################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaII AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstF5I BstPI BstYI Bsu36I CelII Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoT14I FauI FbaI FokI FseI FspI HaeII HgaI Hin1I HindIII KasI KspI MaeII MamI MflI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI Sau96I SbfI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 77 GGTACC G^GTACC AccI 708 GTATAC GT^[AC][GT]AC AccII 65 CGCG CG^CG AlwNI 130 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 77 GGTACC G^GTACC AvaI 8 CCCGGG C^[CT]CG[GA]G BbsI 664 GAAGAC GAAGAC(2/6) BpuAI 664 GAAGAC GAAGAC(2/6) BsgI 48 GTGCAG GTGCAG(16/14) BsmBI 399 GAGACG CGTCTC(1/5) BsmI 192 GAATGC GAATGC(1/-1) BsoBI 8 CCCGGG C^[CT]CG[GA]G BspMI 50 GCAGGT ACCTGC(4/8) BsrFI 44 GCCGGT [GA]^CCGG[CT] BsrI 288 ACTGG ACTGG(1/-1) Bst1107I 708 GTATAC GTA^TAC BstNI 81 CCTGG CC^[AT]GG BstUI 65 CGCG CG^CG BstXI 479 CCAGACATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 708 GTATAC GTA^TAC Cac8I 168 GCAAGC GC[ACGT]^[ACGT]GC CfoI 64 GCGC GCG^C Cfr10I 44 GCCGGT [GA]^CCGG[CT] DpnII 583 GATC ^GATC EcoNI 691 CCTAATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 81 CCTGG ^CC[AT]GG EcoRV 381 GATATC GAT^ATC EcoT22I 899 ATGCAT ATGCA^T HhaI 64 GCGC GCG^C HinP1I 64 GCGC G^CGC HincII 340 GTTAAC GT[CT]^[GA]AC HindII 340 GTTAAC GT[CT]^[GA]AC HpaI 340 GTTAAC GTT^AAC KpnI 77 GGTACC GGTAC^C MboI 583 GATC ^GATC MfeI 295 CAATTG C^AATTG MslI 898 CATGCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 295 CAATTG C^AATTG MvaI 81 CCTGG CC^[AT]GG MvnI 65 CGCG CG^CG NdeII 583 GATC ^GATC NsiI 899 ATGCAT ATGCA^T NspI 897 ACATGC [GA]CATG^[CT] PflMI 547 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 785 GAGCTC GAGCT^C Sau3AI 583 GATC ^GATC ScaI 731 AGTACT AGT^ACT SexAI 80 ACCTGGT A^CC[AT]GGT SfaNI 144 GCATC GCATC(5/9) SmaI 8 CCCGGG CCC^GGG TspRI 559 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 547 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 8 CCCGGG C^CCGGG XmaI 8 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AciI 638 CCGC CCGC(-3/-1) 97 GCGG AcsI 176 AAATTC [GA]^AATT[CT] 721 GAATTT AfaI 78 GTAC GT^AC 732 GTAC ApoI 176 AAATTC [GA]^AATT[CT] 721 GAATTT Asp700I 664 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 718 GAAGAATTTC AspHI 605 GTGCTC G[AT]GC[AT]^C 785 GAGCTC BalI 245 TGGCCA TGG^CCA 555 TGGCCA BanI 33 GGTGCC G^G[CT][GA]CC 77 GGTACC BanII 4 GAGCCC G[GA]GC[CT]^C 785 GAGCTC BcnI 8 CCCGG CC^[GC]GG 9 CCGGG BpmI 713 CTCCAG CTGGAG(16/14) 289 CTGGAG BseRI 836 CTCCTC GAGGAG(10/8) 219 GAGGAG BsiHKAI 605 GTGCTC G[AT]GC[AT]^C 785 GAGCTC EaeI 245 TGGCCA [CT]^GGCC[GA] 555 TGGCCA HaeIII 246 GGCC GG^CC 556 GGCC HapII 9 CCGG C^CGG 45 CCGG HpaII 9 CCGG C^CGG 45 CCGG MaeIII 931 GTTAC ^GT[ACGT]AC 952 GTTAC MluNI 245 TGGCCA TGG^CCA 555 TGGCCA MscI 245 TGGCCA TGG^CCA 555 TGGCCA MspI 9 CCGG C^CGG 45 CCGG MwoI 135 GCTGAGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 294 GCAATTGAAGC NciI 8 CCCGG CC^[GC]GG 9 CCGGG NdeI 484 CATATG CA^TATG 902 CATATG PvuII 130 CAGCTG CAG^CTG 755 CAGCTG RsaI 78 GTAC GT^AC 732 GTAC SapI 109 GAAGAGC GCTCTTC(1/4) 124 GAAGAGC SspI 510 AATATT AAT^ATT 860 AATATT XmnI 664 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 718 GAAGAATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AluI BbvI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI DdeI EarI Fnu4HI HinfI HphI ItaI Ksp632I MaeI MboII MnlI MseI MspA1I NlaIII NlaIV ScrFI SmlI TfiI Tru9I TseI Tsp509I