(Linear) (Six-base) MAPSORT of: KIEE5453.seq Check: 4675 from: 1 to: 1056 KIEE5453.orf With 182 enzymes: SgfI * January 29, 2014 12:01 .. Acc65I G'GTAC_C Cuts at: 0 19 1056 Size: 19 1037 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 572 1056 Size: 200 372 484 Fragments arranged by size: 484 372 200 AflIII A'CryG_T Cuts at: 0 587 1056 Size: 587 469 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 869 901 1056 Size: 869 32 155 Fragments arranged by size: 869 155 32 AlwNI CAG_nnn'CTG Cuts at: 0 735 1056 Size: 735 321 ApoI r'AATT_y Cuts at: 0 618 1056 Size: 618 438 AvaI C'yCGr_G Cuts at: 0 90 389 1056 Size: 90 299 667 Fragments arranged by size: 667 299 90 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 811 844 1056 Size: 811 33 212 Fragments arranged by size: 811 212 33 BanI G'GyrC_C Cuts at: 0 19 1056 Size: 19 1037 BbvCI CC'TCA_GC Cuts at: 0 254 1056 Size: 254 802 BclI T'GATC_A Cuts at: 0 336 629 1056 Size: 336 293 427 Fragments arranged by size: 427 336 293 BlpI GC'TnA_GC Cuts at: 0 691 1056 Size: 691 365 Bme1580I G_kGCm'C Cuts at: 0 396 1056 Size: 396 660 Bpu10I CC'TnA_GC Cuts at: 0 254 1056 Size: 254 802 BsaAI yAC'GTr Cuts at: 0 348 1056 Size: 348 708 BsaHI Gr'CG_yC Cuts at: 0 4 1056 Size: 4 1052 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 869 886 899 916 1056 Size: 869 17 13 17 140 Fragments arranged by size: 869 140 17 17 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 887 907 1056 Size: 887 20 149 Fragments arranged by size: 887 149 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 1056 Size: 136 920 Bsp1286I G_dGCh'C Cuts at: 0 396 1056 Size: 396 660 BsrBI CCG'CTC Cuts at: 0 885 1056 Size: 885 171 BsrDI GCAATG_nn' Cuts at: 0 953 1056 Size: 953 103 BsrFI r'CCGG_y Cuts at: 0 22 55 1056 Size: 22 33 1001 Fragments arranged by size: 1001 33 22 BstYI r'GATC_y Cuts at: 0 478 1056 Size: 478 578 Bsu36I CC'TnA_GG Cuts at: 0 890 1056 Size: 890 166 BtsI GCAGTG_nn' Cuts at: 0 998 1056 Size: 998 58 EarI CTCTTCn'nnn_ Cuts at: 0 45 916 1027 1043 1056 Size: 45 871 111 16 13 Fragments arranged by size: 871 111 45 16 13 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 572 1056 Size: 200 372 484 Fragments arranged by size: 484 372 200 EcoO109I rG'GnC_Cy Cuts at: 0 897 1056 Size: 897 159 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 214 241 1056 Size: 214 27 815 Fragments arranged by size: 815 214 27 HaeII r_GCGC'y Cuts at: 0 864 1056 Size: 864 192 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 886 918 1056 Size: 886 32 138 Fragments arranged by size: 886 138 32 KpnI G_GTAC'C Cuts at: 0 23 1056 Size: 23 1033 MslI CAynn'nnrTG Cuts at: 0 787 1056 Size: 787 269 MspA1I CmG'CkG Cuts at: 0 667 1056 Size: 667 389 NaeI GCC'GGC Cuts at: 0 57 1056 Size: 57 999 NgoMIV G'CCGG_C Cuts at: 0 55 1056 Size: 55 1001 NspI r_CATG'y Cuts at: 0 427 591 1056 Size: 427 164 465 Fragments arranged by size: 465 427 164 PciI A'CATG_T Cuts at: 0 587 1056 Size: 587 469 PfoI T'CCnGG_A Cuts at: 0 398 1056 Size: 398 658 PmlI CAC'GTG Cuts at: 0 348 1056 Size: 348 708 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 869 901 1056 Size: 869 32 155 Fragments arranged by size: 869 155 32 PpuMI rG'GwC_Cy Cuts at: 0 897 1056 Size: 897 159 PvuII CAG'CTG Cuts at: 0 667 1056 Size: 667 389 SapI GCTCTTCn'nnn_ Cuts at: 0 1027 1043 1056 Size: 1027 16 13 Fragments arranged by size: 1027 16 13 SfcI C'TryA_G Cuts at: 0 727 1056 Size: 727 329 SgrAI Cr'CCGG_yG Cuts at: 0 55 1056 Size: 55 1001 SmaI CCC'GGG Cuts at: 0 391 1056 Size: 391 665 SpeI A'CTAG_T Cuts at: 0 799 1056 Size: 799 257 SphI G_CATG'C Cuts at: 0 427 1056 Size: 427 629 StyI C'CwwG_G Cuts at: 0 474 1056 Size: 474 582 TatI w'GTAC_w Cuts at: 0 584 1056 Size: 584 472 XmaI C'CCGG_G Cuts at: 0 389 1056 Size: 389 667 XmnI GAAnn'nnTTC Cuts at: 0 185 1056 Size: 185 871 Enzymes that do cut: Acc65I AcuI AflIII AloI AlwNI ApoI AvaI BaeI BanI BbvCI BclI BlpI Bme1580I Bpu10I BsaAI BsaHI BsaXI BseRI BsgI Bsp1286I BsrBI BsrDI BsrFI BstYI Bsu36I BtsI EarI Eco57MI EcoO109I FalI HaeII Hin4I KpnI MslI MspA1I NaeI NgoMIV NspI PciI PfoI PmlI PpiI PpuMI PvuII SapI SfcI SgrAI SmaI SpeI SphI StyI TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BamHI BanII BbeI BbsI BcgI BciVI BfrBI BglI BglII BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MmeI MscI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SmlI SnaBI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI ZraI