################################## # NO CUT ENZYMES ( 212 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI Hin1I HindIII HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 23 enzymes) # ################################### AcsI 373 AAATTT [GA]^AATT[CT] ApoI 373 AAATTT [GA]^AATT[CT] AseI 136 ATTAAT AT^TAAT AsnI 136 ATTAAT AT^TAAT AspHI 317 GAGCTC G[AT]GC[AT]^C BamHI 166 GGATCC G^GATCC BsiHKAI 317 GAGCTC G[AT]GC[AT]^C BsmAI 331 GAGAC GTCTC(1/5) CfoI 267 GCGC GCG^C DraI 210 TTTAAA TTT^AAA EcoT14I 199 CCAAGG C^C[AT][AT]GG HhaI 267 GCGC GCG^C HinP1I 267 GCGC G^CGC HinfI 255 GAATC G^A[ACGT]TC HpaI 142 GTTAAC GTT^AAC MaeIII 3 GTTAC ^GT[ACGT]AC MnlI 287 GAGG CCTC(7/6) NlaIII 252 CATG CATG^ PshBI 136 ATTAAT AT^TAAT SacI 317 GAGCTC GAGCT^C SfaNI 85 GATGC GCATC(5/9) StyI 199 CCAAGG C^C[AT][AT]GG TfiI 255 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BanII 317 GAGCTC G[GA]GC[CT]^C 420 GAGCCC BmyI 317 GAGCTC G[AGT]GC[ACT]^C 420 GAGCCC BsaJI 199 CCAAGG C^C[ACGT][ACGT]GG 285 CCGAGG Bsp1286I 317 GAGCTC G[AGT]GC[ACT]^C 420 GAGCCC BstF5I 80 CATCC GGATG(2/0) 456 CATCC BstYI 166 GGATCC [GA]^GATC[CT] 289 GGATCT DdeI 53 CTCAG C^T[ACGT]AG 380 CTAAG DpnII 167 GATC ^GATC 290 GATC FokI 80 CATCC GGATG(9/13) 456 CATCC HincII 142 GTTAAC GT[CT]^[GA]AC 244 GTCAAC HindII 142 GTTAAC GT[CT]^[GA]AC 244 GTCAAC MboI 167 GATC ^GATC 290 GATC MflI 166 GGATCC [GA]^GATC[CT] 289 GGATCT NdeII 167 GATC ^GATC 290 GATC NlaIV 166 GGATCC GG[ACGT]^[ACGT]CC 419 GGAGCC Sau3AI 167 GATC ^GATC 290 GATC XhoII 166 GGATCC [GA]^GATC[CT] 289 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### AluI AlwI MboII MseI Tru9I Tsp509I