################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT14I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 230 CCGC CCGC(-3/-1) AlwNI 203 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 325 ATTAAT AT^TAAT AsnI 325 ATTAAT AT^TAAT AvaII 170 GGACC G^G[AT]CC BbvI 985 GCAGC GCAGC(8/12) BciVI 1177 GTATCC GTATCC(6/5) BcnI 948 CCCGG CC^[GC]GG BglII 545 AGATCT A^GATCT BsaBI 432 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 10 GAGAC GTCTC(1/5) BsmFI 969 GTCCC GGGAC(10/14) BspHI 149 TCATGA T^CATGA BssSI 47 CACGAG CACGAG(-5/-1) BstAPI 914 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 794 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 169 AGGACCC [GA]G^G[ACGT]CC[CT] DrdI 583 GACTTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 227 TGGCCG [CT]^GGCC[GA] EcoO109I 169 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 329 ATGCAT ATGCA^T HapII 949 CCGG C^CGG HgaI 1064 GCGTC GACGC(5/10) HincII 862 GTCAAC GT[CT]^[GA]AC HindII 862 GTCAAC GT[CT]^[GA]AC HpaII 949 CCGG C^CGG MamI 432 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 949 CCGG C^CGG NciI 948 CCCGG CC^[GC]GG NsiI 329 ATGCAT ATGCA^T PflMI 312 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 169 AGGACCC [GA]G^G[AT]CC[CT] PshBI 325 ATTAAT AT^TAAT RcaI 149 TCATGA T^CATGA SspI 499 AATATT AAT^ATT StuI 234 AGGCCT AGG^CCT TseI 985 GCAGC G^C[AT]GC Van91I 312 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 917 GAATTT [GA]^AATT[CT] 976 AAATTC AfaI 94 GTAC GT^AC 369 GTAC ApoI 917 GAATTT [GA]^AATT[CT] 976 AAATTC BfaI 644 CTAG C^TAG 853 CTAG BsaJI 111 CCTGGG C^C[ACGT][ACGT]GG 269 CCTGGG BsgI 408 CTGCAC GTGCAG(16/14) 983 GTGCAG BstEII 78 GGTCACC G^GT[ACGT]ACC 789 GGTCACC BstPI 78 GGTCACC G^GT[ACGT]ACC 789 GGTCACC Cfr13I 170 GGACC G^G[ACGT]CC 1050 GGCCC EcoO65I 78 GGTCACC G^GT[ACGT]ACC 789 GGTCACC EcoRV 528 GATATC GAT^ATC 1105 GATATC Fnu4HI 229 GCCGC GC^[ACGT]GC 985 GCAGC ItaI 229 GCCGC GC^[ACGT]GC 985 GCAGC MaeI 644 CTAG C^TAG 853 CTAG MboII 482 TCTTC GAAGA(8/7) 775 TCTTC MseI 326 TTAA T^TAA 658 TTAA MslI 792 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1137 CACAAAAGTG NlaIV 170 GGACCC GG[ACGT]^[ACGT]CC 265 GGTTCC PleI 849 GACTC GAGTC(4/5) 1075 GAGTC RsaI 94 GTAC GT^AC 369 GTAC Sau96I 170 GGACC G^G[ACGT]CC 1050 GGCCC SexAI 71 ACCAGGT A^CC[AT]GGT 188 ACCTGGT SfcI 843 CTACAG C^T[GA][CT]AG 1041 CTACAG Tru9I 326 TTAA T^TAA 658 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI AlwI BpmI BsiYI BslI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII FokI HaeIII HinfI HphI MaeIII MboI MflI MnlI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI TfiI Tsp45I Tsp509I TspRI XhoII