(Circular) (Six-base) MAPSORT of: pFN21AB7751.seq Check: 1035 from: 1 to: 6040 pFN21AB7751 6040 bp With 182 enzymes: SgfI * March 28, 2012 13:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 3361 3361 Size: 6040 AclI AA'CG_TT Cuts at: 1563 2502 4167 4540 1563 Size: 939 1665 373 3063 Fragments arranged by size: 3063 1665 939 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2206 2466 3062 4103 5487 5755 1632 Size: 574 260 596 1041 1384 268 1917 Fragments arranged by size: 1917 1384 1041 596 574 268 260 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 4939 5916 1203 Size: 3736 977 1327 Fragments arranged by size: 3736 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 3783 5355 3783 Size: 1572 4468 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 3349 3575 3727 5836 1493 Size: 1856 226 152 2109 1697 Fragments arranged by size: 2109 1856 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4594 160 Size: 872 3562 1606 Fragments arranged by size: 3562 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2958 3365 1870 Size: 73 1015 407 4545 Fragments arranged by size: 4545 1015 407 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3361 4816 618 Size: 325 217 627 1574 1455 1842 Fragments arranged by size: 1842 1574 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2638 3359 1391 Size: 1247 721 4072 Fragments arranged by size: 4072 1247 721 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5069 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3932 5142 3932 Size: 1210 4830 BclI T'GATC_A Cuts at: 1548 2614 1548 Size: 1066 4974 BfrBI ATG'CAT Cuts at: 3136 3136 Size: 6040 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4651 136 Size: 107 122 71 631 605 2979 1525 Fragments arranged by size: 2979 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2463 3216 6035 2463 Size: 753 2819 2468 Fragments arranged by size: 2819 2468 753 BlpI GC'TnA_GC Cuts at: 2873 3457 2873 Size: 584 5456 Bme1580I G_kGCm'C Cuts at: 1790 4101 5257 1790 Size: 2311 1156 2573 Fragments arranged by size: 2573 2311 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3771 4729 442 Size: 416 53 83 399 24 2354 958 1753 Fragments arranged by size: 2354 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4700 1239 Size: 3461 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2458 3135 4165 5030 5328 2458 Size: 677 1030 865 298 3170 Fragments arranged by size: 3170 1030 865 677 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2460 4703 882 Size: 617 961 2243 2219 Fragments arranged by size: 2243 2219 961 617 BsaAI yAC'GTr Cuts at: 493 1204 3012 3831 493 Size: 711 1808 819 2702 Fragments arranged by size: 2702 1808 819 711 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3847 4229 4864 275 Size: 53 83 186 936 2314 382 635 1451 Fragments arranged by size: 2314 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4473 4871 5145 5292 5703 4473 Size: 398 274 147 411 4810 Fragments arranged by size: 4810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 1178 Size: 30 165 30 1002 30 4783 Fragments arranged by size: 4783 1002 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6040 BseYI C'CCAG_C Cuts at: 5243 5739 5243 Size: 496 5544 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2933 1163 Size: 573 1197 4270 Fragments arranged by size: 4270 1197 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2638 2652 4101 4186 5257 2638 Size: 14 1449 85 1071 3421 Fragments arranged by size: 3421 1449 1071 85 14 BsmI GAATG_Cn' Cuts at: 2232 3551 3644 2232 Size: 1319 93 4628 Fragments arranged by size: 4628 1319 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2638 2652 3359 4101 4186 5257 1391 Size: 399 848 14 707 742 85 1071 2174 Fragments arranged by size: 2174 1071 848 742 707 399 85 14 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2250 3395 844 Size: 1406 1145 3489 Fragments arranged by size: 3489 1406 1145 BsrBI CCG'CTC Cuts at: 1394 1823 2339 3927 1394 Size: 429 516 1588 3507 Fragments arranged by size: 3507 1588 516 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2279 4535 4709 65 Size: 1183 1031 2256 174 1396 Fragments arranged by size: 2256 1396 1183 1031 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4684 4871 1700 Size: 110 2874 187 2869 Fragments arranged by size: 2874 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2359 96 Size: 2263 3777 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 2656 794 Size: 1862 4178 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6040 BstYI r'GATC_y Cuts at: 1972 2463 2675 3216 3370 3737 4122 4139 5591 Size: 491 212 541 154 367 385 17 1452 Cuts at: 5591 5677 5689 6035 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 