(Circular) (Six-base) MAPSORT of: pFN21AE0947.seq Check: 9942 from: 1 to: 5860 pFN21AE0947 5860 bp With 182 enzymes: SgfI * November 18, 2016 17:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3923 5307 5575 1632 Size: 2291 1384 268 1917 Fragments arranged by size: 2291 1917 1384 268 AfeI AGC'GCT Cuts at: 2049 2049 Size: 5860 AflII C'TTAA_G Cuts at: 820 1017 3124 820 Size: 197 2107 3556 Fragments arranged by size: 3556 2107 197 AflIII A'CryG_T Cuts at: 1203 2736 4759 5736 1203 Size: 1533 2023 977 1327 Fragments arranged by size: 2023 1533 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AlwNI CAG_nnn'CTG Cuts at: 2947 3603 5175 2947 Size: 656 1572 3632 Fragments arranged by size: 3632 1572 656 ApaI G_GGCC'C Cuts at: 2112 2112 Size: 5860 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 2601 3169 3395 3547 5656 1493 Size: 1108 568 226 152 2109 1697 Fragments arranged by size: 2109 1697 1108 568 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2035 2111 2175 3185 1870 Size: 73 92 76 64 1010 4545 Fragments arranged by size: 4545 1010 92 76 73 64 BamHI G'GATC_C Cuts at: 2462 2490 3190 3557 2462 Size: 28 700 367 4765 Fragments arranged by size: 4765 700 367 28 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2015 3181 4636 618 Size: 325 217 627 228 1166 1455 1842 Fragments arranged by size: 1842 1455 1166 627 325 228 217 BanII G_rGCy'C Cuts at: 1391 2112 2439 2746 3179 1391 Size: 721 327 307 433 4072 Fragments arranged by size: 4072 721 433 327 307 BbeI G_GCGC'C Cuts at: 2019 2019 Size: 5860 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 2669 2976 3063 928 Size: 971 359 411 307 87 3725 Fragments arranged by size: 3725 971 411 359 307 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4051 4085 1903 Size: 34 2114 34 3678 Fragments arranged by size: 3678 2114 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2133 3752 4962 2133 Size: 1619 1210 3031 Fragments arranged by size: 3031 1619 1210 BclI T'GATC_A Cuts at: 1548 2754 2964 1548 Size: 1206 210 4444 Fragments arranged by size: 4444 1206 210 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2129 4471 136 Size: 107 122 71 631 605 457 2342 1525 Fragments arranged by size: 2342 1525 631 605 457 122 107 71 BglII A'GATC_T Cuts at: 2379 5855 2379 Size: 3476 2384 BlpI GC'TnA_GC Cuts at: 2330 3277 2330 Size: 947 4913 Bme1580I G_kGCm'C Cuts at: 1790 2112 3921 5077 1790 Size: 322 1809 1156 2573 Fragments arranged by size: 2573 1809 1156 322 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3591 4549 442 Size: 416 53 83 399 24 2174 958 1753 Fragments arranged by size: 2174 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2453 3054 2453 Size: 601 5259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2480 2984 4520 1239 Size: 1241 504 1536 2579 Fragments arranged by size: 2579 1536 1241 504 Bpu10I CC'TnA_GC Cuts at: 2457 2708 2457 Size: 251 5609 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2016 2088 3667 4049 Size: 53 83 186 936 483 72 1579 382 Cuts at: 4049 4684 275 Size: 635 1451 Fragments arranged by size: 1579 1451 936 635 483 382 186 83 72 53 BsaWI w'CCGG_w Cuts at: 2426 4293 4691 4965 5112 5523 2426 Size: 1867 398 274 147 411 2763 Fragments arranged by size: 2763 1867 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5635 Fragments arranged by size: 5635 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5860 BseYI C'CCAG_C Cuts at: 5063 5559 5063 Size: 496 5364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2748 3048 1163 Size: 573 1012 300 3975 Fragments arranged by size: 3975 1012 573 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2439 3921 4006 5077 2439 Size: 1482 85 1071 3222 Fragments arranged by size: 3222 1482 1071 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2112 2439 2746 3179 3921 4006 5077 Size: 399 322 327 307 433 742 85 1071 Cuts at: 5077 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 433 399 327 322 307 85 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3215 844 Size: 2371 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2476 3747 1394 Size: 429 653 1271 3507 Fragments arranged by size: 3507 1271 653 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2451 4355 4529 65 Size: 1183 1203 1904 174 1396 Fragments arranged by size: 1904 1396 1203 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2084 4504 4691 1700 Size: 110 274 2420 187 2869 Fragments arranged by size: 2869 2420 274 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 2533 794 Size: 1739 4121 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstXI CCAn_nnnn'nTGG Cuts at: 2731 2731 Size: 5860 BstYI r'GATC_y Cuts at: 1972 2379 2462 2490 3059 3190 3557 3942 3959 Size: 407 83 28 569 131 367 385 17 Cuts at: 3959 5411 5497 5509 5855 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 569 407 385 367 346 131 86 83 28 17 12 Bsu36I CC'TnA_GG Cuts at: 2697 2697 Size: 5860 BtgI C'CryG_G Cuts at: 513 1065 2004 2970 513 Size: 552 939 966 3403 Fragments arranged by size: 3403 966 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2909 487 Size: 683 56 1683 3438 Fragments arranged by size: 3438 1683 683 56 BtsI GCAGTG_nn' Cuts at: 2736 3376 4170 4190 2736 Size: 640 794 20 4406 Fragments arranged by size: 4406 794 640 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DraIII CAC_nnn'GTG Cuts at: 2672 2672 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2177 4867 809 Size: 507 861 2690 1802 Fragments arranged by size: 2690 1802 861 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2861 2938 3307 Size: 54 1425 50 275 18 1031 77 369 Cuts at: 3307 4196 5825 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1031 889 369 275 77 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2586 3790 1353 Size: 1233 1204 3423 Fragments arranged by size: 3423 1233 1204 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2437 2437 Size: 5860 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2480 2984 3923 4520 5307 5575 1239 Size: 393 848 504 939 597 787 268 1524 Fragments arranged by size: 1524 939 848 787 597 504 393 268 EcoO109I rG'GnC_Cy Cuts at: 2102 2179 2676 2944 3033 2102 Size: 77 497 268 89 4929 Fragments arranged by size: 4929 497 268 89 77 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2733 3139 2733 Size: 406 5454 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2019 2051 5007 1678 Size: 341 32 2956 2531 Fragments arranged by size: 2956 2531 341 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2181 2213 2380 2412 4501 Size: 32 135 32 609 32 167 32 2089 Cuts at: 4501 4533 4575 4607 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2089 609 167 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KasI G'GCGC_C Cuts at: 2015 2015 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2071 4974 5158 1305 Size: 766 2903 184 2007 Fragments arranged by size: 2903 2007 766 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2863 2940 10 Size: 54 1425 343 1031 77 2930 Fragments arranged by size: 2930 1425 1031 343 77 54 MslI CAynn'nnrTG Cuts at: 518 1166 2411 2586 3818 4177 4336 518 Size: 648 1245 175 1232 359 159 2042 Fragments arranged by size: 2042 1245 1232 648 359 175 159 MspA1I CmG'CkG Cuts at: 1606 2936 3276 3953 5101 5346 1606 Size: 1330 340 677 1148 245 2120 Fragments arranged by size: 2120 1330 1148 677 340 245 NaeI GCC'GGC Cuts at: 2086 2086 Size: 5860 NarI GG'CG_CC Cuts at: 2016 2016 Size: 5860 NcoI C'CATG_G Cuts at: 513 1065 2004 2970 513 Size: 552 939 966 3403 Fragments arranged by size: 3403 966 939 552 NdeI CA'TA_TG Cuts at: 387 2761 3105 387 Size: 2374 344 3142 Fragments arranged by size: 3142 2374 344 NgoMIV G'CCGG_C Cuts at: 2084 2084 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2531 2740 2932 3218 4763 1132 Size: 97 1302 209 192 286 1545 2229 Fragments arranged by size: 2229 1545 1302 286 209 192 97 PasI CC'CwG_GG Cuts at: 1348 2958 1348 Size: 1610 4250 PciI A'CATG_T Cuts at: 2736 4759 2736 Size: 2023 3837 PflMI CCAn_nnn'nTGG Cuts at: 1108 2374 2947 1108 Size: 1266 573 4021 Fragments arranged by size: 4021 1266 573 PfoI T'CCnGG_A Cuts at: 2633 3055 2633 Size: 422 5438 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2179 2676 2179 Size: 497 5363 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PspOMI G'GGCC_C Cuts at: 2108 2108 Size: 5860 PstI C_TGCA'G Cuts at: 830 2538 2980 3212 830 Size: 1708 442 232 3478 Fragments arranged by size: 3478 1708 442 232 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2936 2936 Size: 5860 SacI G_AGCT'C Cuts at: 2439 2439 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SanDI GG'GwC_CC Cuts at: 2676 2676 Size: 5860 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2534 2976 3208 4343 5024 5215 826 Size: 219 1489 442 232 1135 681 191 1471 Fragments arranged by size: 1489 1471 1135 681 442 232 219 191 SfoI GGC'GCC Cuts at: 2017 2017 Size: 5860 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 2037 2177 3187 1872 Size: 165 140 1010 4545 Fragments arranged by size: 4545 1010 165 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 3124 3964 4865 5127 820 Size: 197 926 1181 840 901 262 1553 Fragments arranged by size: 1553 1181 926 901 840 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 2531 3218 1132 Size: 1399 687 3774 Fragments arranged by size: 3774 1399 687 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 2004 2858 2970 3299 513 Size: 552 939 854 112 329 3074 Fragments arranged by size: 3074 939 854 552 329 112 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2950 3887 3904 4057 4242 4687 325 Size: 1255 1370 937 17 153 185 445 1498 Fragments arranged by size: 1498 1370 1255 937 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2685 4106 5818 Size: 274 80 33 51 1443 708 1421 1712 Cuts at: 5818 96 Size: 138 Fragments arranged by size: 1712 1443 1421 708 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2629 2661 5820 5852 2629 Size: 32 3159 32 2637 Fragments arranged by size: 3159 2637 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2026 2052 2026 Size: 26 5834 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 2035 2175 3185 1870 Size: 165 140 1010 4545 Fragments arranged by size: 4545 1010 165 140 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI