(Linear) (Six-base) MAPSORT of: KIEE5736.seq Check: 7970 from: 1 to: 2496 KIEE5736.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AccI GT'mk_AC Cuts at: 0 2185 2370 2496 Size: 2185 185 126 Fragments arranged by size: 2185 185 126 AclI AA'CG_TT Cuts at: 0 1313 2496 Size: 1313 1183 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 654 1400 2495 2496 Size: 654 746 1095 1 Fragments arranged by size: 1095 746 654 1 AflIII A'CryG_T Cuts at: 0 1015 1704 2496 Size: 1015 689 792 Fragments arranged by size: 1015 792 689 AleI CACnn'nnGTG Cuts at: 0 685 781 1750 2496 Size: 685 96 969 746 Fragments arranged by size: 969 746 685 96 AlwNI CAG_nnn'CTG Cuts at: 0 1573 2496 Size: 1573 923 ApaLI G'TGCA_C Cuts at: 0 803 2042 2496 Size: 803 1239 454 Fragments arranged by size: 1239 803 454 ApoI r'AATT_y Cuts at: 0 663 974 1457 2496 Size: 663 311 483 1039 Fragments arranged by size: 1039 663 483 311 AseI AT'TA_AT Cuts at: 0 998 2496 Size: 998 1498 AvaI C'yCGr_G Cuts at: 0 575 2496 Size: 575 1921 BamHI G'GATC_C Cuts at: 0 1773 2496 Size: 1773 723 BanII G_rGCy'C Cuts at: 0 1945 2496 Size: 1945 551 BbsI GAAGACnn'nnnn_ Cuts at: 0 25 1388 1925 2496 Size: 25 1363 537 571 Fragments arranged by size: 1363 571 537 25 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 397 431 2074 2108 2496 Size: 397 34 1643 34 388 Fragments arranged by size: 1643 397 388 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2336 2496 Size: 2336 160 BclI T'GATC_A Cuts at: 0 518 1329 2079 2496 Size: 518 811 750 417 Fragments arranged by size: 811 750 518 417 BfrBI ATG'CAT Cuts at: 0 913 2496 Size: 913 1583 BglII A'GATC_T Cuts at: 0 265 1853 2496 Size: 265 1588 643 Fragments arranged by size: 1588 643 265 Bme1580I G_kGCm'C Cuts at: 0 807 2046 2496 Size: 807 1239 450 Fragments arranged by size: 1239 807 450 BmrI ACTGGGnnnn_n' Cuts at: 0 197 1072 1454 2154 2348 2496 Size: 197 875 382 700 194 148 Fragments arranged by size: 875 700 382 197 194 148 BmtI G_CTAG'C Cuts at: 0 2002 2496 Size: 2002 494 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 364 2496 Size: 265 99 2132 Fragments arranged by size: 2132 265 99 Bpu10I CC'TnA_GC Cuts at: 0 1607 2496 Size: 1607 889 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1959 2450 2496 Size: 1959 491 46 Fragments arranged by size: 1959 491 46 BsaAI yAC'GTr Cuts at: 0 879 1707 1842 2496 Size: 879 828 135 654 Fragments arranged by size: 879 828 654 135 BsaBI GATnn'nnATC Cuts at: 0 502 1339 2078 2233 2496 Size: 502 837 739 155 263 Fragments arranged by size: 837 739 502 263 155 BsaHI Gr'CG_yC Cuts at: 0 1494 2496 Size: 1494 1002 BsaWI w'CCGG_w Cuts at: 0 1528 2496 Size: 1528 968 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2200 2230 2496 Size: 2200 30 266 Fragments arranged by size: 2200 266 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1235 2221 2496 Size: 1235 986 275 Fragments arranged by size: 1235 986 275 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1073 2496 Size: 1073 1423 BsiHKAI G_wGCw'C Cuts at: 0 807 1945 2046 2466 2496 Size: 807 1138 101 420 30 Fragments arranged by size: 1138 807 420 101 30 Bsp1286I G_dGCh'C Cuts at: 0 807 1945 2046 2466 2496 Size: 807 1138 101 420 30 Fragments arranged by size: 1138 807 420 101 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2317 2496 Size: 2317 179 BsrDI GCAATG_nn' Cuts at: 0 1764 2496 Size: 1764 732 BstEII G'GTnAC_C Cuts at: 0 2329 2496 Size: 2329 167 BstXI CCAn_nnnn'nTGG Cuts at: 0 456 687 1846 2388 2496 Size: 456 231 1159 542 108 Fragments arranged by size: 1159 542 456 231 108 BstYI r'GATC_y Cuts at: 0 265 583 1334 1512 1773 1853 2496 Size: 265 318 751 178 261 80 643 Fragments arranged by size: 751 643 318 265 261 178 80 BstZ17I GTA'TAC Cuts at: 0 2371 2496 Size: 2371 125 BtgI C'CryG_G Cuts at: 0 1166 2496 Size: 1166 1330 BtsI GCAGTG_nn' Cuts at: 0 2130 2496 Size: 2130 366 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 713 748 2496 Size: 713 35 1748 Fragments arranged by size: 1748 713 35 EaeI y'GGCC_r Cuts at: 0 63 467 480 1913 2251 2320 2496 Size: 63 404 13 1433 338 69 176 Fragments arranged by size: 1433 404 338 176 69 63 13 EarI CTCTTCn'nnn_ Cuts at: 0 1183 2496 Size: 1183 1313 EcoICRI GAG'CTC Cuts at: 0 1943 2496 Size: 1943 553 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 265 364 654 1400 2495 2496 Size: 265 99 290 746 1095 1 Fragments arranged by size: 1095 746 290 265 99 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 252 2496 Size: 252 2244 EcoO109I rG'GnC_Cy Cuts at: 0 1079 2098 2496 Size: 1079 1019 398 Fragments arranged by size: 1079 1019 398 EcoRI G'AATT_C Cuts at: 0 663 2496 Size: 663 1833 EcoRV GAT'ATC Cuts at: 0 693 1323 2496 Size: 693 630 1173 Fragments arranged by size: 1173 693 630 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 450 2496 Size: 450 2046 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 667 699 2496 Size: 667 32 1797 Fragments arranged by size: 1797 667 32 HincII GTy'rAC Cuts at: 0 822 1506 2496 Size: 822 684 990 Fragments arranged by size: 990 822 684 HindIII A'AGCT_T Cuts at: 0 2130 2496 Size: 2130 366 MfeI C'AATT_G Cuts at: 0 854 2496 Size: 854 1642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1604 2496 Size: 1604 892 MscI TGG'CCA Cuts at: 0 65 482 1915 2496 Size: 65 417 1433 581 Fragments arranged by size: 1433 581 417 65 MslI CAynn'nnrTG Cuts at: 0 208 343 685 781 1014 1750 2496 Size: 208 135 342 96 233 736 746 Fragments arranged by size: 746 736 342 233 208 135 96 MspA1I CmG'CkG Cuts at: 0 969 1573 2496 Size: 969 604 923 Fragments arranged by size: 969 923 604 NcoI C'CATG_G Cuts at: 0 1166 2496 Size: 1166 1330 NdeI CA'TA_TG Cuts at: 0 915 2496 Size: 915 1581 NheI G'CTAG_C Cuts at: 0 1998 2496 Size: 1998 498 NsiI A_TGCA'T Cuts at: 0 915 2496 Size: 915 1581 NspI r_CATG'y Cuts at: 0 1019 2496 Size: 1019 1477 PciI A'CATG_T Cuts at: 0 1015 2496 Size: 1015 1481 PflMI CCAn_nnn'nTGG Cuts at: 0 1444 2496 Size: 1444 1052 PfoI T'CCnGG_A Cuts at: 0 1046 2496 Size: 1046 1450 PmlI CAC'GTG Cuts at: 0 1842 2496 Size: 1842 654 PpuMI rG'GwC_Cy Cuts at: 0 1079 2098 2496 Size: 1079 1019 398 Fragments arranged by size: 1079 1019 398 PstI C_TGCA'G Cuts at: 0 2296 2496 Size: 2296 200 PvuII CAG'CTG Cuts at: 0 1573 2496 Size: 1573 923 SacI G_AGCT'C Cuts at: 0 1945 2496 Size: 1945 551 SanDI GG'GwC_CC Cuts at: 0 1079 2098 2496 Size: 1079 1019 398 Fragments arranged by size: 1079 1019 398 SfcI C'TryA_G Cuts at: 0 1134 1739 2292 2496 Size: 1134 605 553 204 Fragments arranged by size: 1134 605 553 204 SmlI C'TyrA_G Cuts at: 0 1938 2465 2496 Size: 1938 527 31 Fragments arranged by size: 1938 527 31 SspI AAT'ATT Cuts at: 0 711 811 2496 Size: 711 100 1685 Fragments arranged by size: 1685 711 100 StyI C'CwwG_G Cuts at: 0 248 890 1166 1769 2003 2258 2496 Size: 248 642 276 603 234 255 238 Fragments arranged by size: 642 603 276 255 248 238 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 911 2496 Size: 911 1585 TatI w'GTAC_w Cuts at: 0 533 1250 1631 2496 Size: 533 717 381 865 Fragments arranged by size: 865 717 533 381 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 66 98 299 331 2496 Size: 66 32 201 32 2165 Fragments arranged by size: 2165 201 66 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1164 1195 2355 2496 Size: 1164 31 1160 141 Fragments arranged by size: 1164 1160 141 31 XmnI GAAnn'nnTTC Cuts at: 0 667 2496 Size: 667 1829 Enzymes that do cut: AccI AclI AcuI AflIII AleI AlwNI ApaLI ApoI AseI AvaI BamHI BanII BbsI BcgI BciVI BclI BfrBI BglII Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrDI BstEII BstXI BstYI BstZ17I BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI NspI PciI PflMI PfoI PmlI PpuMI PstI PvuII SacI SanDI SfcI SmlI SspI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI AloI ApaI AscI AsiSI AvrII BaeI BanI BbeI BbvCI BglI BlpI BmgBI BplI BsaI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI FseI FspI FspAI HaeII HpaI KasI KpnI MluI NaeI NarI NgoMIV NotI NruI PacI PasI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI ZraI