnGTC Cuts at: 0 483 1656 Size: 483 1173 EarI CTCTTCn'nnn_ Cuts at: 0 1195 1228 1656 Size: 1195 33 428 Fragments arranged by size: 1195 428 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1632 1656 Size: 1632 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 384 576 1656 Size: 384 192 1080 Fragments arranged by size: 1080 384 192 EcoO109I rG'GnC_Cy Cuts at: 0 466 803 1656 Size: 466 337 853 Fragments arranged by size: 853 466 337 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 397 468 1089 1656 Size: 397 71 621 567 Fragments arranged by size: 621 567 397 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 352 384 643 675 1274 1306 1656 Size: 352 32 259 32 599 32 350 Fragments arranged by size: 599 352 350 259 32 32 32 HincII GTy'rAC Cuts at: 0 1232 1656 Size: 1232 424 HpaI GTT'AAC Cuts at: 0 1232 1656 Size: 1232 424 MfeI C'AATT_G Cuts at: 0 517 1361 1656 Size: 517 844 295 Fragments arranged by size: 844 517 295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 554 684 1039 1521 1656 Size: 554 130 355 482 135 Fragments arranged by size: 554 482 355 135 130 MspA1I CmG'CkG Cuts at: 0 82 1274 1656 Size: 82 1192 382 Fragments arranged by size: 1192 382 82 NcoI C'CATG_G Cuts at: 0 698 1656 Size: 698 958 NsiI A_TGCA'T Cuts at: 0 5 1560 1656 Size: 5 1555 96 Fragments arranged by size: 1555 96 5 NspI r_CATG'y Cuts at: 0 1332 1562 1656 Size: 1332 230 94 Fragments arranged by size: 1332 230 94 PasI CC'CwG_GG Cuts at: 0 334 1656 Size: 334 1322 PciI A'CATG_T Cuts at: 0 1328 1656 Size: 1328 328 PfoI T'CCnGG_A Cuts at: 0 748 1656 Size: 748 908 PpuMI rG'GwC_Cy Cuts at: 0 466 1656 Size: 466 1190 PsiI TTA'TAA Cuts at: 0 1490 1656 Size: 1490 166 PvuII CAG'CTG Cuts at: 0 82 1274 1656 Size: 82 1192 382 Fragments arranged by size: 1192 382 82 SanDI GG'GwC_CC Cuts at: 0 466 1656 Size: 466 1190 SapI GCTCTTCn'nnn_ Cuts at: 0 1195 1656 Size: 1195 461 SfcI C'TryA_G Cuts at: 0 1022 1656 Size: 1022 634 SmlI C'TyrA_G Cuts at: 0 294 1656 Size: 294 1362 SphI G_CATG'C Cuts at: 0 1562 1656 Size: 1562 94 StyI C'CwwG_G Cuts at: 0 69 698 1656 Size: 69 629 958 Fragments arranged by size: 958 629 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 812 1373 1456 1656 Size: 812 561 83 200 Fragments arranged by size: 812 561 200 83 TatI w'GTAC_w Cuts at: 0 581 621 857 1088 1656 Size: 581 40 236 231 568 Fragments arranged by size: 581 568 236 231 40 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 596 628 1656 Size: 596 32 1028 Fragments arranged by size: 1028 596 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 928 1445 1656 Size: 928 517 211 Fragments arranged by size: 928 517 211 XmnI GAAnn'nnTTC Cuts at: 0 451 1656 Size: 451 1205 Enzymes that do cut: AclI AcuI AflII AflIII ApoI BaeI BanI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmrI BplI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BseYI BsiHKAI Bsp1286I BsrDI BsrGI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EarI EciI Eco57MI EcoO109I FalI Hin4I HincII HpaI MfeI MmeI MspA1I NcoI NsiI NspI PasI PciI PfoI PpuMI PsiI PvuII SanDI SapI SfcI SmlI SphI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BanII BbeI BclI BglII BmgBI BmtI BpmI BpuEI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraIII EaeI EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI