################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AflII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BbvI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI Fnu4HI FseI FspI HaeII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII ItaI KasI Ksp632I KspI MaeI MaeII MamI MboII MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TseI TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 19 GGTACC G^GTACC AcsI 208 GAATTT [GA]^AATT[CT] AcyI 364 GGCGTC G[GA]^CG[CT]C AflIII 279 ACATGT A^C[GA][CT]GT ApoI 208 GAATTT [GA]^AATT[CT] Asp718I 19 GGTACC G^GTACC AspHI 246 GAGCAC G[AT]GC[AT]^C AspI 214 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 107 TGGCCA TGG^CCA BanI 19 GGTACC G^G[CT][GA]CC BcgI 216 CGAAGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BmyI 246 GAGCAC G[AGT]GC[ACT]^C BpmI 36 CTCCAG CTGGAG(16/14) BsaHI 364 GGCGTC G[GA]^CG[CT]C BsgI 283 GTGCAG GTGCAG(16/14) BsiHKAI 246 GAGCAC G[AT]GC[AT]^C BsiYI 257 CCTTAGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 257 CCTTAGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 34 GTCTC GTCTC(1/5) BsmI 349 GAATGC GAATGC(1/-1) Bsp1286I 246 GAGCAC G[AGT]GC[ACT]^C BspHI 356 TCATGA T^CATGA BspLU11I 279 ACATGT A^CATGT BssSI 375 CACGAG CACGAG(-5/-1) BstXI 89 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 100 GCTGGC GC[ACGT]^[ACGT]GC Cfr13I 201 GGGCC G^G[ACGT]CC DraI 172 TTTAAA TTT^AAA DrdI 232 GACAGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 107 TGGCCA [CT]^GGCC[GA] Hin1I 364 GGCGTC G[GA]^CG[CT]C HphI 149 TCACC GGTGA(8/7) KpnI 19 GGTACC GGTAC^C MluNI 107 TGGCCA TGG^CCA MnlI 328 GAGG CCTC(7/6) MscI 107 TGGCCA TGG^CCA MslI 126 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 100 GCTGGCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 19 GGTACC GG[ACGT]^[ACGT]CC NspI 279 ACATGT [GA]CATG^[CT] PflFI 214 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 224 CTGCAG CTGCA^G RcaI 356 TCATGA T^CATGA Sau96I 201 GGGCC G^G[ACGT]CC SfaNI 7 GATGC GCATC(5/9) SfcI 224 CTGCAG C^T[GA][CT]AG Tsp509I 209 AATT ^AATT Tth111I 214 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 86 CCACCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BclI 122 TGATCA T^GATCA 146 TGATCA DdeI 182 CTGAG C^T[ACGT]AG 258 CTTAG DpnII 123 GATC ^GATC 147 GATC FbaI 122 TGATCA T^GATCA 146 TGATCA HaeIII 108 GGCC GG^CC 202 GGCC HgaI 353 GCGTC GACGC(5/10) 365 GCGTC MaeIII 220 GTCAC ^GT[ACGT]AC 367 GTCAC MboI 123 GATC ^GATC 147 GATC MseI 155 TTAA T^TAA 173 TTAA NdeII 123 GATC ^GATC 147 GATC Sau3AI 123 GATC ^GATC 147 GATC Tru9I 155 TTAA T^TAA 173 TTAA Tsp45I 220 GTCAC ^GT[GC]AC 367 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AfaI BsrI BssKI BstF5I BstNI EcoRII FokI MvaI NlaIII RsaI ScrFI TspRI