################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbsI BcgI BciVI BfrI BglI BglII BlnI BpmI BpuAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SmlI SnaBI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 19 GGTACC G^GTACC AcsI 618 AAATTC [GA]^AATT[CT] AcyI 3 GGCGTC G[GA]^CG[CT]C AflIII 587 ACATGT A^C[GA][CT]GT AlwNI 730 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 889 CCTGAGG CC^T[ACGT]AGG ApoI 618 AAATTC [GA]^AATT[CT] Asp700I 181 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 19 GGTACC G^GTACC AvaII 897 GGACC G^G[AT]CC BanI 19 GGTACC G^G[CT][GA]CC BbrPI 346 CACGTG CAC^GTG BbvCI 253 CCTCAGC CCTCAGC(-5/-2) BfaI 800 CTAG C^TAG BlpI 690 GCTCAGC GC^T[ACGT]AGC BmyI 392 GGGCAC G[AGT]GC[ACT]^C Bpu1102I 690 GCTCAGC GC^T[ACGT]AGC BsaAI 346 CACGTG [CT]AC^GT[GA] BsaHI 3 GGCGTC G[GA]^CG[CT]C BsgI 115 GTGCAG GTGCAG(16/14) BsmFI 6 GTCCC GGGAC(10/14) Bsp1286I 392 GGGCAC G[AGT]GC[ACT]^C BspLU11I 587 ACATGT A^CATGT BsrBI 883 CCGCTC CCGCTC(-3/-3) BsrDI 954 CATTGC GCAATG(2/0) BstYI 478 GGATCT [GA]^GATC[CT] Bsu36I 889 CCTGAGG CC^T[ACGT]AGG CelII 690 GCTCAGC GC^T[ACGT]AGC DraII 896 AGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 889 CCTGAGG CC^T[ACGT]AGG EcoO109I 896 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 474 CCAAGG C^C[AT][AT]GG HaeII 860 AGCGCC [GA]GCGC^[CT] Hin1I 3 GGCGTC G[GA]^CG[CT]C HphI 575 TCACC GGTGA(8/7) KpnI 19 GGTACC GGTAC^C MaeI 800 CTAG C^TAG MaeII 347 ACGT A^CGT MflI 478 GGATCT [GA]^GATC[CT] MslI 783 CACATACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 665 CAGCTG C[AC]G^C[GT]G NaeI 55 GCCGGC GCC^GGC NgoMIV 55 GCCGGC G^CCGGC PmaCI 346 CACGTG CAC^GTG PmlI 346 CACGTG CAC^GTG PpuMI 896 AGGACCT [GA]G^G[AT]CC[CT] PvuII 665 CAGCTG CAG^CTG SfcI 727 CTACAG C^T[GA][CT]AG SgrAI 54 CGCCGGCG C[GA]^CCGG[CT]G SmaI 389 CCCGGG CCC^GGG SpeI 799 ACTAGT A^CTAGT SphI 423 GCATGC GCATG^C StyI 474 CCAAGG C^C[AT][AT]GG TspRI 999 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 478 GGATCT [GA]^GATC[CT] XmaCI 389 CCCGGG C^CCGGG XmaI 389 CCCGGG C^CCGGG XmnI 181 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 20 GTAC GT^AC 585 GTAC AlwI 457 GATCC GGATC(4/5) 478 GGATC AvaI 90 CTCGGG C^[CT]CG[GA]G 389 CCCGGG BclI 336 TGATCA T^GATCA 629 TGATCA BcnI 389 CCCGG CC^[GC]GG 390 CCGGG BseRI 872 GAGGAG GAGGAG(10/8) 892 GAGGAG BsiYI 639 CCTAATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 888 CCCTGAGGAGG BslI 639 CCTAATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 888 CCCTGAGGAGG BsoBI 90 CTCGGG C^[CT]CG[GA]G 389 CCCGGG BsrFI 22 ACCGGC [GA]^CCGG[CT] 55 GCCGGC CfoI 59 GCGC GCG^C 861 GCGC Cfr10I 22 ACCGGC [GA]^CCGG[CT] 55 GCCGGC Cfr13I 897 GGACC G^G[ACGT]CC 1029 GGCCC FauI 8 CCCGC CCCGC(4/6) 882 CCCGC FbaI 336 TGATCA T^GATCA 629 TGATCA HgaI 4 GCGTC GACGC(5/10) 837 GCGTC HhaI 59 GCGC GCG^C 861 GCGC HinP1I 59 GCGC G^CGC 861 GCGC MaeIII 385 GTTAC ^GT[ACGT]AC 416 GTTAC NciI 389 CCCGG CC^[GC]GG 390 CCGGG NlaIV 19 GGTACC GG[ACGT]^[ACGT]CC 314 GGAGCC NspI 423 GCATGC [GA]CATG^[CT] 587 ACATGT RsaI 20 GTAC GT^AC 585 GTAC SapI 1020 GCTCTTC GCTCTTC(1/4) 1036 GCTCTTC Sau96I 897 GGACC G^G[ACGT]CC 1029 GGCCC SfaNI 271 GATGC GCATC(5/9) 309 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BbvI BsaJI BssKI BstF5I BstNI Cac8I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII PleI Sau3AI ScrFI TfiI Tru9I TseI Tsp509I