################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I EcoT22I FokI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI Tsp45I TspRI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AspI 660 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 103 GGACC G^G[AT]CC BclI 291 TGATCA T^GATCA BfaI 737 CTAG C^TAG BsaAI 437 CACGTA [CT]AC^GT[GA] BsmAI 666 GTCTC GTCTC(1/5) BsmI 956 GCATTC GAATGC(1/-1) BsrDI 261 CATTGC GCAATG(2/0) BstBI 959 TTCGAA TT^CGAA BstYI 556 GGATCT [GA]^GATC[CT] Cac8I 586 GCTGGC GC[ACGT]^[ACGT]GC CfoI 584 GCGC GCG^C DdeI 794 CTGAG C^T[ACGT]AG DraII 296 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 13 GAAGAG CTCTTC(1/4) EcoO109I 296 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 256 GAATTC G^AATTC FauI 514 CCCGC CCCGC(4/6) FbaI 291 TGATCA T^GATCA HaeII 583 GGCGCT [GA]GCGC^[CT] HhaI 584 GCGC GCG^C HinP1I 584 GCGC G^CGC HphI 874 TCACC GGTGA(8/7) Ksp632I 13 GAAGAG CTCTTC(1/4) MaeI 737 CTAG C^TAG MaeII 438 ACGT A^CGT MaeIII 48 GTTAC ^GT[ACGT]AC MflI 556 GGATCT [GA]^GATC[CT] MslI 901 CATGAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 398 CAGCTG C[AC]G^C[GT]G MwoI 691 GCCCCTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 103 GGACCC GG[ACGT]^[ACGT]CC NspV 959 TTCGAA TT^CGAA PflFI 660 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 398 CAGCTG CAG^CTG SfuI 959 TTCGAA TT^CGAA SspI 850 AATATT AAT^ATT StuI 285 AGGCCT AGG^CCT TaqI 960 TCGA T^CGA Tth111I 660 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 960 TCGA T^CGA XhoII 556 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AciI 515 CCGC CCGC(-3/-1) 571 CCGC AlwI 346 GATCC GGATC(4/5) 556 GGATC BsaJI 205 CCGAGG C^C[ACGT][ACGT]GG 759 CCCAGG BsrI 9 ACTGG ACTGG(1/-1) 82 CCAGT BstXI 229 CCATCTAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 900 CCATGAGTGTGG SfaNI 547 GCATC GCATC(5/9) 829 GCATC SfcI 464 CTACAG C^T[GA][CT]AG 923 CTGTAG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AcsI AluI ApoI BbsI BbvI BpuAI BseRI BsiYI BslI BsmFI BssKI BstNI Cfr13I DpnII EcoNI EcoRII Fnu4HI HaeIII HinfI ItaI MboI MboII MnlI MseI MvaI NdeII NlaIII Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp509I