################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII AccI AccIII AcsI AflII AflIII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspHI AspI AviII AvrII BalI BanII BbrPI BcgI BclI BfaI BfrI BglI BlnI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MaeI MluI MluNI MroI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 1207 GGTACC G^GTACC AclI 886 AACGTT AA^CGTT AhdI 682 GACCCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 352 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 831 CCTAAGG CC^T[ACGT]AGG Asp718I 1207 GGTACC G^GTACC AspEI 682 GACCCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 610 GGATCC G^GATCC BbeI 211 GGCGCC GGCGC^C BbsI 1464 GTCTTC GAAGAC(2/6) BbvCI 916 GCTGAGG CCTCAGC(-5/-2) BciVI 619 GTATCC GTATCC(6/5) BglII 422 AGATCT A^GATCT BlpI 814 GCTAAGC GC^T[ACGT]AGC BpmI 806 CTCCAG CTGGAG(16/14) Bpu1102I 814 GCTAAGC GC^T[ACGT]AGC BpuAI 1464 GTCTTC GAAGAC(2/6) BsaAI 463 TACGTG [CT]AC^GT[GA] BsaWI 719 ACCGGA [AT]^CCGG[AT] BseRI 151 GAGGAG GAGGAG(10/8) BsgI 529 GTGCAG GTGCAG(16/14) BsiEI 51 CGGCCG CG[GA][CT]^CG BsrBI 66 GAGCGG CCGCTC(-3/-3) BsrFI 33 GCCGGC [GA]^CCGG[CT] BstAPI 1328 GCACTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 831 CCTAAGG CC^T[ACGT]AGG CelII 814 GCTAAGC GC^T[ACGT]AGC Cfr10I 33 GCCGGC [GA]^CCGG[CT] DraII 1256 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 820 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 51 CGGCCG C^GGCCG Eam1105I 682 GACCCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 51 CGGCCG C^GGCCG Eco52I 51 CGGCCG C^GGCCG Eco81I 831 CCTAAGG CC^T[ACGT]AGG EcoNI 658 CCTCGGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1256 GGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 767 CCTTGG C^C[AT][AT]GG KasI 211 GGCGCC G^GCGCC KpnI 1207 GGTACC GGTAC^C MaeIII 751 GTCAC ^GT[ACGT]AC MfeI 1553 CAATTG C^AATTG MunI 1553 CAATTG C^AATTG NaeI 33 GCCGGC GCC^GGC NarI 211 GGCGCC GG^CGCC NgoMIV 33 GCCGGC G^CCGGC PpuMI 1256 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 886 AACGTT AA^CGTT PstI 204 CTGCAG CTGCA^G PvuII 931 CAGCTG CAG^CTG SfcI 204 CTGCAG C^T[GA][CT]AG SfoI 211 GGCGCC GGC^GCC SmaI 228 CCCGGG CCC^GGG SmlI 120 CTTGAG C^T[CT][GA]AG SphI 1073 GCATGC GCATG^C StyI 767 CCTTGG C^C[AT][AT]GG TaqI 319 TCGA T^CGA Tsp45I 751 GTCAC ^GT[GC]AC TthHB8I 319 TCGA T^CGA XmaCI 228 CCCGGG C^CCGGG XmaI 228 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BmyI 223 GGGCAC G[AGT]GC[ACT]^C 304 GTGCCC BsaBI 372 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 402 GATGCCCATC BsaI 716 GAGACC GGTCTC(1/5) 1246 GAGACC Bsp1286I 223 GGGCAC G[AGT]GC[ACT]^C 304 GTGCCC BsrDI 948 CATTGC GCAATG(2/0) 1314 CATTGC BssHII 242 GCGCGC G^CGCGC 543 GCGCGC BstXI 324 CCAGAAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1130 CCACCAGGCTGG DsaI 851 CCACGG C^C[GA][CT]GG 1078 CCACGG EaeI 51 CGGCCG [CT]^GGCC[GA] 770 TGGCCG EarI 1121 CTCTTC CTCTTC(1/4) 1234 CTCTTC HaeII 157 GGCGCT [GA]GCGC^[CT] 211 GGCGCC Ksp632I 1121 CTCTTC CTCTTC(1/4) 1234 CTCTTC MamI 372 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 402 GATGCCCATC MseI 890 TTAA T^TAA 1060 TTAA MspA1I 61 CAGCGG C[AC]G^C[GT]G 931 CAGCTG NspI 578 ACATGC [GA]CATG^[CT] 1073 GCATGC Tru9I 890 TTAA T^TAA 1060 TTAA Tsp509I 1300 AATT ^AATT 1554 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaI AvaII BanI BbvI BcnI BsaHI BsaJI BsiYI BslI BsmAI BsmFI BsoBI BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MflI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI TseI TspRI XhoII