(Linear) (Six-base) MAPSORT of: KIEE2784.seq Check: 4480 from: 1 to: 975 KIEE2784.orf With 182 enzymes: SgfI * February 19, 2021 20:23 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 596 640 975 Size: 188 408 44 335 Fragments arranged by size: 408 335 188 44 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 63 95 165 197 975 Size: 63 32 70 32 778 Fragments arranged by size: 778 70 63 32 32 ApoI r'AATT_y Cuts at: 0 256 414 457 531 723 892 937 975 Size: 256 158 43 74 192 169 45 38 Fragments arranged by size: 256 192 169 158 74 45 43 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 124 145 617 975 Size: 124 21 472 358 Fragments arranged by size: 472 358 124 21 BclI T'GATC_A Cuts at: 0 291 975 Size: 291 684 BsaAI yAC'GTr Cuts at: 0 439 975 Size: 439 536 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 14 44 651 681 695 725 975 Size: 14 30 607 30 14 30 250 Fragments arranged by size: 607 250 30 30 30 14 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 314 317 320 393 975 Size: 314 3 3 73 582 Fragments arranged by size: 582 314 73 3 3 BsmI GAATG_Cn' Cuts at: 0 956 975 Size: 956 19 BsrDI GCAATG_nn' Cuts at: 0 260 975 Size: 260 715 BstBI TT'CG_AA Cuts at: 0 960 975 Size: 960 15 BstXI CCAn_nnnn'nTGG Cuts at: 0 236 907 975 Size: 236 671 68 Fragments arranged by size: 671 236 68 BstYI r'GATC_y Cuts at: 0 556 975 Size: 556 419 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 45 80 291 326 722 757 975 Size: 45 35 211 35 396 35 218 Fragments arranged by size: 396 218 211 45 35 35 35 EarI CTCTTCn'nnn_ Cuts at: 0 8 975 Size: 8 967 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 596 640 975 Size: 188 408 44 335 Fragments arranged by size: 408 335 188 44 EcoNI CCTnn'n_nnAGG Cuts at: 0 292 304 698 758 975 Size: 292 12 394 60 217 Fragments arranged by size: 394 292 217 60 12 EcoO109I rG'GnC_Cy Cuts at: 0 297 975 Size: 297 678 EcoRI G'AATT_C Cuts at: 0 256 975 Size: 256 719 HaeII r_GCGC'y Cuts at: 0 587 975 Size: 587 388 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 14 46 651 683 695 727 814 846 Size: 14 32 605 32 12 32 87 32 Cuts at: 846 975 Size: 129 Fragments arranged by size: 605 129 87 32 32 32 32 14 12 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 18 208 975 Size: 18 190 767 Fragments arranged by size: 767 190 18 MslI CAynn'nnrTG Cuts at: 0 905 975 Size: 905 70 MspA1I CmG'CkG Cuts at: 0 400 975 Size: 400 575 PfoI T'CCnGG_A Cuts at: 0 537 975 Size: 537 438 PvuII CAG'CTG Cuts at: 0 400 975 Size: 400 575 SfcI C'TryA_G Cuts at: 0 464 923 975 Size: 464 459 52 Fragments arranged by size: 464 459 52 SspI AAT'ATT Cuts at: 0 852 975 Size: 852 123 StuI AGG'CCT Cuts at: 0 287 975 Size: 287 688 Tth111I GACn'n_nGTC Cuts at: 0 663 975 Size: 663 312 Enzymes that do cut: AcuI AloI ApoI BbsI BclI BsaAI BsaXI BseRI BsmI BsrDI BstBI BstXI BstYI CspCI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MmeI MslI MspA1I PfoI PvuII SfcI SspI StuI Tth111I Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StyI SwaI TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI