(Circular) (Six-base) MAPSORT of: pFN21AE5736.seq Check: 3261 from: 1 to: 7201 pFN21AE5736 7201 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5011 278 Size: 53 83 186 936 3475 2468 Fragments arranged by size: 3475 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4190 4375 4544 1937 Size: 2253 185 169 4594 Fragments arranged by size: 4594 2253 185 169 Acc65I G'GTAC_C Cuts at: 4522 4522 Size: 7201 AclI AA'CG_TT Cuts at: 1563 3318 5328 5701 1563 Size: 1755 2010 373 3063 Fragments arranged by size: 3063 2010 1755 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1997 2659 3405 4500 5264 6648 6916 1632 Size: 365 662 746 1095 764 1384 268 1917 Fragments arranged by size: 1917 1384 1095 764 746 662 365 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7004 AflIII A'CryG_T Cuts at: 1203 3020 3709 6100 7077 1203 Size: 1817 689 2391 977 1327 Fragments arranged by size: 2391 1817 1327 977 689 AgeI A'CCGG_T Cuts at: 6032 6032 Size: 7201 AhdI GACnn_n'nnGTC Cuts at: 5930 5930 Size: 7201 AleI CACnn'nnGTG Cuts at: 1166 2690 2786 3755 1166 Size: 1524 96 969 4612 Fragments arranged by size: 4612 1524 969 96 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7201 AlwNI CAG_nnn'CTG Cuts at: 3578 4944 6516 3578 Size: 1366 1572 4263 Fragments arranged by size: 4263 1572 1366 ApaLI G'TGCA_C Cuts at: 2808 4047 5258 6414 2808 Size: 1239 1211 1156 3595 Fragments arranged by size: 3595 1239 1211 1156 ApoI r'AATT_y Cuts at: 1493 2668 2979 3462 4510 4736 4888 6997 1493 Size: 1175 311 483 1048 226 152 2109 1697 Fragments arranged by size: 2109 1697 1175 1048 483 311 226 152 AseI AT'TA_AT Cuts at: 160 1032 3003 5755 160 Size: 872 1971 2752 1606 Fragments arranged by size: 2752 1971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7201 AvaI C'yCGr_G Cuts at: 1870 1943 2580 4526 1870 Size: 73 637 1946 4545 Fragments arranged by size: 4545 1946 637 73 BamHI G'GATC_C Cuts at: 3778 4531 4898 3778 Size: 753 367 6081 Fragments arranged by size: 6081 753 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4522 5977 618 Size: 325 217 627 2735 1455 1842 Fragments arranged by size: 2735 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3950 4520 1391 Size: 2559 570 4072 Fragments arranged by size: 4072 2559 570 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2030 3393 3930 928 Size: 971 131 1363 537 4199 Fragments arranged by size: 4199 1363 971 537 131 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2402 2436 4079 4113 5392 5426 1903 Size: 34 465 34 1643 34 1279 34 3678 Fragments arranged by size: 3678 1643 1279 465 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4341 5093 6303 4341 Size: 752 1210 5239 Fragments arranged by size: 5239 1210 752 BclI T'GATC_A Cuts at: 1548 2523 3334 4084 1548 Size: 975 811 750 4665 Fragments arranged by size: 4665 975 811 750 BfrBI ATG'CAT Cuts at: 2918 2918 Size: 7201 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5812 136 Size: 107 122 71 631 605 4140 1525 Fragments arranged by size: 4140 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2270 3858 7196 2270 Size: 1588 3338 2275 Fragments arranged by size: 3338 2275 1588 BlpI GC'TnA_GC Cuts at: 4618 4618 Size: 7201 Bme1580I G_kGCm'C Cuts at: 1790 2812 4051 5262 6418 1790 Size: 1022 1239 1211 1156 2573 Fragments arranged by size: 2573 1239 1211 1156 1022 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7201 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2202 3077 3459 Size: 416 53 83 399 24 785 875 382 Cuts at: 3459 4159 4353 4932 5890 442 Size: 700 194 579 958 1753 Fragments arranged by size: 1753 958 875 785 700 579 416 399 382 194 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4007 1056 Size: 2951 4250 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2369 5861 1239 Size: 1031 99 3492 2579 Fragments arranged by size: 3492 2579 1031 99 Bpu10I CC'TnA_GC Cuts at: 3612 3612 Size: 7201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3964 4455 5326 6191 6489 3964 Size: 491 871 865 298 4676 Fragments arranged by size: 4676 871 865 491 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5864 882 Size: 617 4365 2219 Fragments arranged by size: 4365 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2884 3712 3847 4992 493 Size: 711 1680 828 135 1145 2702 Fragments arranged by size: 2702 1680 1145 828 711 135 BsaBI GATnn'nnATC Cuts at: 2507 3344 4083 4238 7195 2507 Size: 837 739 155 2957 2513 Fragments arranged by size: 2957 2513 837 739 155 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3499 5008 5390 6025 Size: 53 83 186 936 1966 1509 382 635 Cuts at: 6025 275 Size: 1451 Fragments arranged by size: 1966 1509 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3533 5634 6032 6306 6453 6864 3533 Size: 2101 398 274 147 411 3870 Fragments arranged by size: 3870 2101 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4205 4235 1178 Size: 30 165 30 2802 30 4144 Fragments arranged by size: 4144 2802 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3240 4226 1186 Size: 2054 986 4161 Fragments arranged by size: 4161 2054 986 BseYI C'CCAG_C Cuts at: 6404 6900 6404 Size: 496 6705 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3078 1163 Size: 573 1342 5286 Fragments arranged by size: 5286 1342 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4651 5412 6440 1540 Size: 275 186 2650 761 1028 2301 Fragments arranged by size: 2650 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2812 3950 4051 4471 5262 5347 6418 2812 Size: 1138 101 420 791 85 1071 3595 Fragments arranged by size: 3595 1138 1071 791 420 101 85 BsmI GAATG_Cn' Cuts at: 4712 4805 4712 Size: 93 7108 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2812 3950 4051 4471 4520 5262 5347 Size: 399 1022 1138 101 420 49 742 85 Cuts at: 5347 6418 1391 Size: 1071 2174 Fragments arranged by size: 2174 1138 1071 1022 742 420 399 101 85 49 BspEI T'CCGG_A Cuts at: 6864 6864 Size: 7201 BspHI T'CATG_A Cuts at: 5090 6820 5090 Size: 1730 5471 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4322 4556 844 Size: 3478 234 3489 Fragments arranged by size: 3489 3478 234 BsrBI CCG'CTC Cuts at: 1394 1823 5088 1394 Size: 429 3265 3507 Fragments arranged by size: 3507 3265 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3769 5696 5870 65 Size: 1183 2521 1927 174 1396 Fragments arranged by size: 2521 1927 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5845 6032 1700 Size: 110 4035 187 2869 Fragments arranged by size: 4035 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7201 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7201 BssSI C'ACGA_G Cuts at: 5261 6273 5261 Size: 1012 6189 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7201 BstBI TT'CG_AA Cuts at: 6005 6005 Size: 7201 BstEII G'GTnAC_C Cuts at: 1184 4334 1184 Size: 3150 4051 BstXI CCAn_nnnn'nTGG Cuts at: 2461 2692 3851 4393 2461 Size: 231 1159 542 5269 Fragments arranged by size: 5269 1159 542 231 BstYI r'GATC_y Cuts at: 1972 2270 2588 3339 3517 3778 3858 4531 4898 Size: 298 318 751 178 261 80 673 367 Cuts at: 4898 5283 5300 6752 6838 6850 7196 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 751 673 385 367 346 318 298 261 178 86 80 17 12 BstZ17I GTA'TAC Cuts at: 4376 4376 Size: 7201 BtgI C'CryG_G Cuts at: 513 1065 3171 513 Size: 552 2106 4543 Fragments arranged by size: 4543 2106 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6462 Fragments arranged by size: 6462 683 56 BtsI GCAGTG_nn' Cuts at: 4135 4717 5511 5531 4135 Size: 582 794 20 5805 Fragments arranged by size: 5805 794 582 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7201 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2718 2753 529 Size: 35 2154 35 4977 Fragments arranged by size: 4977 2154 35 35 DraI TTT'AAA Cuts at: 4505 4505 Size: 7201 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6208 809 Size: 507 4892 1802 Fragments arranged by size: 4892 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2068 2472 2485 Size: 54 1425 50 275 18 238 404 13 Cuts at: 2485 3918 4256 4325 4648 5537 7166 8 Size: 1433 338 69 323 889 1629 43 Fragments arranged by size: 1629 1433 1425 889 404 338 323 275 238 69 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 4648 1537 Size: 275 2836 4090 Fragments arranged by size: 4090 2836 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3188 5131 1353 Size: 1835 1943 3423 Fragments arranged by size: 3423 1943 1835 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5788 6162 6308 387 Size: 1064 138 4199 374 146 1280 Fragments arranged by size: 4199 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3948 3948 Size: 7201 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1997 2270 2369 2659 3405 4500 5264 Size: 393 365 273 99 290 746 1095 764 Cuts at: 5264 5861 6648 6916 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1095 787 764 746 597 393 365 290 273 268 99 EcoNI CCTnn'n_nnAGG Cuts at: 2257 2257 Size: 7201 EcoO109I rG'GnC_Cy Cuts at: 3084 4103 3084 Size: 1019 6182 EcoRI G'AATT_C Cuts at: 2668 4510 4888 2668 Size: 1842 378 4981 Fragments arranged by size: 4981 1842 378 EcoRV GAT'ATC Cuts at: 2698 3328 2698 Size: 630 6571 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2455 2455 Size: 7201 FspI TGC'GCA Cuts at: 5707 5707 Size: 7201 HaeII r_GCGC'y Cuts at: 1678 6348 1678 Size: 4670 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2672 2704 5842 5874 5916 Size: 32 135 32 1100 32 3138 32 42 Cuts at: 5916 5948 1373 Size: 32 2626 Fragments arranged by size: 3138 2626 1100 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2827 3511 4545 4791 669 Size: 988 281 889 684 1034 246 3079 Fragments arranged by size: 3079 1034 988 889 684 281 246 HindIII A'AGCT_T Cuts at: 748 4135 748 Size: 3387 3814 HpaI GTT'AAC Cuts at: 4791 4791 Size: 7201 KpnI G_GTAC'C Cuts at: 4526 4526 Size: 7201 MfeI C'AATT_G Cuts at: 2859 4800 2859 Size: 1941 5260 MluI A'CGCG_T Cuts at: 7077 7077 Size: 7201 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3609 6315 6499 1305 Size: 2304 2706 184 2007 Fragments arranged by size: 2706 2304 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2070 2487 3920 10 Size: 54 1425 343 238 417 1433 3291 Fragments arranged by size: 3291 1433 1425 417 343 238 54 MslI CAynn'nnrTG Cuts at: 518 1166 2213 2348 2690 2786 3019 3755 5159 Size: 648 1047 135 342 96 233 736 1404 Cuts at: 5159 5518 5677 518 Size: 359 159 2042 Fragments arranged by size: 2042 1404 1047 736 648 359 342 233 159 135 96 MspA1I CmG'CkG Cuts at: 1606 2974 3578 4617 5294 6442 6687 1606 Size: 1368 604 1039 677 1148 245 2120 Fragments arranged by size: 2120 1368 1148 1039 677 604 245 NcoI C'CATG_G Cuts at: 513 1065 3171 513 Size: 552 2106 4543 Fragments arranged by size: 4543 2106 552 NdeI CA'TA_TG Cuts at: 387 2920 387 Size: 2533 4668 NheI G'CTAG_C Cuts at: 1052 4003 1052 Size: 2951 4250 NotI GC'GGCC_GC Cuts at: 4648 4648 Size: 7201 NsiI A_TGCA'T Cuts at: 2920 2920 Size: 7201 NspI r_CATG'y Cuts at: 1132 1229 3024 4559 6104 1132 Size: 97 1795 1535 1545 2229 Fragments arranged by size: 2229 1795 1545 1535 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7201 PciI A'CATG_T Cuts at: 3020 6100 3020 Size: 3080 4121 PflMI CCAn_nnn'nTGG Cuts at: 1108 3449 1108 Size: 2341 4860 PfoI T'CCnGG_A Cuts at: 3051 3051 Size: 7201 PmeI GTTT'AAAC Cuts at: 4505 4505 Size: 7201 PmlI CAC'GTG Cuts at: 3847 3847 Size: 7201 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5270 5302 6783 6815 5270 Size: 32 1481 32 5656 Fragments arranged by size: 5656 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3084 4103 3084 Size: 1019 6182 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7201 PsiI TTA'TAA Cuts at: 4771 4771 Size: 7201 PstI C_TGCA'G Cuts at: 830 4301 4553 830 Size: 3471 252 3478 Fragments arranged by size: 3478 3471 252 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7201 PvuII CAG'CTG Cuts at: 3578 3578 Size: 7201 SacI G_AGCT'C Cuts at: 3950 3950 Size: 7201 SalI G'TCGA_C Cuts at: 1936 4543 1936 Size: 2607 4594 SanDI GG'GwC_CC Cuts at: 3084 4103 3084 Size: 1019 6182 SbfI CC_TGCA'GG Cuts at: 4553 4553 Size: 7201 ScaI AGT'ACT Cuts at: 5449 7161 5449 Size: 1712 5489 SfcI C'TryA_G Cuts at: 826 1045 3139 3744 4297 4549 5684 6365 6556 Size: 219 2094 605 553 252 1135 681 191 Cuts at: 6556 826 Size: 1471 Fragments arranged by size: 2094 1471 1135 681 605 553 252 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7201 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7201 SmaI CCC'GGG Cuts at: 1872 4528 1872 Size: 2656 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3943 4470 5305 6206 6468 820 Size: 197 926 2000 527 835 901 262 1553 Fragments arranged by size: 2000 1553 926 901 835 527 262 197 SnaBI TAC'GTA Cuts at: 493 4992 493 Size: 4499 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7201 SphI G_CATG'C Cuts at: 1132 4559 1132 Size: 3427 3774 SspI AAT'ATT Cuts at: 5 52 2716 2816 5125 5 Size: 47 2664 100 2309 2081 Fragments arranged by size: 2664 2309 2081 100 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7201 StyI C'CwwG_G Cuts at: 513 1065 2253 2895 3171 3774 4008 4263 4640 Size: 552 1188 642 276 603 234 255 377 Cuts at: 4640 513 Size: 3074 Fragments arranged by size: 3074 1188 642 603 552 377 276 255 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2916 5228 5245 5398 5583 6028 325 Size: 1255 1336 2312 17 153 185 445 1498 Fragments arranged by size: 2312 1498 1336 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2538 3255 3636 Size: 274 80 33 51 1443 561 717 381 Cuts at: 3636 5447 7159 96 Size: 1811 1712 138 Fragments arranged by size: 1811 1712 1443 717 561 381 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2071 2103 2304 2336 7161 7193 2071 Size: 32 201 32 4825 32 2079 Fragments arranged by size: 4825 2079 201 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7201 XbaI T'CTAG_A Cuts at: 4537 4537 Size: 7201 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3169 3200 4360 3169 Size: 31 1160 6010 Fragments arranged by size: 6010 1160 31 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7201 XmaI C'CCGG_G Cuts at: 1870 4526 1870 Size: 2656 4545 XmnI GAAnn'nnTTC Cuts at: 2672 5330 2672 Size: 2658 4543 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5009 276 Size: 53 83 186 936 3475 2468 Fragments arranged by size: 3475 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI Bsu36I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI