Restriction map for
pFN21AE3130
(Linear) (Six-base) MAPSORT of: KIEE3130.seq Check: 8618 from: 1 to: 969
KIEE3130.orf
With 182 enzymes: SgfI *
December 27, 2011 16:33 ..
AccI GT'mk_AC
Cuts at: 0 709 969
Size: 709 260
Acc65I G'GTAC_C
Cuts at: 0 77 969
Size: 77 892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 969
Size: 470 499
AlwNI CAG_nnn'CTG
Cuts at: 0 135 969
Size: 135 834
ApoI r'AATT_y
Cuts at: 0 176 721 969
Size: 176 545 248
Fragments arranged by size:
545 248 176
AvaI C'yCGr_G
Cuts at: 0 8 969
Size: 8 961
BanI G'GyrC_C
Cuts at: 0 33 77 969
Size: 33 44 892
Fragments arranged by size:
892 44 33
BanII G_rGCy'C
Cuts at: 0 8 789 969
Size: 8 781 180
Fragments arranged by size:
781 180 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 671 969
Size: 671 298
BfrBI ATG'CAT
Cuts at: 0 901 969
Size: 901 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 698 969
Size: 310 388 271
Fragments arranged by size:
388 310 271
Bpu10I CC'TnA_GC
Cuts at: 0 909 969
Size: 909 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 645 796 969
Size: 382 263 151 173
Fragments arranged by size:
382 263 173 151
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 234 827 969
Size: 234 593 142
Fragments arranged by size:
593 234 142
BseYI C'CCAG_C
Cuts at: 0 72 969
Size: 72 897
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 969
Size: 69 900
BsiHKAI G_wGCw'C
Cuts at: 0 609 789 969
Size: 609 180 180
Fragments arranged by size:
609 180 180
BsmI GAATG_Cn'
Cuts at: 0 198 969
Size: 198 771
BsmBI CGTCTCn'nnnn_
Cuts at: 0 393 969
Size: 393 576
Bsp1286I G_dGCh'C
Cuts at: 0 8 609 789 969
Size: 8 601 180 180
Fragments arranged by size:
601 180 180 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 41 969
Size: 41 928
BsrFI r'CCGG_y
Cuts at: 0 44 969
Size: 44 925
BstXI CCAn_nnnn'nTGG
Cuts at: 0 486 969
Size: 486 483
BstZ17I GTA'TAC
Cuts at: 0 710 969
Size: 710 259
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 130 969
Size: 130 839
EaeI y'GGCC_r
Cuts at: 0 245 555 969
Size: 245 310 414
Fragments arranged by size:
414 310 245
EarI CTCTTCn'nnn_
Cuts at: 0 104 119 211 969
Size: 104 15 92 758
Fragments arranged by size:
758 104 92 15
EcoICRI GAG'CTC
Cuts at: 0 787 969
Size: 787 182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 470 698 969
Size: 310 160 228 271
Fragments arranged by size:
310 271 228 160
EcoNI CCTnn'n_nnAGG
Cuts at: 0 695 969
Size: 695 274
EcoRV GAT'ATC
Cuts at: 0 383 969
Size: 383 586
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 129 161 969
Size: 129 32 808
Fragments arranged by size:
808 129 32
HincII GTy'rAC
Cuts at: 0 342 969
Size: 342 627
HpaI GTT'AAC
Cuts at: 0 342 969
Size: 342 627
KpnI G_GTAC'C
Cuts at: 0 81 969
Size: 81 888
MfeI C'AATT_G
Cuts at: 0 295 969
Size: 295 674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 969
Size: 233 736
MscI TGG'CCA
Cuts at: 0 247 557 969
Size: 247 310 412
Fragments arranged by size:
412 310 247
MslI CAynn'nnrTG
Cuts at: 0 902 969
Size: 902 67
MspA1I CmG'CkG
Cuts at: 0 132 640 757 969
Size: 132 508 117 212
Fragments arranged by size:
508 212 132 117
NdeI CA'TA_TG
Cuts at: 0 485 903 969
Size: 485 418 66
Fragments arranged by size:
485 418 66
NsiI A_TGCA'T
Cuts at: 0 903 969
Size: 903 66
NspI r_CATG'y
Cuts at: 0 901 969
Size: 901 68
PflMI CCAn_nnn'nTGG
Cuts at: 0 553 969
Size: 553 416
PsiI TTA'TAA
Cuts at: 0 923 969
Size: 923 46
PvuII CAG'CTG
Cuts at: 0 132 757 969
Size: 132 625 212
Fragments arranged by size:
625 212 132
SacI G_AGCT'C
Cuts at: 0 789 969
Size: 789 180
SapI GCTCTTCn'nnn_
Cuts at: 0 104 119 969
Size: 104 15 850
Fragments arranged by size:
850 104 15
ScaI AGT'ACT
Cuts at: 0 733 969
Size: 733 236
SexAI A'CCwGG_T
Cuts at: 0 80 969
Size: 80 889
SmaI CCC'GGG
Cuts at: 0 10 969
Size: 10 959
SmlI C'TyrA_G
Cuts at: 0 361 624 775 969
Size: 361 263 151 194
Fragments arranged by size:
361 263 194 151
SspI AAT'ATT
Cuts at: 0 512 862 969
Size: 512 350 107
Fragments arranged by size:
512 350 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 121 969
Size: 121 848
TatI w'GTAC_w
Cuts at: 0 731 969
Size: 731 238
XmaI C'CCGG_G
Cuts at: 0 8 969
Size: 8 961
XmnI GAAnn'nnTTC
Cuts at: 0 668 722 969
Size: 668 54 247
Fragments arranged by size:
668 247 54
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApoI AvaI BanI BanII
BbsI BfrBI BpmI Bpu10I BpuEI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BstXI BstZ17I
BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoRV Hin4I
HincII HpaI KpnI MfeI MmeI MscI MslI MspA1I
NdeI NsiI NspI PflMI PsiI PvuII SacI SapI
ScaI SexAI SmaI SmlI SspI TaqII TatI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII HindIII KasI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI ZraI