(Circular) (Six-base) MAPSORT of: pFN21AE3441.seq Check: 2369 from: 1 to: 8587 pFN21AE3441 8587 bp With 182 enzymes: SgfI * March 28, 2012 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2959 4002 2959 Size: 1043 7544 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6397 278 Size: 53 83 186 936 4861 2468 Fragments arranged by size: 4861 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4281 5930 1937 Size: 2344 1649 4594 Fragments arranged by size: 4594 2344 1649 Acc65I G'GTAC_C Cuts at: 5908 5908 Size: 8587 AclI AA'CG_TT Cuts at: 1563 2076 6714 7087 1563 Size: 513 4638 373 3063 Fragments arranged by size: 4638 3063 513 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2598 2684 2961 3092 3150 4258 4901 5232 Size: 966 86 277 131 58 1108 643 331 Cuts at: 5232 6650 8034 8302 1632 Size: 1418 1384 268 1917 Fragments arranged by size: 1917 1418 1384 1108 966 643 331 277 268 131 86 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8390 AflIII A'CryG_T Cuts at: 1203 4797 7486 8463 1203 Size: 3594 2689 977 1327 Fragments arranged by size: 3594 2689 1327 977 AgeI A'CCGG_T Cuts at: 7418 7418 Size: 8587 AhdI GACnn_n'nnGTC Cuts at: 7316 7316 Size: 8587 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8587 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4129 4671 1184 Size: 2945 542 5100 Fragments arranged by size: 5100 2945 542 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4378 4410 5209 5241 4378 Size: 32 799 32 7724 Fragments arranged by size: 7724 799 32 32 AlwNI CAG_nnn'CTG Cuts at: 2104 2396 4325 4696 6330 7902 2104 Size: 292 1929 371 1634 1572 2789 Fragments arranged by size: 2789 1929 1634 1572 371 292 ApaI G_GGCC'C Cuts at: 4639 4639 Size: 8587 ApaLI G'TGCA_C Cuts at: 3828 5696 6644 7800 3828 Size: 1868 948 1156 4615 Fragments arranged by size: 4615 1868 1156 948 ApoI r'AATT_y Cuts at: 1493 2150 3050 3353 5028 5896 6122 6274 8383 Size: 657 900 303 1675 868 226 152 2109 Cuts at: 8383 1493 Size: 1697 Fragments arranged by size: 2109 1697 1675 900 868 657 303 226 152 AseI AT'TA_AT Cuts at: 160 1032 3486 7141 160 Size: 872 2454 3655 1606 Fragments arranged by size: 3655 2454 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8587 AvaI C'yCGr_G Cuts at: 1870 1943 3547 5912 1870 Size: 73 1604 2365 4545 Fragments arranged by size: 4545 2365 1604 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3703 3736 4030 4063 3703 Size: 33 294 33 8227 Fragments arranged by size: 8227 294 33 33 BamHI G'GATC_C Cuts at: 5917 6284 5917 Size: 367 8220 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2680 4499 4609 5579 5602 Size: 325 217 627 893 1819 110 970 23 Cuts at: 5602 5908 7363 618 Size: 306 1455 1842 Fragments arranged by size: 1842 1819 1455 970 893 627 325 306 217 110 23 BanII G_rGCy'C Cuts at: 1391 2415 2579 3479 3548 4263 4371 4639 5906 Size: 1024 164 900 69 715 108 268 1267 Cuts at: 5906 1391 Size: 4072 Fragments arranged by size: 4072 1267 1024 900 715 268 164 108 69 BbeI G_GCGC'C Cuts at: 4613 4613 Size: 8587 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7616 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6778 6812 1903 Size: 34 50 34 4757 34 3678 Fragments arranged by size: 4757 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4662 6479 7689 4662 Size: 1817 1210 5560 Fragments arranged by size: 5560 1817 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8587 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4556 7198 136 Size: 107 122 71 631 605 2884 2642 1525 Fragments arranged by size: 2884 2642 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2755 8582 2755 Size: 5827 2760 BlpI GC'TnA_GC Cuts at: 2817 4923 5091 6004 2817 Size: 2106 168 913 5400 Fragments arranged by size: 5400 2106 913 168 Bme1580I G_kGCm'C Cuts at: 1790 3291 3832 4639 5700 6648 7804 1790 Size: 1501 541 807 1061 948 1156 2573 Fragments arranged by size: 2573 1501 1156 1061 948 807 541 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8587 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2310 2573 4684 Size: 416 53 83 399 24 893 263 2111 Cuts at: 4684 6318 7276 442 Size: 1634 958 1753 Fragments arranged by size: 2111 1753 1634 958 893 416 399 263 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8587 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3193 3193 Size: 8587 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2373 2507 2676 3237 3559 4187 4281 Size: 887 247 134 169 561 322 628 94 Cuts at: 4281 5202 5404 7247 1239 Size: 921 202 1843 2579 Fragments arranged by size: 2579 1843 921 887 628 561 322 247 202 169 134 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2436 5446 5655 6712 7577 7875 2436 Size: 3010 209 1057 865 298 3148 Fragments arranged by size: 3148 3010 1057 865 298 209 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5254 7250 882 Size: 617 3755 1996 2219 Fragments arranged by size: 3755 2219 1996 617 BsaAI yAC'GTr Cuts at: 493 1204 5434 6378 493 Size: 711 4230 944 2702 Fragments arranged by size: 4230 2702 944 711 BsaBI GATnn'nnATC Cuts at: 8581 8581 Size: 8587 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4487 4610 6394 6776 Size: 53 83 186 936 2954 123 1784 382 Cuts at: 6776 7411 275 Size: 635 1451 Fragments arranged by size: 2954 1784 1451 936 635 382 186 123 83 53 BsaWI w'CCGG_w Cuts at: 2049 4718 7020 7418 7692 7839 8250 2049 Size: 2669 2302 398 274 147 411 2386 Fragments arranged by size: 2669 2386 2302 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2321 2351 4305 4335 5567 Size: 30 165 30 918 30 1954 30 1232 Cuts at: 5567 5597 1178 Size: 30 4168 Fragments arranged by size: 4168 1954 1232 918 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2415 3136 3920 4271 4897 4973 5002 5484 Size: 1229 721 784 351 626 76 29 482 Cuts at: 5484 1186 Size: 4289 Fragments arranged by size: 4289 1229 784 721 626 482 351 76 29 BseYI C'CCAG_C Cuts at: 2024 2396 4644 5039 7790 8286 2024 Size: 372 2248 395 2751 496 2325 Fragments arranged by size: 2751 2325 2248 496 395 372 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2449 2876 2987 3180 3449 3827 4153 Size: 573 713 427 111 193 269 378 326 Cuts at: 4153 5797 1163 Size: 1644 3953 Fragments arranged by size: 3953 1644 713 573 427 378 326 269 193 111 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6037 6798 7826 1540 Size: 275 186 4036 761 1028 2301 Fragments arranged by size: 4036 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3548 3832 4108 4173 5700 6648 6733 7804 3548 Size: 284 276 65 1527 948 85 1071 4331 Fragments arranged by size: 4331 1527 1071 948 284 276 85 65 BsmI GAATG_Cn' Cuts at: 4777 5551 6098 6191 4777 Size: 774 547 93 7173 Fragments arranged by size: 7173 774 547 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2415 2579 3291 3479 3548 3832 4108 Size: 399 625 164 712 188 69 284 