541 491 385 367 346 212 154 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4549 Fragments arranged by size: 4549 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 2996 3556 4350 4370 2996 Size: 560 794 20 4666 Fragments arranged by size: 4666 794 560 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6005 DraI TTT'AAA Cuts at: 2835 3344 2835 Size: 509 5531 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2135 5047 809 Size: 507 819 2912 1802 Fragments arranged by size: 2912 1802 819 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2760 3487 4376 Size: 54 1425 50 275 18 930 727 889 Cuts at: 4376 6005 8 Size: 1629 43 Fragments arranged by size: 1629 1425 930 889 727 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3970 1353 Size: 2617 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2636 2636 Size: 6040 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2206 2466 3062 4103 4700 5487 5755 Size: 393 574 260 596 1041 597 787 268 Cuts at: 5755 1239 Size: 1524 Fragments arranged by size: 1524 1041 787 597 596 574 393 268 260 EcoO109I rG'GnC_Cy Cuts at: 3185 3185 Size: 6040 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 6040 FspI TGC'GCA Cuts at: 4546 4546 Size: 6040 HaeII r_GCGC'y Cuts at: 1678 5187 1678 Size: 3509 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3203 3208 3235 3240 4681 Size: 32 135 32 1631 5 27 5 1441 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1631 1441 135 42 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2140 2284 3195 3384 3630 669 Size: 988 281 202 144 911 189 246 3079 Fragments arranged by size: 3079 988 911 281 246 202 189 144 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 3365 3365 Size: 6040 MfeI C'AATT_G Cuts at: 2841 3335 3639 2841 Size: 494 304 5242 Fragments arranged by size: 5242 494 304 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2762 10 Size: 54 1425 343 930 3288 Fragments arranged by size: 3288 1425 930 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3998 4357 4516 518 Size: 648 843 1989 359 159 2042 Fragments arranged by size: 2042 1989 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2199 2736 3456 4133 5281 5526 1606 Size: 593 537 720 677 1148 245 2120 Fragments arranged by size: 2120 1148 720 677 593 537 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4549 Fragments arranged by size: 4549 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6040 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NsiI A_TGCA'T Cuts at: 3138 3138 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 3398 4943 1132 Size: 97 2169 1545 2229 Fragments arranged by size: 2229 2169 1545 97 PasI CC'CwG_GG Cuts at: 1348 2480 1348 Size: 1132 4908 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 3164 1108 Size: 2056 3984 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PmlI CAC'GTG Cuts at: 3012 3012 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3185 3185 Size: 6040 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PstI C_TGCA'G Cuts at: 830 3003 3392 830 Size: 2173 389 3478 Fragments arranged by size: 3478 2173 389 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 2199 2736 2199 Size: 537 5503 SacI G_AGCT'C Cuts at: 2638 2638 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 2999 3388 4523 5204 5395 826 Size: 219 1954 389 1135 681 191 1471 Fragments arranged by size: 1954 1471 1135 681 389 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 2960 3367 1872 Size: 1088 407 4545 Fragments arranged by size: 4545 1088 407 SmlI C'TyrA_G Cuts at: 820 1017 1943 2473 3150 4144 5045 5307 820 Size: 197 926 530 677 994 901 262 1553 Fragments arranged by size: 1553 994 926 901 677 530 262 197 SnaBI TAC'GTA Cuts at: 493 3831 493 Size: 3338 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 2004 3479 513 Size: 552 939 1475 3074 Fragments arranged by size: 3074 1475 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2196 2442 4067 4084 4237 4422 4867 Size: 1255 616 246 1625 17 153 185 445 Cuts at: 4867 325 Size: 1498 Fragments arranged by size: 1625 1498 1255 616 445 246 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2359 3273 4286 Size: 274 80 33 51 1443 382 914 1013 Cuts at: 4286 5998 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1013 914 382 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 2773 3376 2773 Size: 603 5437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2431 3196 2431 Size: 765 5275 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 2958 3365 1870 Size: 1088 407 4545 Fragments arranged by size: 4545 1088 407 XmnI GAAnn'nnTTC Cuts at: 4169 4169 Size: 6040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI