(Circular) (Six-base) MAPSORT of: pFN21AE6502.seq Check: 3310 from: 1 to: 5326 pFN21AE6502 5326 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3136 278 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2669 1937 Size: 732 4594 Acc65I G'GTAC_C Cuts at: 2647 2647 Size: 5326 AclI AA'CG_TT Cuts at: 1563 3453 3826 1563 Size: 1890 373 3063 Fragments arranged by size: 3063 1890 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 2570 3389 4773 5041 1632 Size: 618 320 819 1384 268 1917 Fragments arranged by size: 1917 1384 819 618 320 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5129 AflIII A'CryG_T Cuts at: 1203 2623 4225 5202 1203 Size: 1420 1602 977 1327 Fragments arranged by size: 1602 1420 1327 977 AgeI A'CCGG_T Cuts at: 4157 4157 Size: 5326 AhdI GACnn_n'nnGTC Cuts at: 4055 4055 Size: 5326 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2560 1184 Size: 1376 3950 AlwNI CAG_nnn'CTG Cuts at: 3069 4641 3069 Size: 1572 3754 ApaLI G'TGCA_C Cuts at: 3383 4539 3383 Size: 1156 4170 ApoI r'AATT_y Cuts at: 1493 2489 2635 2861 3013 5122 1493 Size: 996 146 226 152 2109 1697 Fragments arranged by size: 2109 1697 996 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 2205 3880 160 Size: 872 1173 1675 1606 Fragments arranged by size: 1675 1606 1173 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 AvaI C'yCGr_G Cuts at: 1870 1943 2651 1870 Size: 73 708 4545 Fragments arranged by size: 4545 708 73 BamHI G'GATC_C Cuts at: 2656 3023 2656 Size: 367 4959 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2111 2647 4102 618 Size: 325 217 627 324 536 1455 1842 Fragments arranged by size: 1842 1455 627 536 325 324 217 BanII G_rGCy'C Cuts at: 1391 2028 2226 2342 2645 1391 Size: 637 198 116 303 4072 Fragments arranged by size: 4072 637 303 198 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4355 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3517 3551 1903 Size: 34 1580 34 3678 Fragments arranged by size: 3678 1580 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3218 4428 3218 Size: 1210 4116 BclI T'GATC_A Cuts at: 1548 1548 Size: 5326 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3937 136 Size: 107 122 71 631 605 2265 1525 Fragments arranged by size: 2265 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5321 5321 Size: 5326 BlpI GC'TnA_GC Cuts at: 2743 2743 Size: 5326 Bme1580I G_kGCm'C Cuts at: 1790 3387 4543 1790 Size: 1597 1156 2573 Fragments arranged by size: 2573 1597 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5326 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3057 4015 442 Size: 416 53 83 399 24 1640 958 1753 Fragments arranged by size: 1753 1640 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5326 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2578 3986 1239 Size: 1339 1408 2579 Fragments arranged by size: 2579 1408 1339 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3451 4316 4614 3451 Size: 865 298 4163 Fragments arranged by size: 4163 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3989 882 Size: 617 2490 2219 Fragments arranged by size: 2490 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3117 493 Size: 711 1913 2702 Fragments arranged by size: 2702 1913 711 BsaBI GATnn'nnATC Cuts at: 5320 5320 Size: 5326 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3133 3515 4150 275 Size: 53 83 186 936 1600 382 635 1451 Fragments arranged by size: 1600 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2127 2320 3759 4157 4431 4578 4989 2127 Size: 193 1439 398 274 147 411 2464 Fragments arranged by size: 2464 1439 411 398 274 193 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2368 2398 1178 Size: 30 165 30 965 30 4106 Fragments arranged by size: 4106 965 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5326 BseYI C'CCAG_C Cuts at: 2607 4529 5025 2607 Size: 1922 496 2908 Fragments arranged by size: 2908 1922 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4753 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2128 2776 3537 4565 1540 Size: 275 186 127 648 761 1028 2301 Fragments arranged by size: 2301 1028 761 648 275 186 127 BsiHKAI G_wGCw'C Cuts at: 2028 2342 3387 3472 4543 2028 Size: 314 1045 85 1071 2811 Fragments arranged by size: 2811 1071 1045 314 85 BsmI GAATG_Cn' Cuts at: 2837 2930 2837 Size: 93 5233 BsmBI CGTCTCn'nnnn_ Cuts at: 2144 2144 Size: 5326 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2226 2342 2645 3387 3472 4543 Size: 399 238 198 116 303 742 85 1071 Cuts at: 4543 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 303 238 198 116 85 BspEI T'CCGG_A Cuts at: 4989 4989 Size: 5326 BspHI T'CATG_A Cuts at: 3215 4945 3215 Size: 1730 3596 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2681 844 Size: 1837 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3213 1394 Size: 429 1390 3507 Fragments arranged by size: 3507 1390 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3821 3995 65 Size: 1183 2573 174 1396 Fragments arranged by size: 2573 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3970 4157 1700 Size: 110 2160 187 2869 Fragments arranged by size: 2869 2160 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5326 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5326 BssSI C'ACGA_G Cuts at: 3386 4398 3386 Size: 1012 4314 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5326 BstBI TT'CG_AA Cuts at: 4130 4130 Size: 5326 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5326 BstYI r'GATC_y Cuts at: 1972 2656 3023 3408 3425 4877 4963 4975 5321 Size: 684 367 385 17 1452 86 12 346 Cuts at: 5321 1972 Size: 1977 Fragments arranged by size: 1977 1452 684 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3835 Fragments arranged by size: 3835 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4587 