276 Cuts at: 4108 4173 4263 4371 4639 5700 5906 6648 6733 Size: 65 90 108 268 1061 206 742 85 Cuts at: 6733 7804 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1061 742 712 625 399 284 276 268 206 188 164 108 90 85 69 65 BspEI T'CCGG_A Cuts at: 2049 8250 2049 Size: 6201 2386 BspHI T'CATG_A Cuts at: 6476 8206 6476 Size: 1730 6857 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2959 4002 5272 5942 844 Size: 2115 1043 1270 670 3489 Fragments arranged by size: 3489 2115 1270 1043 670 BsrBI CCG'CTC Cuts at: 1394 1823 6474 1394 Size: 429 4651 3507 Fragments arranged by size: 4651 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4420 4960 7082 7256 65 Size: 1183 3172 540 2122 174 1396 Fragments arranged by size: 3172 2122 1396 1183 540 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7231 7418 1700 Size: 110 5421 187 2869 Fragments arranged by size: 5421 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 5341 96 Size: 5245 3342 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8587 BssSI C'ACGA_G Cuts at: 6647 7659 6647 Size: 1012 7575 BstAPI GCAn_nnn'nTGC Cuts at: 794 5146 5579 5784 794 Size: 4352 433 205 3597 Fragments arranged by size: 4352 3597 433 205 BstBI TT'CG_AA Cuts at: 7391 7391 Size: 8587 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8587 BstYI r'GATC_y Cuts at: 1972 2100 2239 2755 5185 5917 6284 6669 6686 Size: 128 139 516 2430 732 367 385 17 Cuts at: 6686 8138 8224 8236 8582 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2430 1977 1452 732 516 385 367 346 139 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2473 5169 2473 Size: 2696 5891 BtgI C'CryG_G Cuts at: 513 1065 3649 5752 513 Size: 552 2584 2103 3348 Fragments arranged by size: 3348 2584 2103 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7848 Fragments arranged by size: 7848 683 56 BtsI GCAGTG_nn' Cuts at: 3432 4186 6103 6897 6917 3432 Size: 754 1917 794 20 5102 Fragments arranged by size: 5102 1917 794 754 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8587 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2704 2739 4783 4818 529 Size: 35 2140 35 2044 35 4298 Fragments arranged by size: 4298 2140 2044 35 35 35 DraI TTT'AAA Cuts at: 2710 4842 5891 2710 Size: 2132 1049 5406 Fragments arranged by size: 5406 2132 1049 DraIII CAC_nnn'GTG Cuts at: 2549 2549 Size: 8587 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4277 4492 7594 809 Size: 507 2961 215 3102 1802 Fragments arranged by size: 3102 2961 1802 507 215 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 6034 6923 8552 Size: 54 1425 50 275 18 4204 889 1629 Cuts at: 8552 8 Size: 43 Fragments arranged by size: 4204 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6034 1537 Size: 275 4222 4090 Fragments arranged by size: 4222 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4074 4218 4334 4350 6517 1353 Size: 2721 144 116 16 2167 3423 Fragments arranged by size: 3423 2721 2167 144 116 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4635 4797 7174 7548 7694 387 Size: 1064 138 3046 162 2377 374 146 1280 Fragments arranged by size: 3046 2377 1280 1064 374 162 146 138 EcoICRI GAG'CTC Cuts at: 3546 3546 Size: 8587 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2373 2507 2598 2676 2684 2961 Size: 393 494 247 134 91 78 8 277 Cuts at: 2961 3092 3150 3237 3559 4187 4258 4281 4901 Size: 131 58 87 322 628 71 23 620 Cuts at: 4901 5202 5232 5404 6650 7247 8034 8302 1239 Size: 301 30 172 1246 597 787 268 1524 Fragments arranged by size: 1524 1246 787 628 620 597 494 393 322 301 277 268 247 172 134 131 91 87 78 71 58 30 23 8 EcoO109I rG'GnC_Cy Cuts at: 2955 4635 4636 5627 5836 2955 Size: 1680 1 991 209 5706 Fragments arranged by size: 5706 1680 991 209 1 EcoRI G'AATT_C Cuts at: 5896 6274 5896 Size: 378 8209 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 2314 2800 3586 5696 2281 Size: 33 486 786 2110 5172 Fragments arranged by size: 5172 2110 786 486 33 FspI TGC'GCA Cuts at: 7093 7093 Size: 8587 HaeII r_GCGC'y Cuts at: 1678 4613 4900 7734 1678 Size: 2935 287 2834 2531 Fragments arranged by size: 2935 2834 2531 287 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 3530 3562 4142 Size: 32 135 32 581 32 1345 32 580 Cuts at: 4142 4174 4504 4536 4976 5008 5149 5181 5224 Size: 32 330 32 440 32 141 32 43 Cuts at: 5224 5256 7228 7260 7302 7334 1373 Size: 32 1972 32 42 32 2626 Fragments arranged by size: 2626 1972 1345 581 580 440 330 141 135 43 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2890 5931 6177 669 Size: 988 281 952 3041 246 3079 Fragments arranged by size: 3079 3041 988 952 281 246 HindIII A'AGCT_T Cuts at: 748 3132 3464 4817 748 Size: 2384 332 1353 4518 Fragments arranged by size: 4518 2384 1353 332 HpaI GTT'AAC Cuts at: 6177 6177 Size: 8587 KasI G'GCGC_C Cuts at: 4609 4609 Size: 8587 KpnI G_GTAC'C Cuts at: 5912 5912 Size: 8587 MfeI C'AATT_G Cuts at: 2560 4735 6186 2560 Size: 2175 1451 4961 Fragments arranged by size: 4961 2175 1451 MluI A'CGCG_T Cuts at: 4797 8463 4797 Size: 3666 4921 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5491 7701 7885 1305 Size: 4186 2210 184 2007 Fragments arranged by size: 4186 2210 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6765 Fragments arranged by size: 6765 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3068 6545 6904 7063 518 Size: 648 1902 3477 359 159 2042 Fragments arranged by size: 3477 2042 1902 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2393 3045 3369 4113 4145 4616 5507 6003 Size: 787 652 324 744 32 471 891 496 Cuts at: 6003 6680 7828 8073 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 891 787 744 677 652 496 471 324 245 32 NarI GG'CG_CC Cuts at: 4610 4610 Size: 8587 NcoI C'CATG_G Cuts at: 513 1065 3649 5752 513 Size: 552 2584 2103 3348 Fragments arranged by size: 3348 2584 2103 552 NdeI CA'TA_TG Cuts at: 387 5742 5783 387 Size: 5355 41 3191 Fragments arranged by size: 5355 3191 41 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8587 NotI GC'GGCC_GC Cuts at: 6034 6034 Size: 8587 NruI TCG'CGA Cuts at: 3874 3874 Size: 8587 NspI r_CATG'y Cuts at: 1132 1229 2234 5945 7490 1132 Size: 97 1005 3711 1545 2229 Fragments arranged by size: 3711 2229 1545 1005 97 PasI CC'CwG_GG Cuts at: 1348 5062 1348 Size: 3714 4873 PciI A'CATG_T Cuts at: 7486 7486 Size: 8587 PflMI CCAn_nnn'nTGG Cuts at: 1108 2104 2396 5119 5327 1108 Size: 996 292 2723 208 4368 Fragments arranged by size: 4368 2723 996 292 208 PfoI T'CCnGG_A Cuts at: 2103 5188 2103 Size: 3085 5502 PmeI GTTT'AAAC Cuts at: 5891 5891 Size: 8587 PmlI CAC'GTG Cuts at: 5434 5434 Size: 8587 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6656 6688 8169 8201 6656 Size: 32 1481 32 7042 Fragments arranged by size: 7042 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2955 5627 2955 Size: 2672 5915 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8587 PsiI TTA'TAA Cuts at: 6157 6157 Size: 8587 PspOMI G'GGCC_C Cuts at: 4635 4635 Size: 8587 PspXI vC'TCGA_Gb Cuts at: 3547 3547 Size: 8587 PstI C_TGCA'G Cuts at: 830 2161 2200 2919 4605 5939 830 Size: 1331 39 719 1686 1334 3478 Fragments arranged by size: 3478 1686 1334 1331 719 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8587 PvuII CAG'CTG Cuts at: 2393 3369 4113 4145 4616 5507 2393 Size: 976 744 32 471 891 5473 Fragments arranged by size: 5473 976 891 744 471 32 SacI G_AGCT'C Cuts at: 3548 3548 Size: 8587 SalI G'TCGA_C Cuts at: 1936 5929 1936 Size: 3993 4594 SbfI CC_TGCA'GG Cuts at: 4605 5939 4605 Size: 1334 7253 ScaI AGT'ACT Cuts at: 5179 5668 6835 8547 5179 Size: 489 1167 1712 5219 Fragments arranged by size: 5219 1712 1167 489 SfcI C'TryA_G Cuts at: 826 1045 2112 2157 2196 2523 2915 4096 4209 Size: 219 1067 45 39 327 392 1181 113 Cuts at: 4209 4601 5244 5935 7070 7751 7942 826 Size: 392 643 691 1135 681 191 1471 Fragments arranged by size: 1471 1181 1135 1067 691 681 643 392 392 327 219 191 113 45 39 SfoI GGC'GCC Cuts at: 4611 4611 Size: 8587 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8587 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8587 SmaI CCC'GGG Cuts at: 1872 5914 1872 Size: 4042 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2451 3547 5425 5670 6691 7592 Size: 197 926 508 1096 1878 245 1021 901 Cuts at: 7592 7854 820 Size: 262 1553 Fragments arranged by size: 1878 1553 1096 1021 926 901 508 262 245 197 SnaBI TAC'GTA Cuts at: 493 6378 493 Size: 5885 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8587 SphI G_CATG'C Cuts at: 1132 5945 1132 Size: 4813 3774 SspI AAT'ATT Cuts at: 5 52 6511 5 Size: 47 6459 2081 Fragments arranged by size: 6459 2081 47 StuI AGG'CCT Cuts at: 1517 2938 4837 1517 Size: 1421 1899 5267 Fragments arranged by size: 5267 1899 1421 StyI C'CwwG_G Cuts at: 513 1065 3022 3649 3775 4590 4677 5586 5752 Size: 552 1957 627 126 815 87 909 166 Cuts at: 5752 6026 513 Size: 274 3074 Fragments arranged by size: 3074 1957 909 815 627 552 274 166 126 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3132 4403 4478 4517 5289 6614 6631 Size: 1255 1552 1271 75 39 772 1325 17 Cuts at: 6631 6784 6969 7414 325 Size: 153 185 445 1498 Fragments arranged by size: 1552 1498 1325 1271 1255 772 445 185 153 75 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3925 5177 5253 Size: 274 80 33 51 1443 1948 1252 76 Cuts at: 5253 5341 5666 6833 8545 96 Size: 88 325 1167 1712 138 Fragments arranged by size: 1948 1712 1443 1252 1167 325 274 138 88 80 76 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8547 8579 8547 Size: 32 8555 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8587 XbaI T'CTAG_A Cuts at: 3311 5533 5923 3311 Size: 2222 390 5975 Fragments arranged by size: 5975 2222 390 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5759 5759 Size: 8587 XhoI C'TCGA_G Cuts at: 1943 3547 1943 Size: 1604 6983 XmaI C'CCGG_G Cuts at: 1870 5912 1870 Size: 4042 4545 XmnI GAAnn'nnTTC Cuts at: 3569 5197 6716 3569 Size: 1628 1519 5440 Fragments arranged by size: 5440 1628 1519 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6395 276 Size: 53 83 186 936 4861 2468 Fragments arranged by size: 4861 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI FspAI NaeI NgoMIV NsiI PacI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI