(Circular) (Six-base) MAPSORT of: pFN21AE9568.seq Check: 1801 from: 1 to: 5899 pFN21AE9568 5899 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3173 3173 Size: 5899 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3709 278 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3242 1937 Size: 1305 4594 Acc65I G'GTAC_C Cuts at: 3220 3220 Size: 5899 AclI AA'CG_TT Cuts at: 1563 4026 4399 1563 Size: 2463 373 3063 Fragments arranged by size: 3063 2463 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2661 2980 3962 5346 5614 1632 Size: 398 631 319 982 1384 268 1917 Fragments arranged by size: 1917 1384 982 631 398 319 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5702 AflIII A'CryG_T Cuts at: 1203 4798 5775 1203 Size: 3595 977 1327 Fragments arranged by size: 3595 1327 977 AgeI A'CCGG_T Cuts at: 4730 4730 Size: 5899 AhdI GACnn_n'nnGTC Cuts at: 4628 4628 Size: 5899 AleI CACnn'nnGTG Cuts at: 1166 3146 1166 Size: 1980 3919 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5899 AlwNI CAG_nnn'CTG Cuts at: 2213 3642 5214 2213 Size: 1429 1572 2898 Fragments arranged by size: 2898 1572 1429 ApaLI G'TGCA_C Cuts at: 3956 5112 3956 Size: 1156 4743 ApoI r'AATT_y Cuts at: 1493 2922 2981 3208 3434 3586 5695 1493 Size: 1429 59 227 226 152 2109 1697 Fragments arranged by size: 2109 1697 1429 227 226 152 59 AseI AT'TA_AT Cuts at: 160 1032 2331 4453 160 Size: 872 1299 2122 1606 Fragments arranged by size: 2122 1606 1299 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 AvaI C'yCGr_G Cuts at: 1870 1943 3224 1870 Size: 73 1281 4545 Fragments arranged by size: 4545 1281 73 BamHI G'GATC_C Cuts at: 3229 3596 3229 Size: 367 5532 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3220 4675 618 Size: 325 217 627 1433 1455 1842 Fragments arranged by size: 1842 1455 1433 627 325 217 BanII G_rGCy'C Cuts at: 1391 3218 1391 Size: 1827 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4090 4124 1903 Size: 34 2153 34 3678 Fragments arranged by size: 3678 2153 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3193 3791 5001 3193 Size: 598 1210 4091 Fragments arranged by size: 4091 1210 598 BclI T'GATC_A Cuts at: 1548 1548 Size: 5899 BfrBI ATG'CAT Cuts at: 2336 2336 Size: 5899 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4510 136 Size: 107 122 71 631 605 2838 1525 Fragments arranged by size: 2838 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2550 5894 2550 Size: 3344 2555 BlpI GC'TnA_GC Cuts at: 3316 3316 Size: 5899 Bme1580I G_kGCm'C Cuts at: 1790 3960 5116 1790 Size: 2170 1156 2573 Fragments arranged by size: 2573 2170 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5899 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2398 3630 4588 Size: 416 53 83 399 24 981 1232 958 Cuts at: 4588 442 Size: 1753 Fragments arranged by size: 1753 1232 981 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5899 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2539 2866 4559 1239 Size: 1061 239 327 1693 2579 Fragments arranged by size: 2579 1693 1061 327 239 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4024 4889 5187 4024 Size: 865 298 4736 Fragments arranged by size: 4736 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4562 882 Size: 617 3063 2219 Fragments arranged by size: 3063 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3690 493 Size: 711 2486 2702 Fragments arranged by size: 2702 2486 711 BsaBI GATnn'nnATC Cuts at: 2441 5893 2441 Size: 3452 2447 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3706 4088 4723 275 Size: 53 83 186 936 2173 382 635 1451 Fragments arranged by size: 2173 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4332 4730 5004 5151 5562 4332 Size: 398 274 147 411 4669 Fragments arranged by size: 4669 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 1178 Size: 30 165 30 1134 30 4510 Fragments arranged by size: 4510 1134 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5899 BseYI C'CCAG_C Cuts at: 5102 5598 5102 Size: 496 5403 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2398 3009 1163 Size: 573 662 611 4053 Fragments arranged by size: 4053 662 611 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3349 4110 5138 1540 Size: 275 186 1348 761 1028 2301 Fragments arranged by size: 2301 1348 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3960 4045 5116 3960 Size: 85 1071 4743 Fragments arranged by size: 4743 1071 85 BsmI GAATG_Cn' Cuts at: 3410 3503 3410 Size: 93 5806 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3218 3960 4045 5116 1391 Size: 399 1428 742 85 1071 2174 Fragments arranged by size: 2174 1428 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5562 5562 Size: 5899 BspHI T'CATG_A Cuts at: 2154 3788 5518 2154 Size: 1634 1730 2535 Fragments arranged by size: 2535 1730 1634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2782 3046 3173 3254 844 Size: 1938 264 127 81 3489 Fragments arranged by size: 3489 1938 264 127 81 BsrBI CCG'CTC Cuts at: 1394 1823 3786 1394 Size: 429 1963 3507 Fragments arranged by size: 3507 1963 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4394 4568 65 Size: 1183 3146 174 1396 Fragments arranged by size: 3146 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4543 4730 1700 Size: 110 2733 187 2869 Fragments arranged by size: 2869 2733 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5899 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5899 BssSI C'ACGA_G Cuts at: 2052 3959 4971 2052 Size: 1907 1012 2980 Fragments arranged by size: 2980 1907 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2925 794 Size: 2131 3768 BstBI TT'CG_AA Cuts at: 4703 4703 Size: 5899 BstEII G'GTnAC_C Cuts at: 1184 2083 2794 1184 Size: 899 711 4289 Fragments arranged by size: 4289 899 711 BstXI CCAn_nnnn'nTGG Cuts at: 2806 2806 Size: 5899 BstYI r'GATC_y Cuts at: 1972 2497 2550 2680 3229 3596 3981 3998 5450 Size: 525 53 130 549 367 385 17 1452 Cuts at: 5450 5536 5548 5894 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 549 525 385 367 346 130 86 53 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4408 Fragments arranged by size: 4408 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5160 Fragments arranged by size: 5160 683 56 BtsI GCAGTG_nn' Cuts at: 2064 2817 3415 4209 4229 2064 Size: 753 598 794 20 3734 Fragments arranged by size: 3734 794 753 598 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5899 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5864 DraI TTT'AAA Cuts at: 3203 3203 Size: 5899 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2594 4906 809 Size: 507 1278 2312 1802 Fragments arranged by size: 2312 1802 1278 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 3346 4235 Size: 54 1425 50 275 18 402 1114 889 Cuts at: 4235 5864 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1114 889 402 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3346 1537 Size: 275 1534 4090 Fragments arranged by size: 4090 1534 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3829 1353 Size: 2476 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4486 4860 5006 387 Size: 1064 138 2897 374 146 1280 Fragments arranged by size: 2897 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2300 2539 2661 2866 2980 3962 Size: 393 398 270 239 122 205 114 982 Cuts at: 3962 4559 5346 5614 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 982 787 597 398 393 270 268 239 205 122 114 EcoO109I rG'GnC_Cy Cuts at: 2175 2175 Size: 5899 EcoRI G'AATT_C Cuts at: 3208 3586 3208 Size: 378 5521 EcoRV GAT'ATC Cuts at: 2535 3112 2535 Size: 577 5322 FspI TGC'GCA Cuts at: 4405 4405 Size: 5899 HaeII r_GCGC'y Cuts at: 1678 5046 1678 Size: 3368 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2950 2982 4540 4572 4614 Size: 32 135 32 1378 32 1558 32 42 Cuts at: 4614 4646 1373 Size: 32 2626 Fragments arranged by size: 2626 1558 1378 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2869 3243 3489 669 Size: 988 281 931 374 246 3079 Fragments arranged by size: 3079 988 931 374 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5899 HpaI GTT'AAC Cuts at: 3489 3489 Size: 5899 KpnI G_GTAC'C Cuts at: 3224 3224 Size: 5899 MfeI C'AATT_G Cuts at: 3498 3498 Size: 5899 MluI A'CGCG_T Cuts at: 5775 5775 Size: 5899 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2272 5013 5197 1305 Size: 967 2741 184 2007 Fragments arranged by size: 2741 2007 967 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4077 Fragments arranged by size: 4077 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2801 3146 3857 4216 4375 518 Size: 648 1635 345 711 359 159 2042 Fragments arranged by size: 2042 1635 711 648 359 345 159 MspA1I CmG'CkG Cuts at: 1606 3315 3992 5140 5385 1606 Size: 1709 677 1148 245 2120 Fragments arranged by size: 2120 1709 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4408 Fragments arranged by size: 4408 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5899 NotI GC'GGCC_GC Cuts at: 3346 3346 Size: 5899 NsiI A_TGCA'T Cuts at: 2338 2338 Size: 5899 NspI r_CATG'y Cuts at: 1132 1229 3257 4802 1132 Size: 97 2028 1545 2229 Fragments arranged by size: 2229 2028 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5899 PciI A'CATG_T Cuts at: 4798 4798 Size: 5899 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 1108 Size: 1215 4684 PfoI T'CCnGG_A Cuts at: 2555 2555 Size: 5899 PmeI GTTT'AAAC Cuts at: 3203 3203 Size: 5899 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3968 4000 5481 5513 3968 Size: 32 1481 32 4354 Fragments arranged by size: 4354 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2175 2175 Size: 5899 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5899 PsiI TTA'TAA Cuts at: 3469 3469 Size: 5899 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2254 2286 2254 Size: 32 5867 PstI C_TGCA'G Cuts at: 830 3251 830 Size: 2421 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5899 SalI G'TCGA_C Cuts at: 1936 3241 1936 Size: 1305 4594 SbfI CC_TGCA'GG Cuts at: 3251 3251 Size: 5899 ScaI AGT'ACT Cuts at: 4147 5859 4147 Size: 1712 4187 SexAI A'CCwGG_T Cuts at: 2076 2193 2076 Size: 117 5782 SfcI C'TryA_G Cuts at: 826 1045 2848 3046 3247 4382 5063 5254 826 Size: 219 1803 198 201 1135 681 191 1471 Fragments arranged by size: 1803 1471 1135 681 219 201 198 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5899 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5899 SmaI CCC'GGG Cuts at: 1872 3226 1872 Size: 1354 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4003 4904 5166 820 Size: 197 926 2060 901 262 1553 Fragments arranged by size: 2060 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3690 493 Size: 3197 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5899 SphI G_CATG'C Cuts at: 1132 3257 1132 Size: 2125 3774 SspI AAT'ATT Cuts at: 5 52 2506 3823 5 Size: 47 2454 1317 2081 Fragments arranged by size: 2454 2081 1317 47 StuI AGG'CCT Cuts at: 1517 2241 1517 Size: 724 5175 StyI C'CwwG_G Cuts at: 513 1065 2004 3338 513 Size: 552 939 1334 3074 Fragments arranged by size: 3074 1334 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3926 3943 4096 4281 4726 325 Size: 1255 2346 17 153 185 445 1498 Fragments arranged by size: 2346 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2098 4145 5857 Size: 274 80 33 51 1443 121 2047 1712 Cuts at: 5857 96 Size: 138 Fragments arranged by size: 2047 1712 1443 274 138 121 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5859 5891 5859 Size: 32 5867 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5899 XbaI T'CTAG_A Cuts at: 3235 3235 Size: 5899 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5899 XmaI C'CCGG_G Cuts at: 1870 3224 1870 Size: 1354 4545 XmnI GAAnn'nnTTC Cuts at: 4028 4028 Size: 5899 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3707 276 Size: 53 83 186 936 2173 2468 Fragments arranged by size: 2468 2173 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI