Restriction map for
pFN21AE0947
(Linear) (Six-base) MAPSORT of: KIEE0947.seq Check: 4409 from: 1 to: 1155
KIEE0947.orf
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AfeI AGC'GCT
Cuts at: 0 44 1155
Size: 44 1111
AflII C'TTAA_G
Cuts at: 0 1119 1155
Size: 1119 36
AflIII A'CryG_T
Cuts at: 0 731 1155
Size: 731 424
AlwNI CAG_nnn'CTG
Cuts at: 0 942 1155
Size: 942 213
ApaI G_GGCC'C
Cuts at: 0 107 1155
Size: 107 1048
ApoI r'AATT_y
Cuts at: 0 596 1155
Size: 596 559
AvaI C'yCGr_G
Cuts at: 0 30 106 170 1155
Size: 30 76 64 985
Fragments arranged by size:
985 76 64 30
BamHI G'GATC_C
Cuts at: 0 457 485 1155
Size: 457 28 670
Fragments arranged by size:
670 457 28
BanI G'GyrC_C
Cuts at: 0 10 1155
Size: 10 1145
BanII G_rGCy'C
Cuts at: 0 107 434 741 1155
Size: 107 327 307 414
Fragments arranged by size:
414 327 307 107
BbeI G_GCGC'C
Cuts at: 0 14 1155
Size: 14 1141
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 664 971 1058 1155
Size: 253 411 307 87 97
Fragments arranged by size:
411 307 253 97 87
BciVI GTATCCnnnnn_n'
Cuts at: 0 128 1155
Size: 128 1027
BclI T'GATC_A
Cuts at: 0 749 959 1155
Size: 749 210 196
Fragments arranged by size:
749 210 196
BglI GCCn_nnn'nGGC
Cuts at: 0 124 1155
Size: 124 1031
BglII A'GATC_T
Cuts at: 0 374 1155
Size: 374 781
BlpI GC'TnA_GC
Cuts at: 0 325 1155
Size: 325 830
Bme1580I G_kGCm'C
Cuts at: 0 107 1155
Size: 107 1048
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 448 1049 1155
Size: 448 601 106
Fragments arranged by size:
601 448 106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 979 1155
Size: 475 504 176
Fragments arranged by size:
504 475 176
Bpu10I CC'TnA_GC
Cuts at: 0 452 703 1155
Size: 452 251 452
Fragments arranged by size:
452 452 251
BsaHI Gr'CG_yC
Cuts at: 0 11 83 1155
Size: 11 72 1072
Fragments arranged by size:
1072 72 11
BsaWI w'CCGG_w
Cuts at: 0 421 1155
Size: 421 734
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1043 1155
Size: 743 300 112
Fragments arranged by size:
743 300 112
BsiHKAI G_wGCw'C
Cuts at: 0 434 1155
Size: 434 721
Bsp1286I G_dGCh'C
Cuts at: 0 107 434 741 1155
Size: 107 327 307 414
Fragments arranged by size:
414 327 307 107
BsrBI CCG'CTC
Cuts at: 0 471 1155
Size: 471 684
BsrDI GCAATG_nn'
Cuts at: 0 446 1155
Size: 446 709
BsrFI r'CCGG_y
Cuts at: 0 79 1155
Size: 79 1076
BstAPI GCAn_nnn'nTGC
Cuts at: 0 528 1155
Size: 528 627
BstXI CCAn_nnnn'nTGG
Cuts at: 0 726 1155
Size: 726 429
BstYI r'GATC_y
Cuts at: 0 374 457 485 1054 1155
Size: 374 83 28 569 101
Fragments arranged by size:
569 374 101 83 28
Bsu36I CC'TnA_GG
Cuts at: 0 692 1155
Size: 692 463
BtgI C'CryG_G
Cuts at: 0 965 1155
Size: 965 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 904 1155
Size: 904 251
BtsI GCAGTG_nn'
Cuts at: 0 731 1155
Size: 731 424
DraIII CAC_nnn'GTG
Cuts at: 0 667 1155
Size: 667 488
DrdI GACnn_nn'nnGTC
Cuts at: 0 172 1155
Size: 172 983
EaeI y'GGCC_r
Cuts at: 0 856 933 1155
Size: 856 77 222
Fragments arranged by size:
856 222 77
EarI CTCTTCn'nnn_
Cuts at: 0 581 1155
Size: 581 574
EcoICRI GAG'CTC
Cuts at: 0 432 1155
Size: 432 723
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 979 1155
Size: 475 504 176
Fragments arranged by size:
504 475 176
EcoO109I rG'GnC_Cy
Cuts at: 0 97 174 671 939 1028 1155
Size: 97 77 497 268 89 127
Fragments arranged by size:
497 268 127 97 89 77
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 728 1134 1155
Size: 728 406 21
Fragments arranged by size:
728 406 21
HaeII r_GCGC'y
Cuts at: 0 14 46 1155
Size: 14 32 1109
Fragments arranged by size:
1109 32 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 176 208 375 407 1155
Size: 176 32 167 32 748
Fragments arranged by size:
748 176 167 32 32
KasI G'GCGC_C
Cuts at: 0 10 1155
Size: 10 1145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1155
Size: 66 1089
MscI TGG'CCA
Cuts at: 0 858 935 1155
Size: 858 77 220
Fragments arranged by size:
858 220 77
MslI CAynn'nnrTG
Cuts at: 0 406 581 1155
Size: 406 175 574
Fragments arranged by size:
574 406 175
MspA1I CmG'CkG
Cuts at: 0 931 1155
Size: 931 224
NaeI GCC'GGC
Cuts at: 0 81 1155
Size: 81 1074
NarI GG'CG_CC
Cuts at: 0 11 1155
Size: 11 1144
NcoI C'CATG_G
Cuts at: 0 965 1155
Size: 965 190
NdeI CA'TA_TG
Cuts at: 0 756 1100 1155
Size: 756 344 55
Fragments arranged by size:
756 344 55
NgoMIV G'CCGG_C
Cuts at: 0 79 1155
Size: 79 1076
NspI r_CATG'y
Cuts at: 0 526 735 927 1155
Size: 526 209 192 228
Fragments arranged by size:
526 228 209 192
PasI CC'CwG_GG
Cuts at: 0 953 1155
Size: 953 202
PciI A'CATG_T
Cuts at: 0 731 1155
Size: 731 424
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 942 1155
Size: 369 573 213
Fragments arranged by size:
573 369 213
PfoI T'CCnGG_A
Cuts at: 0 628 1050 1155
Size: 628 422 105
Fragments arranged by size:
628 422 105
PpuMI rG'GwC_Cy
Cuts at: 0 174 671 1155
Size: 174 497 484
Fragments arranged by size:
497 484 174
PspOMI G'GGCC_C
Cuts at: 0 103 1155
Size: 103 1052
PstI C_TGCA'G
Cuts at: 0 533 975 1155
Size: 533 442 180
Fragments arranged by size:
533 442 180
PvuII CAG'CTG
Cuts at: 0 931 1155
Size: 931 224
SacI G_AGCT'C
Cuts at: 0 434 1155
Size: 434 721
SanDI GG'GwC_CC
Cuts at: 0 671 1155
Size: 671 484
SfcI C'TryA_G
Cuts at: 0 529 971 1155
Size: 529 442 184
Fragments arranged by size:
529 442 184
SfoI GGC'GCC
Cuts at: 0 12 1155
Size: 12 1143
SmaI CCC'GGG
Cuts at: 0 32 172 1155
Size: 32 140 983
Fragments arranged by size:
983 140 32
SmlI C'TyrA_G
Cuts at: 0 1119 1155
Size: 1119 36
SphI G_CATG'C
Cuts at: 0 526 1155
Size: 526 629
StyI C'CwwG_G
Cuts at: 0 853 965 1155
Size: 853 112 190
Fragments arranged by size:
853 190 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 945 1155
Size: 945 210
TatI w'GTAC_w
Cuts at: 0 680 1155
Size: 680 475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 624 656 1155
Size: 624 32 499
Fragments arranged by size:
624 499 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 21 47 1155
Size: 21 26 1108
Fragments arranged by size:
1108 26 21
XmaI C'CCGG_G
Cuts at: 0 30 170 1155
Size: 30 140 985
Fragments arranged by size:
985 140 30
Enzymes that do cut:
AfeI AflII AflIII AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BplI BpmI Bpu10I BsaHI BsaWI BsgI
BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PpuMI PspOMI PstI
PvuII SacI SanDI SfcI SfoI SmaI SmlI SphI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmrI BmtI BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI CspCI DraI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmnI ZraI