(Circular) (Six-base) MAPSORT of: pFN21AB5932.seq Check: 9816 from: 1 to: 6319 pFN21AB5932 6319 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2296 2296 Size: 6319 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4129 278 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 3212 3640 3212 Size: 428 5891 AclI AA'CG_TT Cuts at: 1563 2892 4446 4819 1563 Size: 1329 1554 373 3063 Fragments arranged by size: 3063 1554 1329 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2372 3226 3456 4382 5766 6034 1632 Size: 740 854 230 926 1384 268 1917 Fragments arranged by size: 1917 1384 926 854 740 268 230 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 2692 5048 2692 Size: 2356 3963 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6319 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2579 1184 Size: 1395 4924 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3086 3118 3086 Size: 32 6287 AlwNI CAG_nnn'CTG Cuts at: 2362 4062 5634 2362 Size: 1700 1572 3047 Fragments arranged by size: 3047 1700 1572 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 3628 3854 4006 6115 1493 Size: 2135 226 152 2109 1697 Fragments arranged by size: 2135 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2233 2300 2312 2910 3644 1870 Size: 73 290 67 12 598 734 4545 Fragments arranged by size: 4545 734 598 290 73 67 12 BamHI G'GATC_C Cuts at: 2615 3649 4016 2615 Size: 1034 367 4918 Fragments arranged by size: 4918 1034 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 2229 2308 3001 3212 Size: 325 217 627 429 13 79 693 211 Cuts at: 3212 3419 3640 5095 618 Size: 207 221 1455 1842 Fragments arranged by size: 1842 1455 693 627 429 325 221 217 211 207 79 13 BanII G_rGCy'C Cuts at: 1391 3638 1391 Size: 2247 4072 BbeI G_GCGC'C Cuts at: 2220 2220 Size: 6319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3462 928 Size: 971 1563 3785 Fragments arranged by size: 3785 1563 971 BbvCI CC'TCA_GC Cuts at: 2922 2922 Size: 6319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2635 4211 5421 2635 Size: 1576 1210 3533 Fragments arranged by size: 3533 1576 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4930 136 Size: 107 122 71 631 605 3258 1525 Fragments arranged by size: 3258 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2427 6314 2427 Size: 3887 2432 BlpI GC'TnA_GC Cuts at: 2820 3736 2820 Size: 916 5403 Bme1580I G_kGCm'C Cuts at: 1790 2232 2313 4380 5536 1790 Size: 442 81 2067 1156 2573 Fragments arranged by size: 2573 2067 1156 442 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2615 3104 3614 Size: 416 53 83 399 24 1198 489 510 Cuts at: 3614 4050 5008 442 Size: 436 958 1753 Fragments arranged by size: 1753 1198 958 510 489 436 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2338 2338 Size: 6319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2796 4979 1239 Size: 1557 2183 2579 Fragments arranged by size: 2579 2183 1557 Bpu10I CC'TnA_GC Cuts at: 2922 2922 Size: 6319 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2146 4444 5309 5607 2146 Size: 2298 865 298 2858 Fragments arranged by size: 2858 2298 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2715 3245 4982 882 Size: 617 1216 530 1737 2219 Fragments arranged by size: 2219 1737 1216 617 530 BsaAI yAC'GTr Cuts at: 493 1204 2470 4110 493 Size: 711 1266 1640 2702 Fragments arranged by size: 2702 1640 1266 711 BsaBI GATnn'nnATC Cuts at: 2381 2411 6313 2381 Size: 30 3902 2387 Fragments arranged by size: 3902 2387 30 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 2217 2478 2514 Size: 53 83 186 936 603 81 261 36 Cuts at: 2514 4126 4508 5143 275 Size: 1612 382 635 1451 Fragments arranged by size: 1612 1451 936 635 603 382 261 186 83 81 53 36 BsaWI w'CCGG_w Cuts at: 2724 4752 5150 5424 5571 5982 2724 Size: 2028 398 274 147 411 3061 Fragments arranged by size: 3061 2028 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6094 Fragments arranged by size: 6094 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 1186 Size: 985 5334 BseYI C'CCAG_C Cuts at: 2033 2225 2362 2371 2938 2957 5522 6018 2033 Size: 192 137 9 567 19 2565 496 2334 Fragments arranged by size: 2565 2334 567 496 192 137 19 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2555 1163 Size: 573 819 4927 Fragments arranged by size: 4927 819 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2059 3769 4530 5558 1540 Size: 275 186 58 1710 761 1028 2301 Fragments arranged by size: 2301 1710 1028 761 275 186 58 BsiHKAI G_wGCw'C Cuts at: 4380 4465 5536 4380 Size: 85 1071 5163 Fragments arranged by size: 5163 1071 85 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2313 3638 4380 4465 5536 1391 Size: 399 442 81 1325 742 85 1071 2174 Fragments arranged by size: 2174 1325 1071 742 442 399 85 81 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2296 2304 2514 2910 3674 844 Size: 1452 8 210 396 764 3489 Fragments arranged by size: 3489 1452 764 396 210 8 BsrBI CCG'CTC Cuts at: 1394 1823 2073 4206 1394 Size: 429 250 2133 3507 Fragments arranged by size: 3507 2133 429 250 BsrDI GCAATG_nn' Cuts at: 65 1248 2952 3318 4814 4988 65 Size: 1183 1704 366 1496 174 1396 Fragments arranged by size: 1704 1496 1396 1183 366 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 4963 5150 1700 Size: 110 228 2925 187 2869 Fragments arranged by size: 2925 2869 228 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6319 BssHII G'CGCG_C Cuts at: 1925 2247 2548 1925 Size: 322 301 5696 Fragments arranged by size: 5696 322 301 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 3339 794 Size: 2545 3774 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6319 BstXI CCAn_nnnn'nTGG Cuts at: 2336 3142 2336 Size: 806 5513 BstYI r'GATC_y Cuts at: 1972 2427 2615 3187 3649 4016 4401 4418 5870 Size: 455 188 572 462 367 385 17 1452 Cuts at: 5870 5956 5968 6314 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 572 462 455 385 367 346 188 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2837 2837 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 2004 2856 3083 513 Size: 552 939 852 227 3749 Fragments arranged by size: 3749 939 852 552 227 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2682 487 Size: 683 56 1456 4124 Fragments arranged by size: 4124 1456 683 56 BtsI GCAGTG_nn' Cuts at: 3333 3835 4629 4649 3333 Size: 502 794 20 5003 Fragments arranged by size: 5003 794 502 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DraIII CAC_nnn'GTG Cuts at: 2830 2830 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 2775 3615 Size: 54 1425 50 275 18 226 719 840 Cuts at: 3615 3766 4655 6284 8 Size: 151 889 1629 43 Fragments arranged by size: 1629 1425 889 840 719 275 226 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2056 3766 1537 Size: 275 244 1710 4090 Fragments arranged by size: 4090 1710 275 244 EarI CTCTTCn'nnn_ Cuts at: 1353 3132 3245 4249 1353 Size: 1779 113 1004 3423 Fragments arranged by size: 3423 1779 1004 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2462 2640 4906 5280 5426 387 Size: 1064 138 873 178 2266 374 146 1280 Fragments arranged by size: 2266 1280 1064 873 374 178 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2372 2796 3226 3456 4382 4979 5766 Size: 393 740 424 430 230 926 597 787 Cuts at: 5766 6034 1239 Size: 268 1524 Fragments arranged by size: 1524 926 787 740 597 430 424 393 268 230 EcoNI CCTnn'n_nnAGG Cuts at: 2667 2667 Size: 6319 EcoO109I rG'GnC_Cy Cuts at: 3262 3262 Size: 6319 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2166 2220 5466 1678 Size: 488 54 3246 2531 Fragments arranged by size: 3246 2531 488 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2678 2710 2962 2994 4960 Size: 32 135 32 1106 32 252 32 1966 Cuts at: 4960 4992 5034 5066 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1966 1106 252 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KasI G'GCGC_C Cuts at: 2216 2216 Size: 6319 KpnI G_GTAC'C Cuts at: 3216 3644 3216 Size: 428 5891 MfeI C'AATT_G Cuts at: 3558 3918 3558 Size: 360 5959 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2913 5433 5617 1305 Size: 1608 2520 184 2007 Fragments arranged by size: 2520 2007 1608 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4497 Fragments arranged by size: 4497 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4277 4636 4795 518 Size: 648 3111 359 159 2042 Fragments arranged by size: 3111 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2068 2938 3735 4412 5560 5805 1606 Size: 462 870 797 677 1148 245 2120 Fragments arranged by size: 2120 1148 870 797 677 462 245 NaeI GCC'GGC Cuts at: 2040 2040 Size: 6319 NarI GG'CG_CC Cuts at: 2217 2217 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4828 Fragments arranged by size: 4828 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 2038 2038 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 2587 3082 3677 5222 1132 Size: 97 1358 495 595 1545 2229 Fragments arranged by size: 2229 1545 1358 595 495 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6319 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6319 PfoI T'CCnGG_A Cuts at: 3157 3157 Size: 6319 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3262 3262 Size: 6319 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PstI C_TGCA'G Cuts at: 830 2213 3671 830 Size: 1383 1458 3478 Fragments arranged by size: 3478 1458 1383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 PvuII CAG'CTG Cuts at: 2938 2938 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SfcI C'TryA_G Cuts at: 826 1045 2209 3667 4802 5483 5674 826 Size: 219 1164 1458 1135 681 191 1471 Fragments arranged by size: 1471 1458 1164 1135 681 219 191 SfoI GGC'GCC Cuts at: 2218 2218 Size: 6319 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 2235 3646 1872 Size: 363 1411 4545 Fragments arranged by size: 4545 1411 363 SmlI C'TyrA_G Cuts at: 820 1017 1943 2125 4423 5324 5586 820 Size: 197 926 182 2298 901 262 1553 Fragments arranged by size: 2298 1553 926 901 262 197 182 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3082 3677 1132 Size: 1950 595 3774 Fragments arranged by size: 3774 1950 595 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2004 2772 3758 513 Size: 552 939 768 986 3074 Fragments arranged by size: 3074 986 939 768 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2534 2625 4346 4363 4516 4701 5146 Size: 1255 954 91 1721 17 153 185 445 Cuts at: 5146 325 Size: 1498 Fragments arranged by size: 1721 1498 1255 954 445 185 153 91 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2580 3289 4565 Size: 274 80 33 51 1443 603 709 1276 Cuts at: 4565 6277 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1276 709 603 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6279 6311 6279 Size: 32 6287 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 2233 3644 1870 Size: 363 1411 4545 Fragments arranged by size: 4545 1411 363 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4127 276 Size: 53 83 186 936 2593 2468 Fragments arranged by size: 2593 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI