Restriction map for
pFN21AE0947
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbrPI BbvCI
BcgI BfaI BlnI BsaAI BsaBI
BsaI BseAI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MaeII MamI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccII 93 CGCG CG^CG
AcsI 596 AAATTT [GA]^AATT[CT]
AfaI 681 GTAC GT^AC
AflII 1119 CTTAAG C^TTAAG
AflIII 731 ACATGT A^C[GA][CT]GT
AlwNI 937 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 691 CCTGAGG CC^T[ACGT]AGG
Aor51HI 42 AGCGCT AGC^GCT
ApaI 103 GGGCCC GGGCC^C
ApoI 596 AAATTT [GA]^AATT[CT]
AspHI 430 GAGCTC G[AT]GC[AT]^C
BanI 10 GGCGCC G^G[CT][GA]CC
BbeI 10 GGCGCC GGCGC^C
BciVI 134 GGATAC GTATCC(6/5)
BfrI 1119 CTTAAG C^TTAAG
BglI 118 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 374 AGATCT A^GATCT
BlpI 324 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 324 GCTGAGC GC^T[ACGT]AGC
BsaWI 421 ACCGGA [AT]^CCGG[AT]
BsiHKAI 430 GAGCTC G[AT]GC[AT]^C
BsmAI 382 GAGAC GTCTC(1/5)
BspLU11I 731 ACATGT A^CATGT
BsrBI 469 GAGCGG CCGCTC(-3/-3)
BsrDI 447 CATTGC GCAATG(2/0)
BsrFI 79 GCCGGC [GA]^CCGG[CT]
BstAPI 522 GCATGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 93 CGCG CG^CG
BstXI 719 CCAGTGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 691 CCTGAGG CC^T[ACGT]AGG
CelII 324 GCTGAGC GC^T[ACGT]AGC
Cfr10I 79 GCCGGC [GA]^CCGG[CT]
DraIII 662 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 166 GACCCCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 965 CCATGG C^C[GA][CT]GG
EarI 586 GAAGAG CTCTTC(1/4)
Eco47III 42 AGCGCT AGC^GCT
Eco81I 691 CCTGAGG CC^T[ACGT]AGG
KasI 10 GGCGCC G^GCGCC
Ksp632I 586 GAAGAG CTCTTC(1/4)
MspA1I 929 CAGCTG C[AC]G^C[GT]G
MvnI 93 CGCG CG^CG
NaeI 79 GCCGGC GCC^GGC
NarI 10 GGCGCC GG^CGCC
NcoI 965 CCATGG C^CATGG
NgoMIV 79 GCCGGC G^CCGGC
PvuII 929 CAGCTG CAG^CTG
RsaI 681 GTAC GT^AC
SacI 430 GAGCTC GAGCT^C
SfaNI 475 GATGC GCATC(5/9)
SfoI 10 GGCGCC GGC^GCC
SmlI 1119 CTTAAG C^T[CT][GA]AG
SphI 522 GCATGC GCATG^C
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 10 GGCGCC G[GA]^CG[CT]C
82 GGCGTC
BalI 856 TGGCCA TGG^CCA
933 TGGCCA
BamHI 457 GGATCC G^GATCC
485 GGATCC
BbvI 528 GCTGC GCAGC(8/12)
970 GCTGC
BclI 749 TGATCA T^GATCA
959 TGATCA
BpmI 490 CTCCAG CTGGAG(16/14)
994 CTCCAG
BsaHI 10 GGCGCC G[GA]^CG[CT]C
82 GGCGTC
BsgI 1058 CTGCAC GTGCAG(16/14)
722 GTGCAG
BsrI 647 ACTGG ACTGG(1/-1)
719 CCAGT
EaeI 856 TGGCCA [CT]^GGCC[GA]
933 TGGCCA
EcoT14I 853 CCTTGG C^C[AT][AT]GG
965 CCATGG
FbaI 749 TGATCA T^GATCA
959 TGATCA
HaeII 10 GGCGCC [GA]GCGC^[CT]
42 AGCGCT
HgaI 83 GCGTC GACGC(5/10)
329 GCGTC
Hin1I 10 GGCGCC G[GA]^CG[CT]C
82 GGCGTC
MluNI 856 TGGCCA TGG^CCA
933 TGGCCA
MscI 856 TGGCCA TGG^CCA
933 TGGCCA
MseI 601 TTAA T^TAA
1120 TTAA
MslI 402 CATGGTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
577 CATCCAGGTG
NdeI 755 CATATG CA^TATG
1099 CATATG
PflMI 363 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
936 CCAGGCCCTGG
PpuMI 173 GGGTCCT [GA]G^G[AT]CC[CT]
670 GGGACCC
PstI 529 CTGCAG CTGCA^G
971 CTGCAG
SfcI 529 CTGCAG C^T[GA][CT]AG
971 CTGCAG
SmaI 30 CCCGGG CCC^GGG
170 CCCGGG
StyI 853 CCTTGG C^C[AT][AT]GG
965 CCATGG
Tru9I 601 TTAA T^TAA
1120 TTAA
TseI 528 GCTGC G^C[AT]GC
970 GCTGC
Tsp45I 644 GTGAC ^GT[GC]AC
1074 GTCAC
Van91I 363 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
936 CCAGGCCCTGG
XcmI 14 CCAGCAGGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
40 CCAGCGCTGGCTTGG
XmaCI 30 CCCGGG C^CCGGG
170 CCCGGG
XmaI 30 CCCGGG C^CCGGG
170 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AvaI AvaII
BanII BbsI BcnI BmyI BpuAI
BsaJI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI Sau3AI Sau96I
ScrFI TaqI TfiI Tsp509I TspRI
TthHB8I XhoII