################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AflII AgeI AhdI Aor51HI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BbsI BbvCI BcgI BclI BfrI BlnI BpuAI BsaBI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KpnI KspI MamI MluNI MscI NaeI NgoMIV NheI NotI NsiI NspV PacI PflFI PinAI PmeI PshAI PvuI RcaI RsrII SacII SalI SapI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 2275 GTCTAC GT^[AC][GT]AC AccIII 44 TCCGGA T^CCGGA AclI 70 AACGTT AA^CGTT AflIII 2792 ACGCGT A^C[GA][CT]GT ApaI 2630 GGGCCC GGGCC^C AseI 1480 ATTAAT AT^TAAT AsnI 1480 ATTAAT AT^TAAT AvaI 1542 CTCGAG C^[CT]CG[GA]G BbeI 2604 GGCGCC GGCGC^C BbrPI 3427 CACGTG CAC^GTG BciVI 2646 GTATCC GTATCC(6/5) BcnI 672 CCCGG CC^[GC]GG BglI 2545 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 750 AGATCT A^GATCT BsaAI 3427 CACGTG [CT]AC^GT[GA] BsaI 3255 GAGACC GGTCTC(1/5) BseAI 44 TCCGGA T^CCGGA BsoBI 1542 CTCGAG C^[CT]CG[GA]G Bsp1407I 3336 TGTACA T^GTACA BspEI 44 TCCGGA T^CCGGA BsrGI 3336 TGTACA T^GTACA DraIII 539 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG FauI 2439 GCGGG CCCGC(4/6) HgaI 2481 GACGC GACGC(5/10) HincII 883 GTCAAC GT[CT]^[GA]AC HindII 883 GTCAAC GT[CT]^[GA]AC KasI 2604 GGCGCC G^GCGCC MluI 2792 ACGCGT A^CGCGT MroI 44 TCCGGA T^CCGGA MslI 1059 CACTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 2604 GGCGCC GG^CGCC NciI 672 CCCGG CC^[GC]GG NruI 1867 TCGCGA TCG^CGA NspI 225 GCATGT [GA]CATG^[CT] PaeR7I 1542 CTCGAG C^TCGAG PmaCI 3427 CACGTG CAC^GTG PmlI 3427 CACGTG CAC^GTG PshBI 1480 ATTAAT AT^TAAT Psp1406I 70 AACGTT AA^CGTT SacI 1539 GAGCTC GAGCT^C SbfI 2595 CCTGCAGG CCTGCA^GG SfoI 2604 GGCGCC GGC^GCC Sse8387I 2595 CCTGCAGG CCTGCA^GG SspBI 3336 TGTACA T^GTACA XcmI 3747 CCATGGTGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1542 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 2481 GACGCC G[GA]^CG[CT]C 2604 GGCGCC Alw44I 1823 GTGCAC G^TGCAC 3691 GTGCAC AocI 467 CCTCAGG CC^T[ACGT]AGG 3163 CCTCAGG ApaLI 1823 GTGCAC G^TGCAC 3691 GTGCAC Asp700I 1560 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3188 GAAATAGTTC BsaHI 2481 GACGCC G[GA]^CG[CT]C 2604 GGCGCC BsaWI 44 TCCGGA [AT]^CCGG[AT] 2713 TCCGGT BsmI 2766 GAATGC GAATGC(1/-1) 3546 GCATTC BsrDI 2408 GCAATG GCAATG(2/0) 2948 GCAATG Bsu36I 467 CCTCAGG CC^T[ACGT]AGG 3163 CCTCAGG DraI 703 TTTAAA TTT^AAA 2835 TTTAAA DrdI 2266 GACTTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2481 GACGCCTGTGTC DsaI 1644 CCATGG C^C[GA][CT]GG 3747 CCATGG Eco81I 467 CCTCAGG CC^T[ACGT]AGG 3163 CCTCAGG HaeII 2604 GGCGCC [GA]GCGC^[CT] 2891 AGCGCC Hin1I 2481 GACGCC G[GA]^CG[CT]C 2604 GGCGCC MfeI 555 CAATTG C^AATTG 2730 CAATTG MunI 555 CAATTG C^AATTG 2730 CAATTG NcoI 1644 CCATGG C^CATGG 3747 CCATGG NdeI 3736 CATATG CA^TATG 3777 CATATG PpuMI 949 GGGACCT [GA]G^G[AT]CC[CT] 3621 AGGTCCC ScaI 3172 AGTACT AGT^ACT 3661 AGTACT StuI 931 AGGCCT AGG^CCT 2830 AGGCCT XbaI 1306 TCTAGA T^CTAGA 3528 TCTAGA XmnI 1560 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3188 GAAATAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII