################################## # NO CUT ENZYMES ( 212 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 261 AAATTT [GA]^AATT[CT] ApoI 261 AAATTT [GA]^AATT[CT] BbsI 146 GAAGAC GAAGAC(2/6) BbvI 70 GCTGC GCAGC(8/12) BglII 221 AGATCT A^GATCT BmyI 194 GGGCAC G[AGT]GC[ACT]^C BpuAI 146 GAAGAC GAAGAC(2/6) Bsp1286I 194 GGGCAC G[AGT]GC[ACT]^C BstBI 50 TTCGAA TT^CGAA BstYI 221 AGATCT [GA]^GATC[CT] Cac8I 12 GCAGGC GC[ACGT]^[ACGT]GC DdeI 155 CTGAG C^T[ACGT]AG DpnII 222 GATC ^GATC EarI 253 GAAGAG CTCTTC(1/4) Fnu4HI 70 GCTGC GC^[ACGT]GC HaeIII 15 GGCC GG^CC HinfI 77 GATTC G^A[ACGT]TC ItaI 70 GCTGC GC^[ACGT]GC Ksp632I 253 GAAGAG CTCTTC(1/4) MboI 222 GATC ^GATC MflI 221 AGATCT [GA]^GATC[CT] NdeII 222 GATC ^GATC NspV 50 TTCGAA TT^CGAA PstI 71 CTGCAG CTGCA^G Sau3AI 222 GATC ^GATC SfcI 71 CTGCAG C^T[GA][CT]AG SfuI 50 TTCGAA TT^CGAA TaqI 51 TCGA T^CGA TfiI 77 GATTC G^A[AT]TC TseI 70 GCTGC G^C[AT]GC Tsp509I 262 AATT ^AATT TthHB8I 51 TCGA T^CGA XhoII 221 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AvaII 201 GGACC G^G[AT]CC 242 GGACC BsrI 87 ACTGG ACTGG(1/-1) 198 ACTGG Cfr13I 201 GGACC G^G[ACGT]CC 242 GGACC MseI 94 TTAA T^TAA 188 TTAA MwoI 16 GCCGTTTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 228 GCCTAAAGAGC Sau96I 201 GGACC G^G[ACGT]CC 242 GGACC SfaNI 25 GCATC GCATC(5/9) 216 GCATC Tru9I 94 TTAA T^TAA 188 TTAA TspRI 197 CACTG CA[GC]TG[ACGT][ACGT]^ 238 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### BstF5I FokI MboII MnlI