################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccI AccIII AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvrII BbrPI BcgI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoT14I EcoT22I FseI HindIII KpnI KspI MamI MluI MroI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SphI SspBI StyI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 1281 CGCG CG^CG AclI 222 AACGTT AA^CGTT AflIII 1003 ACACGT A^C[GA][CT]GT AhdI 533 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 966 CCTCAGG CC^T[ACGT]AGG AspEI 533 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 963 GGACC G^G[AT]CC AviII 595 TGCGCA TGC^GCA BamHI 1175 GGATCC G^GATCC BbeI 265 GGCGCC GGCGC^C BbsI 135 GAAGAC GAAGAC(2/6) BciVI 1315 GGATAC GTATCC(6/5) BglII 1331 AGATCT A^GATCT BpmI 731 CTCCAG CTGGAG(16/14) BpuAI 135 GAAGAC GAAGAC(2/6) BsaAI 1318 TACGTG [CT]AC^GT[GA] BsaI 531 GAGACC GGTCTC(1/5) BsiEI 1466 CGGTCG CG[GA][CT]^CG BsrBI 1414 GAGCGG CCGCTC(-3/-3) BssSI 237 CACGAG CACGAG(-5/-1) BstUI 1281 CGCG CG^CG BstXI 852 CCAGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 966 CCTCAGG CC^T[ACGT]AGG DraII 962 GGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 533 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 966 CCTCAGG CC^T[ACGT]AGG EcoO109I 962 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1291 GAATTC G^AATTC EcoRV 3 GATATC GAT^ATC FauI 1721 GCGGG CCCGC(4/6) FspI 595 TGCGCA TGC^GCA HpaI 225 GTTAAC GTT^AAC KasI 265 GGCGCC G^GCGCC MfeI 482 CAATTG C^AATTG MslI 1645 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 482 CAATTG C^AATTG MvnI 1281 CGCG CG^CG NarI 265 GGCGCC GG^CGCC PpuMI 962 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 222 AACGTT AA^CGTT SbfI 1662 CCTGCAGG CCTGCA^GG SfoI 265 GGCGCC GGC^GCC SmaI 1776 CCCGGG CCC^GGG SmlI 185 CTCAAG C^T[CT][GA]AG SpeI 462 ACTAGT A^CTAGT Sse8387I 1662 CCTGCAGG CCTGCA^GG SspI 77 AATATT AAT^ATT StuI 1303 AGGCCT AGG^CCT TfiI 1528 GATTC G^A[AT]TC XmaCI 1776 CCCGGG C^CCGGG XmaI 1776 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 490 GAATTT [GA]^AATT[CT] 1291 GAATTC AcyI 265 GGCGCC G[GA]^CG[CT]C 1192 GACGCC AlwI 1176 GATCC GGATC(4/5) 1175 GGATC AlwNI 1578 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1657 CAGACCCTG ApoI 490 GAATTT [GA]^AATT[CT] 1291 GAATTC AvaI 424 CCCGAG C^[CT]CG[GA]G 1776 CCCGGG BalI 605 TGGCCA TGG^CCA 914 TGGCCA BanI 265 GGCGCC G^G[CT][GA]CC 355 GGTGCC BbvCI 889 CCTCAGC CCTCAGC(-5/-2) 1345 GCTGAGG BclI 1023 TGATCA T^GATCA 1433 TGATCA BsaHI 265 GGCGCC G[GA]^CG[CT]C 1192 GACGCC BsoBI 424 CCCGAG C^[CT]CG[GA]G 1776 CCCGGG BspMI 1573 ACCTGC ACCTGC(4/8) 1689 ACCTGC BstYI 1175 GGATCC [GA]^GATC[CT] 1331 AGATCT FbaI 1023 TGATCA T^GATCA 1433 TGATCA HaeII 265 GGCGCC [GA]GCGC^[CT] 1370 AGCGCC Hin1I 265 GGCGCC G[GA]^CG[CT]C 1192 GACGCC MflI 1175 GGATCC [GA]^GATC[CT] 1331 AGATCT MluNI 605 TGGCCA TGG^CCA 914 TGGCCA MscI 605 TGGCCA TGG^CCA 914 TGGCCA PflMI 277 CCACCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 346 CCAGCCTGTGG PvuII 337 CAGCTG CAG^CTG 646 CAGCTG SacI 191 GAGCTC GAGCT^C 583 GAGCTC SfaNI 658 GATGC GCATC(5/9) 1284 GCATC TaqI 701 TCGA T^CGA 945 TCGA TthHB8I 701 TCGA T^CGA 945 TCGA Van91I 277 CCACCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 346 CCAGCCTGTGG XcmI 1324 CCAGAGGAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1641 CCATCACATCTGTGG XhoII 1175 GGATCC [GA]^GATC[CT] 1331 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI Asp700I AspHI BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI Tru9I TseI Tsp45I Tsp509I TspRI XmnI