(Linear) (Six-base) MAPSORT of: KIEE3441.seq Check: 8006 from: 1 to: 3882 KIEE3441.orf With 182 enzymes: SgfI * March 22, 2012 16:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 954 1997 3882 Size: 954 1043 1885 Fragments arranged by size: 1885 1043 954 AccI GT'mk_AC Cuts at: 0 2276 3882 Size: 2276 1606 AclI AA'CG_TT Cuts at: 0 71 3882 Size: 71 3811 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 593 679 956 1087 1145 2253 2896 3227 Size: 593 86 277 131 58 1108 643 331 Cuts at: 3227 3882 Size: 655 Fragments arranged by size: 1108 655 643 593 331 277 131 86 58 AflIII A'CryG_T Cuts at: 0 2792 3882 Size: 2792 1090 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2124 2666 3882 Size: 2124 542 1216 Fragments arranged by size: 2124 1216 542 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2373 2405 3204 3236 3882 Size: 2373 32 799 32 646 Fragments arranged by size: 2373 799 646 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 99 391 2320 2691 3882 Size: 99 292 1929 371 1191 Fragments arranged by size: 1929 1191 371 292 99 ApaI G_GGCC'C Cuts at: 0 2634 3882 Size: 2634 1248 ApaLI G'TGCA_C Cuts at: 0 1823 3691 3882 Size: 1823 1868 191 Fragments arranged by size: 1868 1823 191 ApoI r'AATT_y Cuts at: 0 145 1045 1348 3023 3882 Size: 145 900 303 1675 859 Fragments arranged by size: 1675 900 859 303 145 AseI AT'TA_AT Cuts at: 0 1481 3882 Size: 1481 2401 AvaI C'yCGr_G Cuts at: 0 1542 3882 Size: 1542 2340 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1698 1731 2025 2058 3882 Size: 1698 33 294 33 1824 Fragments arranged by size: 1824 1698 294 33 33 BanI G'GyrC_C Cuts at: 0 675 2494 2604 3574 3597 3882 Size: 675 1819 110 970 23 285 Fragments arranged by size: 1819 970 675 285 110 23 BanII G_rGCy'C Cuts at: 0 410 574 1474 1543 2258 2366 2634 3882 Size: 410 164 900 69 715 108 268 1248 Fragments arranged by size: 1248 900 715 410 268 164 108 69 BbeI G_GCGC'C Cuts at: 0 2608 3882 Size: 2608 1274 BciVI GTATCCnnnnn_n' Cuts at: 0 2657 3882 Size: 2657 1225 BglI GCCn_nnn'nGGC Cuts at: 0 2551 3882 Size: 2551 1331 BglII A'GATC_T Cuts at: 0 750 3882 Size: 750 3132 BlpI GC'TnA_GC Cuts at: 0 812 2918 3086 3882 Size: 812 2106 168 796 Fragments arranged by size: 2106 812 796 168 Bme1580I G_kGCm'C Cuts at: 0 1286 1827 2634 3695 3882 Size: 1286 541 807 1061 187 Fragments arranged by size: 1286 1061 807 541 187 BmrI ACTGGGnnnn_n' Cuts at: 0 305 568 2679 3882 Size: 305 263 2111 1203 Fragments arranged by size: 2111 1203 305 263 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1188 3882 Size: 1188 2694 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 368 502 671 1232 1554 2182 2276 Size: 121 247 134 169 561 322 628 94 Cuts at: 2276 3197 3399 3882 Size: 921 202 483 Fragments arranged by size: 921 628 561 483 322 247 202 169 134 121 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 431 3441 3650 3882 Size: 431 3010 209 232 Fragments arranged by size: 3010 431 232 209 BsaI GGTCTCn'nnnn_ Cuts at: 0 3249 3882 Size: 3249 633 BsaAI yAC'GTr Cuts at: 0 3429 3882 Size: 3429 453 BsaHI Gr'CG_yC Cuts at: 0 2482 2605 3882 Size: 2482 123 1277 Fragments arranged by size: 2482 1277 123 BsaWI w'CCGG_w Cuts at: 0 44 2713 3882 Size: 44 2669 1169 Fragments arranged by size: 2669 1169 44 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 316 346 2300 2330 3562 3592 3882 Size: 316 30 1954 30 1232 30 290 Fragments arranged by size: 1954 1232 316 290 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 410 1131 1915 2266 2892 2968 2997 3479 Size: 410 721 784 351 626 76 29 482 Cuts at: 3479 3882 Size: 403 Fragments arranged by size: 784 721 626 482 410 403 351 76 29 BseYI C'CCAG_C Cuts at: 0 19 391 2639 3034 3882 Size: 19 372 2248 395 848 Fragments arranged by size: 2248 848 395 372 19 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 444 871 982 1175 1444 1822 2148 3792 Size: 444 427 111 193 269 378 326 1644 Cuts at: 3792 3882 Size: 90 Fragments arranged by size: 1644 444 427 378 326 269 193 111 90 BsiHKAI G_wGCw'C Cuts at: 0 1543 1827 2103 2168 3695 3882 Size: 1543 284 276 65 1527 187 Fragments arranged by size: 1543 1527 284 276 187 65 BsmI GAATG_Cn' Cuts at: 0 2772 3546 3882 Size: 2772 774 336 Fragments arranged by size: 2772 774 336 Bsp1286I G_dGCh'C Cuts at: 0 410 574 1286 1474 1543 1827 2103 2168 Size: 410 164 712 188 69 284 276 65 Cuts at: 2168 2258 2366 2634 3695 3882 Size: 90 108 268 1061 187 Fragments arranged by size: 1061 712 410 284 276 268 188 187 164 108 90 69 65 BspEI T'CCGG_A Cuts at: 0 44 3882 Size: 44 3838 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 954 1997 3267 3882 Size: 954 1043 1270 615 Fragments arranged by size: 1270 1043 954 615 BsrDI GCAATG_nn' Cuts at: 0 2415 2955 3882 Size: 2415 540 927 Fragments arranged by size: 2415 927 540 BsrGI T'GTAC_A Cuts at: 0 3336 3882 Size: 3336 546 BstAPI GCAn_nnn'nTGC Cuts at: 0 3141 3574 3779 3882 Size: 3141 433 205 103 Fragments arranged by size: 3141 433 205 103 BstYI r'GATC_y Cuts at: 0 95 234 750 3180 3882 Size: 95 139 516 2430 702 Fragments arranged by size: 2430 702 516 139 95 Bsu36I CC'TnA_GG Cuts at: 0 468 3164 3882 Size: 468 2696 718 Fragments arranged by size: 2696 718 468 BtgI C'CryG_G Cuts at: 0 1644 3747 3882 Size: 1644 2103 135 Fragments arranged by size: 2103 1644 135 BtsI GCAGTG_nn' Cuts at: 0 1427 2181 3882 Size: 1427 754 1701 Fragments arranged by size: 1701 1427 754 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 699 734 2778 2813 3882 Size: 699 35 2044 35 1069 Fragments arranged by size: 2044 1069 699 35 35 DraI TTT'AAA Cuts at: 0 705 2837 3882 Size: 705 2132 1045 Fragments arranged by size: 2132 1045 705 DraIII CAC_nnn'GTG Cuts at: 0 544 3882 Size: 544 3338 DrdI GACnn_nn'nnGTC Cuts at: 0 2272 2487 3882 Size: 2272 215 1395 Fragments arranged by size: 2272 1395 215 EarI CTCTTCn'nnn_ Cuts at: 0 2069 2213 2329 2345 3882 Size: 2069 144 116 16 1537 Fragments arranged by size: 2069 1537 144 116 16 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2630 2792 3882 Size: 2630 162 1090 Fragments arranged by size: 2630 1090 162 EcoICRI GAG'CTC Cuts at: 0 1541 3882 Size: 1541 2341 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 368 502 593 671 679 956 1087 Size: 121 247 134 91 78 8 277 131 Cuts at: 1087 1145 1232 1554 2182 2253 2276 2896 3197 Size: 58 87 322 628 71 23 620 301 Cuts at: 3197 3227 3399 3882 Size: 30 172 483 Fragments arranged by size: 628 620 483 322 301 277 247 172 134 131 121 91 87 78 71 58 30 23 8 EcoO109I rG'GnC_Cy Cuts at: 0 950 2630 2631 3622 3831 3882 Size: 950 1680 1 991 209 51 Fragments arranged by size: 1680 991 950 209 51 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 276 309 795 1581 3691 3882 Size: 276 33 486 786 2110 191 Fragments arranged by size: 2110 786 486 276 191 33 HaeII r_GCGC'y Cuts at: 0 2608 2895 3882 Size: 2608 287 987 Fragments arranged by size: 2608 987 287 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 148 180 1525 1557 2137 2169 2499 2531 Size: 148 32 1345 32 580 32 330 32 Cuts at: 2531 2971 3003 3144 3176 3219 3251 3882 Size: 440 32 141 32 43 32 631 Fragments arranged by size: 1345 631 580 440 330 148 141 43 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 885 3882 Size: 885 2997 HindIII A'AGCT_T Cuts at: 0 1127 1459 2812 3882 Size: 1127 332 1353 1070 Fragments arranged by size: 1353 1127 1070 332 KasI G'GCGC_C Cuts at: 0 2604 3882 Size: 2604 1278 MfeI C'AATT_G Cuts at: 0 555 2730 3882 Size: 555 2175 1152 Fragments arranged by size: 2175 1152 555 MluI A'CGCG_T Cuts at: 0 2792 3882 Size: 2792 1090 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 3486 3882 Size: 3486 396 MslI CAynn'nnrTG Cuts at: 0 1063 3882 Size: 1063 2819 MspA1I CmG'CkG Cuts at: 0 388 1040 1364 2108 2140 2611 3502 3882 Size: 388 652 324 744 32 471 891 380 Fragments arranged by size: 891 744 652 471 388 380 324 32 NarI GG'CG_CC Cuts at: 0 2605 3882 Size: 2605 1277 NcoI C'CATG_G Cuts at: 0 1644 3747 3882 Size: 1644 2103 135 Fragments arranged by size: 2103 1644 135 NdeI CA'TA_TG Cuts at: 0 3737 3778 3882 Size: 3737 41 104 Fragments arranged by size: 3737 104 41 NruI TCG'CGA Cuts at: 0 1869 3882 Size: 1869 2013 NspI r_CATG'y Cuts at: 0 229 3882 Size: 229 3653 PasI CC'CwG_GG Cuts at: 0 3057 3882 Size: 3057 825 PflMI CCAn_nnn'nTGG Cuts at: 0 99 391 3114 3322 3882 Size: 99 292 2723 208 560 Fragments arranged by size: 2723 560 292 208 99 PfoI T'CCnGG_A Cuts at: 0 98 3183 3882 Size: 98 3085 699 Fragments arranged by size: 3085 699 98 PmlI CAC'GTG Cuts at: 0 3429 3882 Size: 3429 453 PpuMI rG'GwC_Cy Cuts at: 0 950 3622 3882 Size: 950 2672 260 Fragments arranged by size: 2672 950 260 PspOMI G'GGCC_C Cuts at: 0 2630 3882 Size: 2630 1252 PspXI vC'TCGA_Gb Cuts at: 0 1542 3882 Size: 1542 2340 PstI C_TGCA'G Cuts at: 0 156 195 914 2600 3882 Size: 156 39 719 1686 1282 Fragments arranged by size: 1686 1282 719 156 39 PvuII CAG'CTG Cuts at: 0 388 1364 2108 2140 2611 3502 3882 Size: 388 976 744 32 471 891 380 Fragments arranged by size: 976 891 744 471 388 380 32 SacI G_AGCT'C Cuts at: 0 1543 3882 Size: 1543 2339 SbfI CC_TGCA'GG Cuts at: 0 2600 3882 Size: 2600 1282 ScaI AGT'ACT Cuts at: 0 3174 3663 3882 Size: 3174 489 219 Fragments arranged by size: 3174 489 219 SfcI C'TryA_G Cuts at: 0 107 152 191 518 910 2091 2204 2596 Size: 107 45 39 327 392 1181 113 392 Cuts at: 2596 3239 3882 Size: 643 643 Fragments arranged by size: 1181 643 643 392 392 327 113 107 45 39 SfoI GGC'GCC Cuts at: 0 2606 3882 Size: 2606 1276 SmlI C'TyrA_G Cuts at: 0 446 1542 3420 3665 3882 Size: 446 1096 1878 245 217 Fragments arranged by size: 1878 1096 446 245 217 StuI AGG'CCT Cuts at: 0 933 2832 3882 Size: 933 1899 1050 Fragments arranged by size: 1899 1050 933 StyI C'CwwG_G Cuts at: 0 1017 1644 1770 2585 2672 3581 3747 3882 Size: 1017 627 126 815 87 909 166 135 Fragments arranged by size: 1017 909 815 627 166 135 126 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1127 2398 2473 2512 3284 3882 Size: 1127 1271 75 39 772 598 Fragments arranged by size: 1271 1127 772 598 75 39 TatI w'GTAC_w Cuts at: 0 1920 3172 3248 3336 3661 3882 Size: 1920 1252 76 88 325 221 Fragments arranged by size: 1920 1252 325 221 88 76 XbaI T'CTAG_A Cuts at: 0 1306 3528 3882 Size: 1306 2222 354 Fragments arranged by size: 2222 1306 354 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 3754 3882 Size: 3754 128 XhoI C'TCGA_G Cuts at: 0 1542 3882 Size: 1542 2340 XmnI GAAnn'nnTTC Cuts at: 0 1564 3192 3882 Size: 1564 1628 690 Fragments arranged by size: 1628 1564 690 Enzymes that do cut: AarI AccI AclI AcuI AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII BbeI BciVI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrGI BstAPI BstYI Bsu36I BtgI BtsI CspCI DraI DraIII DrdI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII KasI MfeI MluI MmeI MslI MspA1I NarI NcoI NdeI NruI NspI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SbfI ScaI SfcI SfoI SmlI StuI StyI TaqII TatI XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AgeI AhdI AleI AscI AsiSI AvrII BamHI BbsI BbvCI BcgI BclI BfrBI BmgBI BmtI Bpu10I BsaBI BsiEI BsiWI BsmBI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI EaeI EagI EcoNI EcoRI EcoRV FseI FspI FspAI HpaI KpnI MscI NaeI NgoMIV NheI NotI NsiI PacI PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI SapI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XmaI ZraI