################################## # NO CUT ENZYMES ( 179 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 484 GAATTT [GA]^AATT[CT] AfaI 321 GTAC GT^AC ApoI 484 GAATTT [GA]^AATT[CT] AseI 199 ATTAAT AT^TAAT AsnI 199 ATTAAT AT^TAAT AvaII 9 GGACC G^G[AT]CC BanI 106 GGCACC G^G[CT][GA]CC BpmI 552 CTGGAG CTGGAG(16/14) BsiEI 120 CGACCG CG[GA][CT]^CG BsiYI 129 CCAAGCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 129 CCAAGCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 145 GAGAC GTCTC(1/5) BsmBI 145 GAGACG CGTCTC(1/5) BsmFI 440 GGGAC GGGAC(10/14) BsrI 543 CCAGT ACTGG(1/-1) BssKI 12 CCTGG ^CC[ACGT]GG BstF5I 27 GGATG GGATG(2/0) BstNI 12 CCTGG CC^[AT]GG Cac8I 538 GCCTGC GC[ACGT]^[ACGT]GC Cfr13I 9 GGACC G^G[ACGT]CC DraI 169 TTTAAA TTT^AAA DraII 8 AGGACCT [GA]G^G[ACGT]CC[CT] EarI 214 GAAGAG CTCTTC(1/4) EcoO109I 8 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 12 CCTGG ^CC[AT]GG FokI 27 GGATG GGATG(9/13) HaeIII 295 GGCC GG^CC HgaI 147 GACGC GACGC(5/10) HinfI 37 GAGTC G^A[ACGT]TC Ksp632I 214 GAAGAG CTCTTC(1/4) MvaI 12 CCTGG CC^[AT]GG NdeI 533 CATATG CA^TATG PleI 37 GAGTC GAGTC(4/5) PpuMI 8 AGGACCT [GA]G^G[AT]CC[CT] PshBI 199 ATTAAT AT^TAAT RsaI 321 GTAC GT^AC SapI 214 GAAGAGC GCTCTTC(1/4) Sau96I 9 GGACC G^G[ACGT]CC ScrFI 12 CCTGG CC^[ACGT]GG SspI 361 AATATT AAT^ATT StuI 294 AGGCCT AGG^CCT TspRI 544 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AspHI 19 GAGCTC G[AT]GC[AT]^C 333 GAGCTC BbvI 84 GCAGC GCAGC(8/12) 95 GCTGC BsaWI 122 ACCGGA [AT]^CCGG[AT] 315 TCCGGT BsiHKAI 19 GAGCTC G[AT]GC[AT]^C 333 GAGCTC DdeI 64 CTAAG C^T[ACGT]AG 151 CTCAG HapII 123 CCGG C^CGG 316 CCGG HpaII 123 CCGG C^CGG 316 CCGG MnlI 239 CCTC CCTC(7/6) 7 GAGG MspI 123 CCGG C^CGG 316 CCGG NlaIV 106 GGCACC GG[ACGT]^[ACGT]CC 125 GGAACC NspI 376 GCATGT [GA]CATG^[CT] 476 ACATGC SacI 19 GAGCTC GAGCT^C 333 GAGCTC TaqI 35 TCGA T^CGA 80 TCGA TseI 84 GCAGC G^C[AT]GC 95 GCTGC Tsp45I 380 GTGAC ^GT[GC]AC 571 GTGAC TthHB8I 35 TCGA T^CGA 80 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AciI AluI BanII BmyI BsaJI Bsp1286I DpnII EcoT14I Fnu4HI ItaI MaeIII MboI MboII MseI MwoI NdeII NlaIII Sau3AI StyI Tru9I Tsp509I