Fragments arranged by size: 4587 683 56 BtsI GCAGTG_nn' Cuts at: 2842 3636 3656 2842 Size: 794 20 4512 Fragments arranged by size: 4512 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5326 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5291 DraI TTT'AAA Cuts at: 2176 2630 2176 Size: 454 4872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4333 809 Size: 507 3017 1802 Fragments arranged by size: 3017 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2773 3662 5291 Size: 54 1425 50 275 18 943 889 1629 Cuts at: 5291 8 Size: 43 Fragments arranged by size: 1629 1425 943 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2773 1537 Size: 275 961 4090 Fragments arranged by size: 4090 961 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2214 3256 1353 Size: 861 1042 3423 Fragments arranged by size: 3423 1042 861 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 3913 4287 4433 387 Size: 1064 138 435 1889 374 146 1280 Fragments arranged by size: 1889 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2026 2340 2026 Size: 314 5012 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2570 2578 3389 3986 4773 5041 Size: 393 618 320 8 811 597 787 268 Cuts at: 5041 1239 Size: 1524 Fragments arranged by size: 1524 811 787 618 597 393 320 268 8 EcoO109I rG'GnC_Cy Cuts at: 2014 2014 Size: 5326 EcoRI G'AATT_C Cuts at: 2635 3013 2635 Size: 378 4948 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2207 2207 Size: 5326 FspI TGC'GCA Cuts at: 3832 3832 Size: 5326 HaeII r_GCGC'y Cuts at: 1678 4473 1678 Size: 2795 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2581 2613 3967 3999 4041 Size: 32 135 32 1009 32 1354 32 42 Cuts at: 4041 4073 1373 Size: 32 2626 Fragments arranged by size: 2626 1354 1009 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2670 2916 669 Size: 988 281 732 246 3079 Fragments arranged by size: 3079 988 732 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5326 HpaI GTT'AAC Cuts at: 2916 2916 Size: 5326 KpnI G_GTAC'C Cuts at: 2651 2651 Size: 5326 MfeI C'AATT_G Cuts at: 2925 2925 Size: 5326 MluI A'CGCG_T Cuts at: 5202 5202 Size: 5326 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4440 4624 1305 Size: 3135 184 2007 Fragments arranged by size: 3135 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3504 Fragments arranged by size: 3504 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3284 3643 3802 518 Size: 648 2118 359 159 2042 Fragments arranged by size: 2118 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2742 3419 4567 4812 1606 Size: 1136 677 1148 245 2120 Fragments arranged by size: 2120 1148 1136 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3835 Fragments arranged by size: 3835 939 552 NdeI CA'TA_TG Cuts at: 387 2539 387 Size: 2152 3174 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5326 NotI GC'GGCC_GC Cuts at: 2773 2773 Size: 5326 NspI r_CATG'y Cuts at: 1132 1229 2385 2485 2627 2684 4229 1132 Size: 97 1156 100 142 57 1545 2229 Fragments arranged by size: 2229 1545 1156 142 100 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5326 PciI A'CATG_T Cuts at: 2623 4225 2623 Size: 1602 3724 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5326 PmeI GTTT'AAAC Cuts at: 2630 2630 Size: 5326 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3395 3427 4908 4940 3395 Size: 32 1481 32 3781 Fragments arranged by size: 3781 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2014 2014 Size: 5326 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5326 PsiI TTA'TAA Cuts at: 2896 2896 Size: 5326 PstI C_TGCA'G Cuts at: 830 2678 830 Size: 1848 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5326 SacI G_AGCT'C Cuts at: 2028 2342 2028 Size: 314 5012 SalI G'TCGA_C Cuts at: 1936 2668 1936 Size: 732 4594 SapI GCTCTTCn'nnn_ Cuts at: 2214 2214 Size: 5326 SbfI CC_TGCA'GG Cuts at: 2678 2678 Size: 5326 ScaI AGT'ACT Cuts at: 3574 5286 3574 Size: 1712 3614 SfcI C'TryA_G Cuts at: 826 1045 2674 3809 4490 4681 826 Size: 219 1629 1135 681 191 1471 Fragments arranged by size: 1629 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5326 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5326 SmaI CCC'GGG Cuts at: 1872 2653 1872 Size: 781 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3430 4331 4593 820 Size: 197 926 1487 901 262 1553 Fragments arranged by size: 1553 1487 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3117 493 Size: 2624 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5326 SphI G_CATG'C Cuts at: 1132 2684 1132 Size: 1552 3774 SspI AAT'ATT Cuts at: 5 52 2368 3250 5 Size: 47 2316 882 2081 Fragments arranged by size: 2316 2081 882 47 StuI AGG'CCT Cuts at: 1517 2301 1517 Size: 784 4542 StyI C'CwwG_G Cuts at: 513 1065 2004 2139 2302 2329 2765 513 Size: 552 939 135 163 27 436 3074 Fragments arranged by size: 3074 939 552 436 163 135 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 3353 3370 3523 3708 4153 325 Size: 1255 549 1224 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1224 549 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3572 5284 96 Size: 274 80 33 51 1443 1595 1712 138 Fragments arranged by size: 1712 1595 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2368 2400 2438 2470 5286 5318 2368 Size: 32 38 32 2816 32 2376 Fragments arranged by size: 2816 2376 38 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5326 XbaI T'CTAG_A Cuts at: 2662 2662 Size: 5326 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5326 XmaI C'CCGG_G Cuts at: 1870 2651 1870 Size: 781 4545 XmnI GAAnn'nnTTC Cuts at: 3455 3455 Size: 5326 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3134 276 Size: 53 83 186 936 1600 2468 Fragments arranged by size: 2468 1600 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI