################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbsI BbvCI BcgI BciVI BfrI BglI BlnI BpmI BpuAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MluI MroI MslI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AclI 496 AACGTT AA^CGTT AvaI 953 CCCGGG C^[CT]CG[GA]G BalI 755 TGGCCA TGG^CCA BanII 629 GAGCTC G[GA]GC[CT]^C BbrPI 1005 CACGTG CAC^GTG BclI 609 TGATCA T^GATCA BfaI 769 CTAG C^TAG BlpI 867 GCTAAGC GC^T[ACGT]AGC Bpu1102I 867 GCTAAGC GC^T[ACGT]AGC BsaAI 1005 CACGTG [CT]AC^GT[GA] BsaI 449 GGTCTC GGTCTC(1/5) BsgI 907 GTGCAG GTGCAG(16/14) BsmI 227 GCATTC GAATGC(1/-1) BsoBI 953 CCCGGG C^[CT]CG[GA]G Bsp1407I 354 TGTACA T^GTACA BspMI 254 GCAGGT ACCTGC(4/8) BsrBI 332 GAGCGG CCGCTC(-3/-3) BsrDI 267 GCAATG GCAATG(2/0) BsrGI 354 TGTACA T^GTACA BstAPI 645 GCACTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 475 CCTGG CC^[AT]GG CelII 867 GCTAAGC GC^T[ACGT]AGC DraI 828 TTTAAA TTT^AAA DraII 1179 AGGTCCC [GA]G^G[ACGT]CC[CT] DrdI 124 GACAACGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 755 TGGCCA [CT]^GGCC[GA] EcoO109I 1179 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoRII 475 CCTGG ^CC[AT]GG EcoT22I 1129 ATGCAT ATGCA^T FauI 334 GCGGG CCCGC(4/6) FbaI 609 TGATCA T^GATCA HaeIII 756 GGCC GG^CC HapII 954 CCGG C^CGG HgaI 221 GACGC GACGC(5/10) HpaII 954 CCGG C^CGG MaeI 769 CTAG C^TAG MluNI 755 TGGCCA TGG^CCA MscI 755 TGGCCA TGG^CCA MspI 954 CCGG C^CGG MvaI 475 CCTGG CC^[AT]GG NsiI 1129 ATGCAT ATGCA^T PflMI 1153 CCATTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1005 CACGTG CAC^GTG PmlI 1005 CACGTG CAC^GTG PpuMI 1179 AGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 496 AACGTT AA^CGTT PstI 994 CTGCAG CTGCA^G SacI 629 GAGCTC GAGCT^C SfcI 994 CTGCAG C^T[GA][CT]AG SmaI 953 CCCGGG CCC^GGG SspBI 354 TGTACA T^GTACA TaqI 312 TCGA T^CGA TthHB8I 312 TCGA T^CGA Van91I 1153 CCATTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 768 TCTAGA T^CTAGA XmaCI 953 CCCGGG C^CCGGG XmaI 953 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 723 CCGC CCGC(-3/-1) 334 GCGG AfaI 355 GTAC GT^AC 1269 GTAC AspHI 629 GAGCTC G[AT]GC[AT]^C 643 GAGCAC AvaII 30 GGACC G^G[AT]CC 1180 GGTCC BcnI 953 CCCGG CC^[GC]GG 954 CCGGG BglII 458 AGATCT A^GATCT 1211 AGATCT BmyI 629 GAGCTC G[AGT]GC[ACT]^C 643 GAGCAC BsiHKAI 629 GAGCTC G[AT]GC[AT]^C 643 GAGCAC BsmFI 158 GGGAC GGGAC(10/14) 1181 GTCCC Bsp1286I 629 GAGCTC G[AGT]GC[ACT]^C 643 GAGCAC Cfr13I 30 GGACC G^G[ACGT]CC 1180 GGTCC HphI 520 GGTGA GGTGA(8/7) 720 TCACC MaeIII 239 GTCAC ^GT[ACGT]AC 368 GTGAC MboII 386 GAAGA GAAGA(8/7) 215 TCTTC MfeI 836 CAATTG C^AATTG 1330 CAATTG MspA1I 192 CAGCTG C[AC]G^C[GT]G 729 CAGCTG MunI 836 CAATTG C^AATTG 1330 CAATTG NciI 953 CCCGG CC^[GC]GG 954 CCGGG PleI 949 GACTC GAGTC(4/5) 237 GAGTC PvuII 192 CAGCTG CAG^CTG 729 CAGCTG RsaI 355 GTAC GT^AC 1269 GTAC Sau96I 30 GGACC G^G[ACGT]CC 1180 GGTCC SmlI 468 CTCAAG C^T[CT][GA]AG 1145 CTCAAG Tsp45I 239 GTCAC ^GT[GC]AC 368 GTGAC XcmI 419 CCATTAACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1184 CCATGTCAACACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AluI AlwI BbvI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstYI DdeI DpnII Fnu4HI FokI HincII HindII HinfI ItaI MaeII MboI MflI MnlI MseI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI XhoII